LOCUS       Exported     3705 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3705
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1742..1789
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3637..3653
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3654..3685
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1609
                     /label="N5E_CBD"
                     /note=""
     primer_bind     complement(1794..1810)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1877..2465)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2656..3516)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3517..3621)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1645..1704
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAGAATTACT GACCCCTCGG GGTCTCACGG CAAGATCGAA GAAGGGAAGT TAGTGATCGG
       61 ATCGTTAGAA GGCTGCTTTG CGAAAGGAAC CAATGTCCTG ATGGCGGACG GTAGCATTGA
      121 ATGTATCGAA AACATCGAAG TCGGAAACAA GGTGATGGGG AAAGATGGTC GTCCGCGTGA
      181 AGTTATCAAA CTGCCCCGTG GACGCGAAAC TATGTACTCA GTTGTCCAAA AGTCCCAGCA
      241 CCGTGCGCAC AAAAGTGATT CGTCGCGTGA AGTGCCCGAG CTGTTGAAGT TCACCTGTAA
      301 CGCAACGCAC GAACTGGTAG TGCGTACACC TCGCTCTGTG CGTCGTCTGT CTCGTACCAT
      361 TAAGGGTGTC GAGTATTTCG AGGTGATCAC TTTCGAAATG GGTCAGAAGA AGGCACCCGA
      421 CGGTCGTATT GTGGAACTGG TGAAAGAGGT GTCAAAGTCA TACCCGATCA GCGAAGGTCC
      481 GGAACGTGCC AATGAGTTAG TTGAATCGTA CCGCAAAGCT TCTAACAAAG CATACTTCGA
      541 ATGGACCATC GAAGCGCGTG ACCTGTCTCT GCTGGGGAGC CATGTGCGTA AAGCAACCTA
      601 TCAGACCTAC GCTCCGATCC TGTACGAGAA CGACCACTTC TTTGATTACA TGCAGAAATC
      661 GAAATTCCAC CTGACCATCG AGGGGCCAAA AGTCCTGGCC TACTTACTGG GTCTTTGGAT
      721 TGGTGATGGT CTTAGCGATC GTGCAACCTT CTCAGTAGAT AGTCGTGATA CCTCTCTGAT
      781 GGAACGTGTG ACTGAGTATG CGGAAAAGCT GAATCTGTGT GCCGAGTACA AGGACCGTAA
      841 GGAACCGCAA GTCGCGAAAA CCGTTAACCT GTATTCAAAA GTTGTTCGTG GTGCATCTAC
      901 GAACCCGGGA GTTAGTGCGT GGCAAGTCAA CACCGCTTAT ACGGCTGGCC AACTGGTAAC
      961 CTATAACGGG AAAACGTACA AGTGCCTGCA ACCGCACACC TCCTTGGCGG GATGGGAGCC
     1021 GAGCAACGTG CCAGCGCTGT GGCAATTGCA GGGCGGTCAC GGCGGTATCC GCAACAATTT
     1081 GAATACGGAA AACCCCTTGT GGGACGCGAT CGTGGGCCTG GGTTTCCTGA AAGACGGGGT
     1141 CAAGAACATC CCATCCTTCC TGAGCACCGA TAACATTGGT ACGCGTGAGA CATTCCTGGC
     1201 AGGATTGATT GATTCGGATG GCTATGTGAC TGACGAACAC GGTATTAAGG CTACTATCAA
     1261 AACCATTCAT ACCTCTGTTC GCGATGGGCT GGTTTCTTTA GCTCGCTCGC TGGGTTTAGT
     1321 GGTGAGCGTT AACGCGGAAC CGGCAAAAGT CGATATGAAT GTGACCAAAC ACAAGATCTC
     1381 TTATGCGATT TACATGTCTG GCGGAGATGT TCTGCTTAAC GTTCTGTCTA AATGTGCGGG
     1441 TAGCAAGAAA TTCCGTCCTG CGCCAGCGGC AGCTTTTGCG CGTGAATGTC GTGGTTTCTA
     1501 CTTCGAATTG CAAGAATTGA AAGAGGACGA CTATTACGGC ATTACCCTGT CCGACGACAG
     1561 CGATCATCAA TTCCTGTTGG GTTCTCAAGT GGTCGTACAA AACTAATGAT AGGCTTCGAG
     1621 ACCAGGCTCG ATACAGTTTC ACGTTAGACA ATCGGAAATG ATTTGTAGGG ATAACAGGGT
     1681 AATGAAAAGA TTCCTCAGTG GACTCGCTGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA
     1741 ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG
     1801 CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC
     1861 AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC
     1921 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
     1981 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT
     2041 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG
     2101 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
     2161 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC
     2221 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT
     2281 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC
     2341 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA
     2401 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
     2461 CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
     2521 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
     2581 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
     2641 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
     2701 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
     2761 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA
     2821 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
     2881 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
     2941 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
     3001 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
     3061 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
     3121 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
     3181 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
     3241 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
     3301 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
     3361 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
     3421 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
     3481 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
     3541 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     3601 TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA
     3661 AGCCTCCGAC CGGAGGCTTT TGACTTGGTT CAGGTGGAGT GGGAG
//