LOCUS       Exported     2912 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2912
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      949..996
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2844..2860
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2861..2892
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..817
                     /label="Luz-Oo"
                     /note="Omphalotus olearius fungal luciferase - found with bioinformatics. Not confirmed to function."
     primer_bind     complement(1001..1017)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1084..1672)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1863..2723)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2724..2828)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    852..911
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCTAAT GGGTTTGCCT GCTTTTATTT ATAAGCCTAG
       61 ATTGGTTATT ACTTGTGTTT TTGTTTTGGC TTCTGCTTTG GCTTTTCCTT TTATTAGAAA
      121 GGATTATCAA ACTTTTTTGG AAGTTGGACC TTCTTATGCT CCTCAAAATT TGCAAGGATA
      181 TATTATTGTT TGTGTTTTGT CTTTGTTTAG ACAAGAACAA AAGGATGTTG CTATTTATGA
      241 TAGATTGCCT GAAAAGAGAA GATGGTTGGG AGATTTGCCT TTTAGAGAAG GACCTAGACC
      301 TTCTATTACT TCTCATATTA TTCAAAGACA AAGAACTCAG TTGGCTGATG CTGAATTTGC
      361 TACTAAGGAA TTGATTGGAA AGATTATTCC TAGAGTTCAA GCTAGACATA CTAATACTAC
      421 TTTTTTGTCT ACTTCTAAGT TTGAATTTCA TGCTCAAGCT ATTTTTTTGT TGCCTTCTAT
      481 TCCTATTAAT GATCCTCAAA ATATTCCTTC TCATGATACT GTTAGAAGAA CTAAGAGAGA
      541 AATTGCTCAC ATGCATGATT ATCATGATTG TACTTTGCAT TTGGCTTTGG CTGCTCAAGA
      601 TGGAAAGGAA GTTTTGGAAA AGGGATGGGG ACAAAGACAT CCTTTGGCTG GACCTGGAGT
      661 TCCTGGACCT CCTACTGAAT GGACTTTTTT GTATGCTCCC AGGTCTGAAG AAGAAGTTAG
      721 AGTTGTTGAA ATGATTGTTG AGGCTTCTGT TGTTTATATG ACTAATGATC CTGCTGATAA
      781 GATTGTTGAA GCTACTGTTC AAGGAACTGA AGAGTGAAGA GCTTAGAGAC CCATCCTTAT
      841 GCGATACGGG TATAACTGGT TGATTCATTT ATTAGGGATA ACAGGGTAAT TTGGATCGTC
      901 GGTATAATTC TCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
      961 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1021 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     1081 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1141 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1201 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1261 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1321 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1381 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1441 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     1501 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     1561 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     1621 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT
     1681 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     1741 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     1801 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     1861 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     1921 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     1981 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     2041 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     2101 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
     2161 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
     2221 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
     2281 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     2341 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     2401 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     2461 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     2521 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     2581 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     2641 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     2701 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     2761 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     2821 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG
     2881 AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG AG
//