LOCUS       Exported     3047 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3047
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1084..1131
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2979..2995
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2996..3027
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..951
                     /label="CD_HRP"
                     /note=""
     primer_bind     complement(1136..1152)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1219..1807)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1998..2858)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2859..2963)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    987..1046
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAGAATTACT GACCCCTCGG GGTCTCAAAT GCTAACGCCC ACTTTCTATG ATAACTCATG
       61 TCCAGATGTG AGTAATATTG TTCGCGATAC CATTGTCAAC GAGCTCCGCT CTGATCCGCG
      121 TATTGCCGCC TCGATATTGC GTTTGCACTT TCATGACTGC TTTGTCAATG GTTGTGATGC
      181 GAGCATCCTG TTGGACAGCA CAACCAGTTT TCGTACGGAA AAAGACGCGT TTGGTAACGC
      241 AAACTCCGCG AGAGGCTTTC CGGTGATTGA TAGGATGAAA GCGGCGGTGG AATCAGCATG
      301 CCCGCGGACC GTGAGCTGTG CCGATTTACT GACTATTGCG GCACAACAGT CGGTAACGTT
      361 AGCGGGCGGA CCGAGCTGGC GCGTTCCGTT GGGTCGCCGT GACTCACTGC AGGCTTTTCT
      421 GGATCTGGCT AACGCCAACC TGCCCGCACC ATTTTTTACC CTCCCACAGC TGAAGGATTC
      481 TTTTCGGAAC GTAGGCTTAA ATCGTTCCTC TGATCTGGTA GCACTGTCCG GGGGCCATAC
      541 CTTCGGAAAA AATCAATGTC GCTTTATCAT GGACCGTCTG TACAACTTTT CCAACACCGG
      601 CTTACCGGAC CCTACACTGA ATACCACTTA TCTGCAAACG CTTCGTGGCC TTTGCCCTCT
      661 CAATGGTAAC TTAAGTGCCC TGGTTGATTT CGATCTTCGT ACCCCTACGA TCTTCGACAA
      721 TAAATATTAC GTTAATCTTG AGGAACAGAA AGGCCTGATT CAGTCGGACC AGGAACTGTT
      781 CTCTAGCCCG GATGCTACCG ATACAATCCC GCTGGTGCGA AGTTTCGCTG ATTCGACGCA
      841 GACGTTCTTC AACGCGTTTG TCGAAGCCAT GGACCGCATG GGGAATATTA CTCCGCTGAC
      901 AGGGACCCAA GGTCAGATCC GCTTGAATTG CCGCGTGGTC AACAGCAATA GCGGAGGTCG
      961 AGACCAGTAA TGGCTCGGAT CGTCTTGCTG TACAGAAGTT CGGTCTATAG GGATAACAGG
     1021 GTAATACTCG TGTTTGTTAC GCACCACGCT GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA
     1081 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT
     1141 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT
     1201 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     1261 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     1321 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     1381 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
     1441 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     1501 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     1561 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     1621 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
     1681 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     1741 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     1801 ATCTCAAGAA GGCCTACTAT TAGCAACAAC GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     1861 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
     1921 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     1981 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
     2041 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
     2101 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA
     2161 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
     2221 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
     2281 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
     2341 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
     2401 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
     2461 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
     2521 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
     2581 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
     2641 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
     2701 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
     2761 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
     2821 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
     2881 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     2941 AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA
     3001 AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TTCAGGTGGA GTGGGAG
//