LOCUS Exported 3231 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3231 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1268..1315 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 3163..3179 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3180..3211 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 29..1135 /label="N5E_MBP" /note="" primer_bind complement(1320..1336) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(1403..1991) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2182..3042) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(3043..3147) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 1171..1230 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAGAATTACT GACCCCTCGG GGTCTCACGG CAAAATCGAA GAAGGTAAAC TGGTAATCTG 61 GATTAACGGC GATAAAGGCT ATAACGGCCT CGCTGAAGTC GGTAAGAAAT TCGAGAAAGA 121 TACCGGAATT AAAGTCACCG TTGAGCATCC GGATAAACTG GAAGAGAAAT TCCCACAGGT 181 TGCGGCAACT GGCGATGGCC CTGACATTAT CTTCTGGGCA CACGACCGCT TTGGTGGCTA 241 CGCTCAATCT GGCCTGTTGG CTGAAATCAC CCCGGACAAA GCGTTCCAGG ACAAGCTGTA 301 TCCGTTTACC TGGGATGCCG TACGTTACAA CGGCAAGCTG ATTGCTTACC CGATCGCTGT 361 TGAAGCGTTA TCGCTGATTT ATAACAAAGA CCTGCTGCCG AACCCGCCAA AAACCTGGGA 421 AGAGATCCCG GCGCTGGATA AAGAACTGAA AGCGAAAGGT AAGAGCGCGC TGATGTTCAA 481 CCTGCAAGAA CCGTACTTCA CCTGGCCGCT GATTGCTGCT GACGGGGGTT ATGCGTTCAA 541 GTATGAAAAC GGCAAGTACG ACATTAAAGA CGTGGGCGTG GATAACGCTG GCGCGAAAGC 601 GGGTCTGACC TTCCTGGTTG ACCTGATTAA AAACAAACAC ATGAATGCAG ACACCGATTA 661 CTCCATCGCA GAAGCTGCCT TTAATAAAGG CGAAACAGCG ATGACCATCA ACGGCCCGTG 721 GGCATGGTCC AACATCGACA CCAGCAAAGT GAATTATGGT GTAACGGTAC TGCCGACCTT 781 CAAGGGTCAA CCATCCAAAC CGTTCGTTGG CGTGCTGAGC GCAGGTATTA ACGCCGCCAG 841 TCCGAACAAA GAGCTGGCGA AAGAGTTCCT CGAAAACTAT CTGCTGACTG ATGAAGGTCT 901 GGAAGCGGTT AATAAAGACA AACCGCTGGG TGCCGTAGCG CTGAAGTCTT ACGAGGAAGA 961 GTTGGCGAAA GATCCACGTA TTGCCGCCAC GATGGAAAAC GCCCAGAAAG GTGAAATCAT 1021 GCCGAACATC CCGCAGATGT CCGCTTTCTG GTATGCCGTG CGTACTGCGG TGATCAACGC 1081 CGCCAGCGGT CGTCAGACTG TCGATGAAGC CCTGAAAGAC GCGCAGACTT AATGATAGGC 1141 TTCGAGACCA GGGTGCCTTG CTAATCTAGT GAAACGTGAA GTTCCGGTTC ATAGGGATAA 1201 CAGGGTAATA TCCTTTAGGA TGGTTCGCTG CGCTGAGGTG TCAATCGTCG GAGCCGCTGA 1261 GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG 1321 TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA 1381 GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 1441 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 1501 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 1561 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 1621 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 1681 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 1741 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 1801 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 1861 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 1921 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 1981 AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC 2041 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 2101 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 2161 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT 2221 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG 2281 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC 2341 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC 2401 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC 2461 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC 2521 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC 2581 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA 2641 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT 2701 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA 2761 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA 2821 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG 2881 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC 2941 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC 3001 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA 3061 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA 3121 GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA 3181 GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTTCAGG TGGAGTGGGA G //