LOCUS       Exported     2864 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2864
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      901..948
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2796..2812
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2813..2844
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..769
                     /label="Luz-Mc"
                     /note="Mycena citricolor fungal luciferase - found with bioinformatics. Not confirmed to function"
     primer_bind     complement(953..969)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1036..1624)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1815..2675)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2676..2780)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     misc_feature    804..863
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCTAAT GGCTTATCAA TTAACTTGGA TTCAAACTTT
       61 GGTTTTGGGA GCTTTGGTTG CTATGGCTGT TGCTTTTCCT TTTATTAAGA AGGATTATGA
      121 AACTTTTTTG AAGGGAGGAC CTTCTTATGC TCCTCAAAAT GTTAGAGGAT ATATTATTGT
      181 TTTGGTTTTG GCTTTGTTTA GACAAGAACA GTTGGGATTG GAAATTTATG ATAGAATGCC
      241 TGAAAAGAGA AGATGGTTGG CTAATTTGCC TCAAAGAGAA GGACCTAGAC CTAAGACTAC
      301 TTCTCATATT ATTCAAAGAC AGTTGTCTCA ACATACTGAT CCTGCTTTTG GAGCTGCTTA
      361 TTTGAAGGAT ACTGTTATTC CTAGAGTTCA AGCTAGACAT GCTGCTAATA CTCATATTGC
      421 TAGATCCACT TTTGAATTTC ATGCTGCTGC TATTTTTTTG AATGCTGATG TTCCTTTGCC
      481 TGAAGGATTG CCTGCTTCTG AAACTGTTAG AAGAACTAAG GGAGAAATTG CTCACATGCA
      541 TGATTATCAT GATTTTACTT TGCATTTGGC TTTGGCTGCT GCTGATGGAA AGGAAGTTGT
      601 TGGAAAGGGA TGGGGACAAA GACATCCTTT GGCTGGACCT GGAGTTCCTG GACCTCCTAA
      661 TGAATGGACT TTTGTTTATG CTCCTAGAAA TGAAGAAGAA ATGGGAGTTG TTGAACAAAT
      721 TGTTGAAGCT GCTATTGGAT ATATGTCTAA TGTTCCTGCT TTGGAGTGAA GAGCTTAGAG
      781 ACCCGTGAAT CTTGACCTGT GGTGCACTTA TGGTCGGGGT TGAGTAGGGA TAACAGGGTA
      841 ATGAGGTTAG CGTACACGTC TACCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
      901 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
      961 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
     1021 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     1081 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     1141 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     1201 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     1261 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     1321 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     1381 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     1441 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     1501 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     1561 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     1621 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     1681 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     1741 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     1801 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     1861 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     1921 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT
     1981 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     2041 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     2101 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     2161 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     2221 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     2281 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     2341 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     2401 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     2461 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     2521 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     2581 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     2641 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     2701 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     2761 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA
     2821 GCCTCCGACC GGAGGCTTTT GACTTGGTTC AGGTGGAGTG GGAG
//