LOCUS Exported 4436 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4436 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 2473..2520 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 4368..4384 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 4385..4416 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 29..2340 /label="CD_OpenVent" /note="" primer_bind complement(2525..2541) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(2608..3196) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3387..4247) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(4248..4352) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 2376..2435 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AACGATGATG CTCACTCTCG GGTCTCAAAT GATCATTGAC GCTGACTACA TCACTGAAGA 61 TGGCAAACCG ATCATCCGTA TTTTTAAAAA AGAGAAAGGT GAATTCAAAG TCGAATACGA 121 CCGCACCTTT CGCCCGTACA TTTACGCGCT GCTGAAAGAT GACAGCGCTA TTGATGAAGT 181 GAAGAAAATT ACTGCGGAAC GTCATGGTAA AATTGTTCGT ATTACGGAGG TGGAAAAAGT 241 CCAAAAAAAA TTCCTGGGCC GTCCTATCGA GGTGTGGAAG CTGTACCTGG AACATCCTCA 301 GGATGTTCCG GCAATTCGCG AAAAAATCCG TGAACACCCG GCCGTCGTAG ATATCTTCGA 361 GTACGATATC CCGTTCGCAA AACGTTACCT GATTGATAAA GGCCTGACGC CGATGGAGGG 421 CAACGAAGAA CTGACCTTTC TGGCGGTGGA CATCGAAACT CTGTATCACG AAGGCGAAGA 481 ATTTGGTAAA GGCCCTATTA TCATGATTTC GTATGCGGAT GAGGAAGGAG CGAAAGTGAT 541 CACATGGAAA AGCATTGACC TGCCGTACGT TGAAGTGGTC AGCTCAGAAC GCGAAATGAT 601 TAAACGCCTC GTTAAAGTCA TTCGTGAAAA GGATCCGGAC GTAATCATCA CCTACAATGG 661 TGATAACTTC GACTTTCCGT ATCTTCTGAA ACGTGCGGAA AAATTGGGCA TTAAACTGCC 721 GCTTGGTCGT GACAACAGCG AACCGAAAAT GCAGCGCATG GGTGACAGCC TGGCGGTGGA 781 AATTAAAGGT CGTATCCACT TCGACCTGTT CCCGGCGATC CGTCGTACCA TCAACCTGCC 841 GACGTATACC CTGGAAACGG TTTACGAAGT TATTTTCGGC AAATCCAAAG AAAAAGTTTA 901 CGCTCACGAA ATCGCGGAAG CATGGGAAAC CGGTAAAGGC CTGGAACGTG TGGCAAAGTA 961 CAGCATGGAA GATGCAAAAG TGACCAGCGA ACTGGGGAAA GAATTCTTTC CAATGGAAGC 1021 GCAACTGGCA CGCCTGGTGG GTCATCCGGT CTGGGACGTG TCTCGTAGCT CGACTGGCAA 1081 CCTGGTGGAA TGGTTCCTGC TGACTAAAGC TTACGAACGC AACGAGCTCG CACCGAACAA 1141 ACCGGATGAA CGTGAATACG AACGCCGTCT GCGTGAGAGC TACGAAGGCG GTTACGTGAA 1201 CGAACCGGAA AAAGGCCTGT GGGAGGGTAT CGTGAGCCTG GATTTCCGTA GCCTGTACCC 1261 GTCGATTATC ATCACTCATA ACGTATCCCC AGATACGCTC AACCGTGAAA ACTGTAAAGA 1321 ATATGACGTT GCGCCTCAGG TGGGTCATCG CTTCTGCAAG GATTTTCCAG GTTTCATCCC 1381 GAGCCTGCTG GGAAACCTGC TGGAGGAACG TCAAAAAATC AAAAAACGCA TGAAAGAGAG 1441 TAAAGACCCG GTGGAAAAAA AGCTGCTGGA CTACCGTCAG CGTGCTATTA AGATCCTTGC 1501 GAACTCTTAT TACGGTTATT ACGGCTATGC AAAAGCGCGT TGGTACTGCA AAGAATGCGC 1561 TGAATCCGTT ACTGCATGGG GTCGTCAGTA CATCGACCTG GTACGTAGGG AACTGGAGAG 1621 CCGTGGCTTC AAAGTACTGT ATATCGACAC TGATGGTCTG TACGCCACTA TCCCGGGCGC 1681 AAAACATGAA GAGATCAAAG AGAAAGCCCT GAAATTCGTC GAGTATATAA ACTCCAAACT 1741 CCCGGGCCTG CTGGAACTGG AATATGAAGG CTTCTACGCG CGCGGCTTCT TTGTGACCAA 1801 AAAAAAATAC GCTCTGATTG ATGAGGAAGG TAAAATAGTT ACCCGCGGCC TGGAAATCGT 1861 GCGCCGTGAC TGGAGCGAAA TCGCGAAAGA GACTCAGGCA AAAGTTCTGG AAGCAATCCT 1921 GAAGCACGGC AACGTTGACG AGGCGGTGAA AATCGTAAAG GAAGTTACCG AAAAGCTGTC 1981 GAAGTATGAG ATCCCACCAG AAAAACTGGT TATTTACGAG CAAATCACCC GCCCGTTATC 2041 AGAATACAAA GCAATTGGCC CGCATGTAGC TGTGGCCAAA CGCCTGGCCG CGAAAGGCGT 2101 AAAAGTTAAA CCGGGTATGG TAATTGGGTA CATCGTCCTG AAGGGAGATG GTCCAATCAG 2161 CAAACGTGCC ATTGCTATTG AAGAGTTTGA TCCGAAAAAA CACAAATACG ACGCCGAATA 2221 CTATATTGAA AACCAAGTTC TGCCGGCCGT TGAACGTATC CTGCGCGCGT TCGGCTACCG 2281 TAAGGAGGAT CTGCGCTACC AAAAAACCAA ACAGGTGGGT CTGGGTGCTT GGCTGAAATT 2341 CGGAGGTCGA GACCACCGGT TGTACCTATC GAGTGCCAGC TACAAAAACG TGGACGTAGG 2401 GATAACAGGG TAATGGGCGA CGAGAGGACT GTATACGCTG AGGTGTCAAT CGTCGGAGCC 2461 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG 2521 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT 2581 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 2641 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 2701 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 2761 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 2821 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 2881 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 2941 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 3001 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 3061 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 3121 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 3181 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG 3241 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 3301 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 3361 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 3421 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 3481 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA 3541 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 3601 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 3661 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA 3721 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA 3781 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA 3841 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT 3901 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA 3961 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG 4021 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC 4081 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA 4141 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT 4201 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT 4261 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 4321 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC 4381 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAG //