LOCUS       Exported     2930 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2930
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      967..1014
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2862..2878
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2879..2910
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..835
                     /label="Luz-Y189F"
                     /note="catalyzes light reaction of the luciferin. Pocket mutation based off of firefly luciferase color-shifts"
     primer_bind     complement(1019..1035)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1102..1690)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1881..2741)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2742..2846)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    870..929
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCTAAT GGGTAGAATA AACATCTCCC TTTCAAGCCT
       61 GTTCGAGAGG CTGTCTAAGC TCTCTTCCAG GAGCATAGCT ATCACATGTG GTGTGGTCCT
      121 TGCATCCGCC ATAGCTTTTC CAATAATCAG ACGAGATTAT CAGACATTCT TAGAGGTTGG
      181 ACCTTCTTAT GCTCCTCAGA ACTTTAGAGG GTACATCATC GTATGTGTTC TGTCACTGTT
      241 CAGGCAGGAG CAGAAAGGCT TAGCTATTTA TGATAGATTG CCTGAGAAGC GAAGATGGCT
      301 TGCTGACTTA CCTTTTAGAG AGGGTACAAG ACCTTCTATA ACTTCCCACA TCATCCAAAG
      361 ACAACGTACT CAACTCGTCG ATCAGGAGTT TGCTACCAGA GAACTCATCG ATAAGGTTAT
      421 CCCTAGAGTC CAGGCCAGAC ATACTGACAA AACCTTCCTG TCAACAAGTA AGTTTGAGTT
      481 CCACGCCAAA GCCATATTCC TGCTTCCTTC TATCCCAATT AACGATCCTC TTAATATCCC
      541 TTCACACGAC ACCGTTCGAC GAACCAAGAG AGAAATTGCA CACATGCACG ATTTCCATGA
      601 TTGTACTCTG CACCTTGCAT TAGCTGCCCA AGATGGAAAA GAGGTGCTTA AGAAGGGCTG
      661 GGGTCAAAGA CATCCCTTAG CTGGCCCTGG CGTTCCAGGT CCTCCTACTG AGTGGACATT
      721 CTTGTATGCC CCAAGGAATG AAGAGGAAGC ACGTGTAGTG GAGATGATAG TTGAGGCTAG
      781 TATAGGTTAC ATGACCAATG ACCCAGCAGG AAAGATTGTC GAGAACGCTA AGTGAAGAGC
      841 TTAGAGACCG TGTTCTATGT CCCCATCGTC ACCAACGAAT CTTGTCCACC TAGGGATAAC
      901 AGGGTAATGC CGAGTCACAC CCTTTTTACC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
      961 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1021 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1081 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1141 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1201 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1261 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1321 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1381 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1441 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1501 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1561 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1621 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1681 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     1741 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     1801 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     1861 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     1921 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     1981 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     2041 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     2101 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     2161 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     2221 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     2281 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     2341 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     2401 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     2461 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     2521 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     2581 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     2641 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     2701 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     2761 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     2821 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     2881 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT GGAGTGGGAG
//