LOCUS       Exported     4436 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4436
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2473..2520
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4368..4384
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4385..4416
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..2340
                     /label="CD_OpenVent"
                     /note=""
     primer_bind     complement(2525..2541)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2608..3196)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3387..4247)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4248..4352)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    2376..2435
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GATCATTGAC GCTGACTACA TCACTGAAGA
       61 TGGCAAACCG ATCATCCGTA TTTTTAAAAA AGAGAAAGGT GAATTCAAAG TCGAATACGA
      121 CCGCACCTTT CGCCCGTACA TTTACGCGCT GCTGAAAGAT GACAGCGCTA TTGATGAAGT
      181 GAAGAAAATT ACTGCGGAAC GTCATGGTAA AATTGTTCGT ATTACGGAGG TGGAAAAAGT
      241 CCAAAAAAAA TTCCTGGGCC GTCCTATCGA GGTGTGGAAG CTGTACCTGG AACATCCTCA
      301 GGATGTTCCG GCAATTCGCG AAAAAATCCG TGAACACCCG GCCGTCGTAG ATATCTTCGA
      361 GTACGATATC CCGTTCGCAA AACGTTACCT GATTGATAAA GGCCTGACGC CGATGGAGGG
      421 CAACGAAGAA CTGACCTTTC TGGCGGTGGA CATCGAAACT CTGTATCACG AAGGCGAAGA
      481 ATTTGGTAAA GGCCCTATTA TCATGATTTC GTATGCGGAT GAGGAAGGAG CGAAAGTGAT
      541 CACATGGAAA AGCATTGACC TGCCGTACGT TGAAGTGGTC AGCTCAGAAC GCGAAATGAT
      601 TAAACGCCTC GTTAAAGTCA TTCGTGAAAA GGATCCGGAC GTAATCATCA CCTACAATGG
      661 TGATAACTTC GACTTTCCGT ATCTTCTGAA ACGTGCGGAA AAATTGGGCA TTAAACTGCC
      721 GCTTGGTCGT GACAACAGCG AACCGAAAAT GCAGCGCATG GGTGACAGCC TGGCGGTGGA
      781 AATTAAAGGT CGTATCCACT TCGACCTGTT CCCGGCGATC CGTCGTACCA TCAACCTGCC
      841 GACGTATACC CTGGAAACGG TTTACGAAGT TATTTTCGGC AAATCCAAAG AAAAAGTTTA
      901 CGCTCACGAA ATCGCGGAAG CATGGGAAAC CGGTAAAGGC CTGGAACGTG TGGCAAAGTA
      961 CAGCATGGAA GATGCAAAAG TGACCAGCGA ACTGGGGAAA GAATTCTTTC CAATGGAAGC
     1021 GCAACTGGCA CGCCTGGTGG GTCATCCGGT CTGGGACGTG TCTCGTAGCT CGACTGGCAA
     1081 CCTGGTGGAA TGGTTCCTGC TGACTAAAGC TTACGAACGC AACGAGCTCG CACCGAACAA
     1141 ACCGGATGAA CGTGAATACG AACGCCGTCT GCGTGAGAGC TACGAAGGCG GTTACGTGAA
     1201 CGAACCGGAA AAAGGCCTGT GGGAGGGTAT CGTGAGCCTG GATTTCCGTA GCCTGTACCC
     1261 GTCGATTATC ATCACTCATA ACGTATCCCC AGATACGCTC AACCGTGAAA ACTGTAAAGA
     1321 ATATGACGTT GCGCCTCAGG TGGGTCATCG CTTCTGCAAG GATTTTCCAG GTTTCATCCC
     1381 GAGCCTGCTG GGAAACCTGC TGGAGGAACG TCAAAAAATC AAAAAACGCA TGAAAGAGAG
     1441 TAAAGACCCG GTGGAAAAAA AGCTGCTGGA CTACCGTCAG CGTGCTATTA AGATCCTTGC
     1501 GAACTCTTAT TACGGTTATT ACGGCTATGC AAAAGCGCGT TGGTACTGCA AAGAATGCGC
     1561 TGAATCCGTT ACTGCATGGG GTCGTCAGTA CATCGACCTG GTACGTAGGG AACTGGAGAG
     1621 CCGTGGCTTC AAAGTACTGT ATATCGACAC TGATGGTCTG TACGCCACTA TCCCGGGCGC
     1681 AAAACATGAA GAGATCAAAG AGAAAGCCCT GAAATTCGTC GAGTATATAA ACTCCAAACT
     1741 CCCGGGCCTG CTGGAACTGG AATATGAAGG CTTCTACGCG CGCGGCTTCT TTGTGACCAA
     1801 AAAAAAATAC GCTCTGATTG ATGAGGAAGG TAAAATAGTT ACCCGCGGCC TGGAAATCGT
     1861 GCGCCGTGAC TGGAGCGAAA TCGCGAAAGA GACTCAGGCA AAAGTTCTGG AAGCAATCCT
     1921 GAAGCACGGC AACGTTGACG AGGCGGTGAA AATCGTAAAG GAAGTTACCG AAAAGCTGTC
     1981 GAAGTATGAG ATCCCACCAG AAAAACTGGT TATTTACGAG CAAATCACCC GCCCGTTATC
     2041 AGAATACAAA GCAATTGGCC CGCATGTAGC TGTGGCCAAA CGCCTGGCCG CGAAAGGCGT
     2101 AAAAGTTAAA CCGGGTATGG TAATTGGGTA CATCGTCCTG AAGGGAGATG GTCCAATCAG
     2161 CAAACGTGCC ATTGCTATTG AAGAGTTTGA TCCGAAAAAA CACAAATACG ACGCCGAATA
     2221 CTATATTGAA AACCAAGTTC TGCCGGCCGT TGAACGTATC CTGCGCGCGT TCGGCTACCG
     2281 TAAGGAGGAT CTGCGCTACC AAAAAACCAA ACAGGTGGGT CTGGGTGCTT GGCTGAAATT
     2341 CGGAGGTCGA GACCACCGGT TGTACCTATC GAGTGCCAGC TACAAAAACG TGGACGTAGG
     2401 GATAACAGGG TAATGGGCGA CGAGAGGACT GTATACGCTG AGGTGTCAAT CGTCGGAGCC
     2461 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     2521 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     2581 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     2641 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     2701 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     2761 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     2821 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     2881 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     2941 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     3001 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     3061 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     3121 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     3181 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     3241 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     3301 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     3361 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     3421 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     3481 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     3541 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     3601 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     3661 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     3721 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     3781 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     3841 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     3901 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     3961 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     4021 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     4081 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     4141 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     4201 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     4261 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     4321 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     4381 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAG
//