LOCUS       Exported     3878 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3878
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1915..1962
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3810..3826
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3827..3858
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1782
                     /label="CD_BsuLF"
                     /note=""
     primer_bind     complement(1967..1983)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2050..2638)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2829..3689)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3690..3794)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1818..1877
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GGATCAGAGC CTGGAAGATA TTAATGTTAA
       61 AACCGTTACC GATGTGACCA GCGATATTCT GGTTAGCCCG AGCGCATTTG TTGTTGAGCA
      121 GATTGGTGAT AACTATCACG AAGAACCGAT TCTGGGTTTT AGCATTGTTA ATGAAACCGG
      181 TGCCTATTTC ATCCCGAAAG ATATTGCAGT TGAAAGCGAA GTGTTCAAAG AATGGGTTGA
      241 AAACGACGAG CAGAAAAAAT GGGTGTTTGA TAGCAAACGT GCAGTTGTTG CACTGCGTTG
      301 GCAGGGTATT GAACTGAAAG GTGCAGAATT TGATACCCTG CTGGCAGCCT ATATTATCAA
      361 TCCGGGTAAT AGCTATGATG ATGTTGCAAG CGTTGCCAAA GATTATGGTC TGCATATTGT
      421 TAGCAGTGAT GAAAGCGTTT ATGGCAAAGG TGCAAAACGT GCCGTTCCGA GCGAAGATGT
      481 TCTGAGCGAA CATCTGGGTC GTAAAGCACT GGCAATTCAG AGTCTGCGTG AAAAACTGGT
      541 TCAAGAACTG GAAAATAACG ATCAGCTGGA ACTGTTTGAA GAATTAGAAA TGCCGCTGGC
      601 ACTGATTCTG GGCGAAATGG AAAGCACCGG TGTTAAAGTT GATGTTGATC GTCTGAAACG
      661 TATGGGTGAA GAACTGGGTG CCAAACTGAA AGAATATGAA GAAAAAATCC ACGAGATTGC
      721 CGGTGAACCG TTTAATATCA ATAGCCCGAA ACAGCTGGGT GTGATCCTGT TTGAAAAAAT
      781 TGGTCTGCCG GTTGTGAAAA AAACCAAAAC CGGTTATAGC ACCAGCGCAG ATGTGCTGGA
      841 AAAATTAGCA GATAAACATG ACATCGTGGA CTACATTCTG CAGTATCGTC AGATTGGTAA
      901 ACTGCAGAGC ACCTATATTG AAGGTCTGCT GAAAGTTACC CGTCCGGATA GCCATAAAGT
      961 TCATACCCGT TTTAATCAGG CACTGACCCA GACCGGTCGT CTGAGCAGCA CCGATCCGAA
     1021 TCTGCAGAAT ATTCCGATTC GTCTGGAAGA AGGTCGTAAA ATTCGTCAGG CATTTGTGCC
     1081 GAGCGAAAAA GATTGGCTGA TTTTTGCAGC AGATTATAGC CAGATTGAAC TGCGTGTTCT
     1141 GGCACATATT AGCAAAGATG AAAATCTGAT CGAAGCCTTC ACCAACGATA TGGATATTCA
     1201 TACCAAAACA GCCATGGATG TGTTTCATGT TGCGAAAGAT GAAGTTACCA GCGCAATGCG
     1261 TCGTCAGGCC AAAGCAGTTA ATTTTGGTAT TGTGTATGGC ATCAGCGATT ATGGCCTGAG
     1321 CCAGAATCTG GGTATTACCC GTAAAGAAGC AGGCGCATTT ATTGATCGCT ATCTGGAAAG
     1381 TTTTCAGGGC GTTAAAGCAT ACATGGAAGA TAGCGTTCAA GAGGCAAAAC AGAAAGGTTA
     1441 TGTTACCACA CTGATGCATC GTCGTCGTTA TATTCCGGAA CTGACCAGCC GTAATTTCAA
     1501 TATTCGTAGC TTTGCAGAAC GTACCGCAAT GAATACCCCG ATTCAGGGTA GCGCAGCAGA
     1561 TATCATCAAA AAAGCAATGA TTGACATGGC AGCGAAGCTG AAAGAAAAAC AGCTGAAAGC
     1621 ACGTCTGCTG CTGCAAGTGC ATGATGAACT GATCTTTGAA GCACCGAAAG AAGAAATCGA
     1681 GATCCTGGAA AAGCTGGTTC CGGAAGTTAT GGAACATGCA CTGGCACTGG ATGTTCCGCT
     1741 GAAAGTGGAT TTTGCAAGCG GTCCGAGCTG GTATGATGCA AAAGGAGGTC GAGACCAGGT
     1801 ATATTTCTCC CCTCGGGCGT CTTGAAGCCC ACGACCCCTA GGGATAACAG GGTAATGTGA
     1861 AGGGCATCCC CTTTCACCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1921 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1981 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     2041 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     2101 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     2161 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     2221 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     2281 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     2341 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     2401 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     2461 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     2521 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     2581 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     2641 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     2701 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     2761 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     2821 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     2881 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     2941 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     3001 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     3061 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     3121 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     3181 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     3241 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     3301 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     3361 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     3421 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     3481 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     3541 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     3601 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     3661 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     3721 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     3781 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     3841 GACCGGAGGC TTTTGACTTG GTTCAGGTGG AGTGGGAG
//