LOCUS       Exported     3296 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3296
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1333..1380
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3228..3244
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3245..3276
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1200
                     /label="CD_UvsX"
                     /note=""
     primer_bind     complement(1385..1401)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1468..2056)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2247..3107)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3108..3212)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1236..1295
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GAGCGATCTG AAAAGCCGTC TGATTAAAGC
       61 AAGCACCAGC AAACTGACCG CAGAACTGAC AGCAAGCAAA TTTTTCAACG AAAAAGATGT
      121 GGTGCGCACG AAAATTCCGA TGATGAATAT TGCACTGAGC GGTGAAATTA CCGGTGGTAT
      181 GCAGAGCGGT CTGCTGATTC TGGCTGGTCC GAGCAAAAGC TTTAAAAGCA ATTTTGGTCT
      241 GACCATGGTG AGCAGCTATA TGCGTCAGTA TCCGGATGCA GTTTGTCTGT TTTATGATAG
      301 CGAATTTGGT ATCACACCGG CATATCTGCG TAGCATGGGT GTTGATCCGG AACGTGTTAT
      361 TCATACACCG GTTCAGAGCC TGGAACAGCT GCGTATTGAT ATGGTTAATC AGCTGGATGC
      421 AATTGAACGT GGTGAAAAAG TGGTGGTGTT TATTGATAGC CTGGGTAATC TGGCAAGCAA
      481 AAAAGAAACC GAAGATGCCC TGAATGAAAA AGTTGTTAGC GATATGACCC GTGCCAAAAC
      541 CATGAAAAGC CTGTTTCGTA TTGTTACCCC GTATTTCAGC ACCAAAAACA TTCCGTGTAT
      601 TGCCATCAAC CATACCTATG AAACCCAAGA GATGTTTAGC AAAACCGTTA TGGGTGGTGG
      661 CACCGGTCCG ATGTATAGCG CAGATACCGT TTTTATCATT GGCAAACGCC AGATTAAAGA
      721 TGGTAGCGAT CTGCAGGGTT ATCAGTTTGT TCTGAATGTT GAAAAAAGCC GCACCGTGAA
      781 AGAGAAAAGC AAATTCTTTA TCGACGTGAA ATTCGATGGT GGCATTGATC CGTATAGTGG
      841 TCTGCTGGAT ATGGCACTGG AACTGGGTTT TGTTGTTAAA CCGAAAAATG GTTGGTATGC
      901 CCGTGAATTT CTGGATGAAG AAACCGGTGA AATGATCCGT GAAGAAAAAA GCTGGCGTGC
      961 AAAAGATACC AATTGTACCA CCTTTTGGGG TCCGCTGTTC AAACATCAGC CGTTTCGTGA
     1021 TGCCATTAAA CGTGCATATC AGCTGGGTGC CATCGATAGC AATGAAATTG TTGAAGCCGA
     1081 AGTGGATGAA CTGATCAATA GCAAAGTGGA AAAATTCAAG AGTCCGGAAT CCAAAAGCAA
     1141 AAGCGCAGCA GATCTGGAAA CCGATCTGGA ACAACTGAGC GATATGGAAG AATTTAACGA
     1201 AGGAGGTCGA GACCAGTGCT ATATCTTCCT CCCGGGCCGC AACAATGTCA TTCGTATAGG
     1261 GATAACAGGG TAATTAGTAG GCGCAGCACC ATACTCGCTG AGGTGTCAAT CGTCGGAGCC
     1321 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     1381 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     1441 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1501 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1561 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1621 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1681 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1741 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1801 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1861 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1921 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1981 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     2041 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     2101 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     2161 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     2221 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     2281 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     2341 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     2401 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     2461 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     2521 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     2581 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     2641 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     2701 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     2761 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     2821 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     2881 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     2941 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     3001 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     3061 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     3121 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     3181 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     3241 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAG
//