LOCUS       Exported     3653 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3653
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1690..1737
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3585..3601
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3602..3633
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1558
                     /label="Luz-RFP"
                     /note="catalyzes light reaction of the luciferin. Fused to RFP, which should shift color to red."
     primer_bind     complement(1742..1758)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1825..2413)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2604..3464)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3465..3569)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1593..1652
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCTAAT GGGAAGAATA AACATCTCCC TTTCAAGCCT
       61 GTTCGAGAGG CTGTCTAAGC TCTCTTCCAG GAGCATAGCT ATCACATGTG GTGTGGTCCT
      121 TGCATCCGCC ATAGCTTTTC CAATAATCAG ACGAGATTAT CAGACATTCT TAGAGGTTGG
      181 ACCTTCTTAT GCTCCTCAGA ACTTTAGAGG GTACATCATC GTATGTGTTC TGTCACTGTT
      241 CAGGCAGGAG CAGAAAGGCT TAGCTATTTA TGATAGATTG CCTGAGAAGC GAAGATGGCT
      301 TGCTGACTTA CCTTTTAGAG AGGGTACAAG ACCTTCTATA ACTTCCCACA TCATCCAAAG
      361 ACAACGTACT CAACTCGTCG ATCAGGAGTT TGCTACCAGA GAACTCATCG ATAAGGTTAT
      421 CCCTAGAGTC CAGGCCAGAC ATACTGACAA AACCTTCCTG TCAACAAGTA AGTTTGAGTT
      481 CCACGCCAAA GCCATATTCC TGCTTCCTTC TATCCCAATT AACGATCCTC TTAATATCCC
      541 TTCACACGAC ACCGTTCGAC GAACCAAGAG AGAAATTGCA CACATGCACG ATTACCATGA
      601 TTGTACTCTG CACCTTGCAT TAGCTGCCCA AGATGGAAAA GAGGTGCTTA AGAAGGGCTG
      661 GGGTCAAAGA CATCCCTTAG CTGGCCCTGG CGTTCCAGGT CCTCCTACTG AGTGGACATT
      721 CTTGTATGCC CCAAGGAATG AAGAGGAAGC ACGTGTAGTG GAGATGATAG TTGAGGCTAG
      781 TATAGGTTAC ATGACCAATG ACCCAGCAGG AAAGATTGTC GAGAACGCTA AGGGATCTGG
      841 TGGTGGATCT GGTATGGCCA GTTCAGAGGA CGTAATCAAA GAATTTATGC GTTTCAAGGT
      901 TAGGATGGAG GGGAGCGTAA ATGGGCATGA GTTTGAAATA GAGGGCGAGG GAGAAGGAAG
      961 GCCTTACGAA GGCACCCAAA CCGCCAAGTT GAAAGTAACA AAAGGTGGAC CACTACCTTT
     1021 TGCATGGGAT ATTTTAAGTC CTCAGTTTCA ATACGGGAGC AAAGCATACG TGAAACACCC
     1081 CGCTGACATA CCCGACTATC TAAAATTATC TTTCCCTGAG GGCTTCAAAT GGGAGAGGGT
     1141 TATGAATTTC GAGGATGGAG GAGTGGTTAC CGTAACACAG GATTCATCCT TACAAGATGG
     1201 AGAGTTCATT TACAAAGTCA AGCTAAGGGG CACTAATTTT CCCTCTGACG GACCCGTCAT
     1261 GCAAAAGAAA ACTATGGGGT GGGAGGCATC CACAGAAAGG ATGTATCCAG AGGACGGAGC
     1321 CCTAAAGGGA GAAATAAAGA TGAGATTAAA GCTCAAAGAC GGCGGCCATT ACGACGCAGA
     1381 AGTAAAGACT ACATATATGG CCAAAAAGCC AGTCCAACTC CCCGGAGCCT ATAAAACTGA
     1441 TATAAAATTA GATATTACCA GCCACAATGA AGATTACACT ATTGTAGAGC AATACGAGCG
     1501 AGCAGAAGGG AGGCACAGTA CCGGCGCTGC TAGCCACCAC CACCACCACC ACGTGTGAAG
     1561 AGCTTAGAGA CCCTATCGGT GCATCATCTC GTACGGCGAA GGCGCAACAG TTATAGGGAT
     1621 AACAGGGTAA TCGATCGCGC ACTTACCGAG AACGCTGAGG TGTCAATCGT CGGAGCCGCT
     1681 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1741 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1801 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1861 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1921 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1981 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     2041 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     2101 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     2161 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     2221 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     2281 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     2341 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2401 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     2461 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     2521 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     2581 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2641 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2701 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     2761 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     2821 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     2881 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     2941 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     3001 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     3061 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     3121 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     3181 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     3241 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     3301 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3361 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3421 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3481 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     3541 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     3601 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTTCA GGTGGAGTGG GAG
//