LOCUS       Exported     2978 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2978
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1015..1062
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2910..2926
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2927..2958
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..883
                     /note="test to see if random mutagenesized enzyme will catalyze same reaction"
                     /label="Luz-Homologue"
     primer_bind     complement(1067..1083)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1150..1738)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1929..2789)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2790..2894)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    918..977
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCTAAT GGGAAATGCT GTTTCTTCTA AGGCTCAATC
       61 TTTTTTGACT AGAGCTTTTA CTTCTTTGCC TGGATCTGCT AGAGAAAGAA TGTTGGCTGT
      121 TGCTTGTACT GCTTGTATTG CTACTACTGC TTTTTTTTAT CCTGCTATTA GAAAGGATTA
      181 TCAGGCTTAT ATGTCTTTGG GACCTGGAGG ATTTCCTCAT AATGTTGTTG GATGGTTGGC
      241 TTCTTGTTTT TCTGGATTGT TTGGAAGAGA AACTACTTCT GTTGAATGTT ATGATGCTAT
      301 GCCTGAAAAG AGACAATGGT TGACTTCTCC TTTGCCTCAA AGAGTTGGAG AAAGACCTAA
      361 GACTGGAAAG CATTGTATTC CTCATAGACA AATTGATCAA TTTGTTTCTT CTGAATTTGC
      421 TCAATCTCAA TTGCATCCTA TTATTCCTAG AGTTCATGCT AAGTATTCTT CTATTACTAC
      481 TTGTAAGAGA TCCCAACATG AAAGAAGAAC TGATGCTTTG TTTTTGGCTC CTGATGTTCC
      541 TGCTCATAGA ACTGCTAGAA AGGCTTGGTT GGAAATTGCT CACATGCATG AAACTGCTGA
      601 ATATTCTTTG CATGTTGTTT TGGCTCCTAG AGATTGTATT GAAGTTATTT CTAAGGGATG
      661 GGGAGAAAGA CATCCTGTTT CTGGATTTGC TTTGCCTGTT GAATATTTGT TTATTTATAC
      721 TCCTAGAACT GAAGAAGAAA TTGCTGTTGT TGAAAGAATT ATTGAAGCTG CTGTTGGATT
      781 TATGGCTAAT TTGCCTTTGC CTGCTGGAGC TACTTCTGAA TTTGTTCAAA CTTCTATTCC
      841 TACTAAGGAT ACTGATGGAA CTGTTACTCC TGTTCCTCAG TGAAGAGCTT AGAGACCGCC
      901 ACGGCGAAAT TTAATGTAGA AATATCATTC CATGGACGTA GGGATAACAG GGTAATTATA
      961 TTTCAACCCT GCAAGGACGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1021 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1081 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1141 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1201 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1261 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1321 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1381 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1441 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1501 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1561 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1621 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1681 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1741 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     1801 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     1861 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     1921 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     1981 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     2041 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     2101 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     2161 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     2221 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     2281 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     2341 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     2401 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     2461 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     2521 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     2581 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     2641 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     2701 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     2761 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     2821 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     2881 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     2941 GACCGGAGGC TTTTGACTTG GTTCAGGTGG AGTGGGAG
//