LOCUS       Exported     3491 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3491
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1528..1575
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3423..3439
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3440..3471
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1395
                     /label="CD_RNAseInhibitor"
                     /note=""
     primer_bind     complement(1580..1596)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1663..2251)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2442..3302)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3303..3407)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1431..1490
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
                     /label="FreeGenes Barcode"
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GAGTCTTGAC ATCCAGTGTG AGCAGCTGAG
       61 TGATGCCCGG TGGACAGAGC TCCTTCCTCT GATCCAGCAA TACGAAGTGG TCAGGCTGGA
      121 TGACTGTGGC CTCACTGAAG TGCGGTGCAA AGACATCAGC TCAGCAGTCC AAGCCAACCC
      181 TGCCCTGACA GAGCTCAGCC TTCGCACCAA TGAACTGGGT GATGGCGGTG TGGGTCTGGT
      241 GCTCCAGGGC CTGCAAAATC CCACTTGTAA GATCCAGAAG CTGAGCCTTC AGAACTGCGG
      301 CTTGACGGAG GCTGGCTGTG GGATTCTGCC TGGCATGCTG CGCTCTTTGT CTACCCTGCG
      361 TGAGCTACAT CTCAATGATA ACCCTATGGG GGACGCAGGC CTGAAGCTGC TCTGTGAAGG
      421 ACTTCAGGAC CCCCAGTGCC GTCTTGAGAA GCTTCAGTTG GAATACTGTA ACCTCACAGC
      481 TACCAGCTGC GAGCCCCTGG CTTCAGTGCT CAGGGTGAAA GCTGACTTTA AGGAGCTAGT
      541 ATTGAGCAAC AACGACCTCC ATGAACCTGG TGTCCGTATT CTGTGCCAGG GCCTGAAGGA
      601 CTCTGCCTGT CAACTGGAGT CACTCAAGCT GGAGAACTGT GGTATCACAG CAGCCAACTG
      661 CAAGGATCTG TGCGATGTTG TGGCCTCCAA AGCTTCGCTA CAGGAACTGG ACTTGAGCAG
      721 CAACAAGCTA GGTAATGCAG GCATTGCAGC ACTGTGCCCA GGACTGCTGC TTCCCAGCTG
      781 TAAGCTCAGG ACTCTGTGGC TCTGGGAATG TGATATCACT GCGGAGGGCT GCAAGGACCT
      841 GTGCCGTGTC CTCAGAGCCA AGCAGAGCCT GAAGGAACTC AGCCTTGCTT CCAATGAGCT
      901 GAAGGATGAG GGTGCCCGGC TGCTGTGTGA GAGCCTATTA GAGCCTGGCT GTCAGCTGGA
      961 GTCACTGTGG ATAAAGACCT GTAGCCTCAC AGCTGCCTCC TGTCCCTACT TCTGCTCCGT
     1021 GTTGACCAAA AGCCGTTCTC TGTTGGAGCT GCAAATGAGC AGCAACCCAC TGGGAGATGA
     1081 GGGAGTCCAG GAGCTTTGCA AGGCCCTGAG CCAGCCAGAT ACAGTGCTGC GTGAGCTTTG
     1141 GTTGGGAGAC TGTGATGTGA CAAATAGTGG TTGCAGCAGC CTTGCCAATG TCCTGCTGGC
     1201 CAACCGCAGC CTGAGGGAAC TGGACCTCAG TAACAACTGC ATGGGGGGCC CAGGTGTCCT
     1261 GCAACTGCTG GAGAGCCTCA AACAGCCCAG CTGCACCCTT CAGCAGCTCG TCCTGTATGA
     1321 CATTTACTGG ACAAATGAGG TGGAAGAACA GCTTCGGGCC CTGGAGGAGG AAAGGCCTTC
     1381 CCTGAGGATC ATTTCCGGAG GTCGAGACCA GCCATCCTCT TATCTCGTGG GGGATAGTCT
     1441 AAGCTTAATA CTAGGGATAA CAGGGTAATT TGGGCTCATA AGGCACTTAT CGCTGAGGTG
     1501 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     1561 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     1621 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     1681 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1741 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1801 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1861 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1921 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1981 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     2041 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     2101 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     2161 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     2221 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT
     2281 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     2341 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     2401 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     2461 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     2521 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     2581 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     2641 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     2701 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     2761 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     2821 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     2881 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     2941 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     3001 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     3061 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     3121 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     3181 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     3241 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     3301 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     3361 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT
     3421 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTTCAGG
     3481 TGGAGTGGGA G
//