LOCUS       Exported     4776 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4776
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2813..2860
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4708..4724
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4725..4756
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..2680
                     /label="CE_T7RNApol_Stop"
                     /note=""
     primer_bind     complement(2865..2881)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2948..3536)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3727..4587)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4588..4692)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    2716..2775
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GAACACTATC AACATTGCGA AGAACGATTT
       61 CTCTGACATC GAACTCGCGG CAATCCCGTT CAACACCCTG GCAGATCACT ACGGTGAACG
      121 TCTGGCCCGT GAACAGCTGG CTCTGGAACA CGAAAGCTAT GAAATGGGTG AGGCGCGTTT
      181 CCGTAAGATG TTCGAACGTC AGTTAAAAGC GGGTGAAGTT GCAGACAACG CCGCGGCGAA
      241 GCCGCTGATC ACCACCCTGC TGCCGAAAAT GATCGCTCGC ATCAACGATT GGTTCGAAGA
      301 AGTGAAAGCG AAACGTGGCA AACGTCCGAC TGCCTTCCAG TTCCTGCAAG AAATTAAACC
      361 GGAGGCAGTG GCGTATATCA CTATCAAAAC TACCCTGGCG TGCCTGACCT CTGCGGATAA
      421 CACGACCGTT CAGGCGGTAG CGTCCGCAAT CGGTCGTGCG ATCGAAGATG AAGCCCGCTT
      481 CGGCCGTATC CGTGACCTTG AAGCAAAACA CTTTAAAAAA AACGTTGAAG AACAGCTGAA
      541 CAAACGCGTT GGTCACGTAT ACAAAAAGGC GTTTATGCAG GTTGTGGAAG CTGATATGCT
      601 GTCCAAAGGC CTGCTGGGCG GCGAAGCGTG GAGCTCTTGG CATAAAGAAG ATTCTATTCA
      661 CGTGGGCGTT CGCTGCATTG AAATGCTGAT CGAATCGACT GGTATGGTTT CTCTGCACCG
      721 TCAGAACGCT GGTGTTGTTG GCCAGGATTC TGAAACTATC GAACTGGCCC CGGAATATGC
      781 GGAAGCGATC GCTACTCGCG CAGGTGCGCT GGCCGGTATT TCCCCGATGT TCCAGCCGTG
      841 CGTTGTACCG CCTAAACCGT GGACCGGCAT CACCGGCGGT GGTTATTGGG CTAACGGTCG
      901 CCGTCCGCTG GCACTCGTTC GTACCCACAG CAAAAAGGCA CTGATGCGTT ACGAAGATGT
      961 GTACATGCCG GAGGTTTACA AAGCCATCAA CATTGCTCAG AATACCGCCT GGAAGATTAA
     1021 TAAAAAAGTG CTCGCGGTGG CGAACGTTAT TACCAAGTGG AAACACTGTC CGGTGGAAGA
     1081 TATCCCGGCT ATCGAGCGCG AGGAACTGCC GATGAAACCG GAAGATATCG ATATGAACCC
     1141 GGAAGCTCTG ACCGCGTGGA AACGTGCTGC TGCTGCCGTA TATCGCAAAG ACAAGGCGCG
     1201 TAAGTCTCGC CGTATCTCTC TGGAGTTTAT GCTGGAACAG GCGAACAAAT TCGCCAACCA
     1261 CAAAGCCATC TGGTTCCCGT ACAACATGGA TTGGCGTGGC CGTGTCTATG CAGTTTCTAT
     1321 GTTCAACCCG CAAGGGAATG ATATGACCAA AGGCCTGCTG ACTCTGGCAA AAGGTAAGCC
     1381 GATCGGCAAA GAAGGTTATT ACTGGCTGAA AATCCATGGC GCGAATTGCG CGGGTGTAGA
     1441 TAAGGTACCG TTCCCGGAAC GTATTAAGTT CATTGAAGAG AACCACGAGA ATATCATGGC
     1501 ATGCGCGAAA AGCCCGCTGG AAAACACTTG GTGGGCTGAG CAGGACTCCC CGTTCTGCTT
     1561 CCTGGCGTTC TGCTTCGAGT ACGCAGGTGT TCAACATCAT GGCCTGAGCT ACAACTGCTC
     1621 CCTGCCTCTG GCATTCGACG GTTCTTGCAG CGGTATCCAG CACTTTTCCG CTATGTTACG
     1681 TGATGAAGTA GGTGGCCGTG CTGTCAATCT GCTGCCGTCC GAAACCGTTC AAGATATCTA
     1741 TGGTATCGTA GCGAAAAAGG TTAACGAGAT TCTGCAAGCA GATGCTATCA ACGGTACCGA
     1801 CAACGAGGTA GTTACCGTGA CCGATGAAAA CACCGGTGAA ATTTCCGAAA AAGTAAAACT
     1861 GGGGACCAAG GCACTGGCTG GCCAGTGGCT GGCCTACGGC GTGACTCGCA GCGTCACCAA
     1921 ACGTAGCGTG ATGACGCTCG CTTATGGCAG CAAAGAGTTT GGCTTTCGCC AGCAAGTTCT
     1981 GGAGGATACT ATCCAGCCAG CCATTGACTC GGGCAAGGGT CTGATGTTTA CCCAGCCGAA
     2041 CCAGGCCGCT GGATATATGG CAAAACTGAT CTGGGAATCT GTAAGCGTCA CCGTTGTGGC
     2101 AGCGGTTGAA GCTATGAATT GGTTGAAAAG CGCCGCGAAA CTGCTGGCAG CGGAAGTGAA
     2161 AGATAAAAAA ACTGGCGAAA TCCTGCGTAA ACGCTGCGCG GTTCACTGGG TCACTCCGGA
     2221 TGGCTTCCCA GTTTGGCAGG AATACAAAAA ACCTATCCAG ACCCGCCTGA ATCTGATGTT
     2281 CCTGGGCCAA TTCCGTCTGC AACCGACCAT CAACACCAAC AAAGACAGCG AAATTGACGC
     2341 CCACAAACAG GAGTCCGGCA TTGCCCCTAA CTTCGTTCAC TCTCAGGACG GCTCTCATCT
     2401 GCGCAAAACT GTCGTTTGGG CTCATGAAAA GTACGGCATC GAATCCTTTG CGCTGATCCA
     2461 CGACTCCTTC GGTACCATCC CGGCGGACGC GGCCAACCTG TTCAAAGCTG TTCGTGAAAC
     2521 TATGGTAGAC ACGTACGAAA GCTGCGACGT TCTGGCAGAC TTCTACGATC AGTTCGCTGA
     2581 CCAGCTGCAC GAAAGCCAGC TGGACAAGAT GCCAGCTCTG CCGGCTAAAG GTAATCTGAA
     2641 CCTGCGCGAC ATTCTGGAAT CTGACTTCGC TTTCGCCTAA TAAGCTTCGA GACCACACTC
     2701 GATAGGTACA ACCGGCCTAG CTGACTCATT AGGCTGTAGG GATAACAGGG TAATCGTGAC
     2761 TAGACGGTTC GGCGCCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA
     2821 ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT
     2881 GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG
     2941 CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
     3001 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
     3061 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
     3121 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
     3181 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
     3241 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
     3301 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
     3361 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
     3421 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
     3481 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
     3541 GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     3601 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     3661 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     3721 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     3781 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     3841 TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC
     3901 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     3961 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     4021 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     4081 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     4141 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     4201 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     4261 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     4321 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     4381 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     4441 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     4501 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     4561 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     4621 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     4681 AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA
     4741 CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAG
//