LOCUS Exported 3017 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3017 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 1054..1101 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2949..2965 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2966..2997 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..921 /label="CD_PBCV-1_ligase" /note="" primer_bind complement(1106..1122) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1189..1777) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1968..2828) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2829..2933) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 957..1016 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AACGATGATG CTCACTCTCG GGTCTCAAAT GGCAATCACA AAGCCATTGC TCGCCGCCAC 61 ACTTGAAAAT ATCGAGGACG TGCAATTTCC GTGTTTGGCA ACTCCTAAAA TAGATGGCAT 121 CAGGAGCGTG AAACAAACGC AAATGCTCTC AAGAACTTTC AAACCAATTA GAAACTCTGT 181 CATGAACCGC CTGCTTACCG AACTGCTGCC GGAGGGCAGT GATGGTGAAA TTAGCATCGA 241 AGGTGCCACC TTTCAGGACA CCACGAGTGC GGTTATGACG GGCCATAAAA TGTATAATGC 301 TAAGTTTTCG TATTATTGGT TTGATTATGT GACGGACGAC CCCCTGAAGA AATATATTGA 361 TCGCGTAGAG GATATGAAGA ACTATATCAC CGTTCATCCT CACATTCTGG AACACGCGCA 421 AGTAAAAATT ATTCCTCTGA TTCCAGTTGA AATCAACAAT ATTACTGAAC TATTACAATA 481 TGAACGAGAT GTTTTGTCTA AAGGGTTCGA AGGAGTGATG ATACGAAAAC CGGACGGAAA 541 ATACAAGTTC GGTCGTTCTA CCTTGAAAGA AGGTATTCTA CTGAAGATGA AACAGTTCAA 601 AGATGCGGAG GCGACTATCA TCAGCATGAC GGCGCTGTTT AAAAACACAA ACACCAAAAC 661 GAAAGATAAT TTTGGGTACA GTAAACGTTC CACCCATAAG AGTGGCAAAG TAGAGGAGGA 721 CGTGATGGGG AGCATCGAAG TCGATTACGA CGGCGTTGTG TTCTCGATCG GCACCGGCTT 781 TGATGCTGAT CAGCGGCGCG ATTTTTGGCA GAACAAAGAA TCATACATTG GTAAAATGGT 841 GAAGTTCAAA TACTTCGAAA TGGGCTCCAA GGATTGCCCG CGCTTTCCGG TCTTTATTGG 901 TATTCGTCAC GAGGAGGACC GTGGAGGTCG AGACCACTTG AAATCTTGTC GCACGGTGGG 961 CGTTATGGAA CAGTCAATAG GGATAACAGG GTAATAGGAT CTATCTCCAG CACTCTCGCT 1021 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA 1081 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG 1141 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT 1201 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 1261 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 1321 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 1381 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 1441 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 1501 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 1561 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 1621 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 1681 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 1741 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC 1801 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 1861 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 1921 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 1981 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 2041 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 2101 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 2161 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 2221 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 2281 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 2341 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 2401 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 2461 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 2521 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 2581 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 2641 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 2701 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 2761 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 2821 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 2881 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC 2941 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG 3001 TTCAGGTGGA GTGGGAG //