LOCUS Exported 2930 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2930 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 967..1014 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2862..2878 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2879..2910 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..835 /label="Luz-Y233F" /note="catalyzes light reaction of the luciferin. Pocket mutation based off of firefly luciferase color-shifts" primer_bind complement(1019..1035) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(1102..1690) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1881..2741) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2742..2846) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 870..929 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AACGATGATG CTCACTCTCG GGTCTCTAAT GGGTAGAATA AACATCTCCC TTTCAAGCCT 61 GTTCGAGAGG CTGTCTAAGC TCTCTTCCAG GAGCATAGCT ATCACATGTG GTGTGGTCCT 121 TGCATCCGCC ATAGCTTTTC CAATAATCAG ACGAGATTAT CAGACATTCT TAGAGGTTGG 181 ACCTTCTTAT GCTCCTCAGA ACTTTAGAGG GTACATCATC GTATGTGTTC TGTCACTGTT 241 CAGGCAGGAG CAGAAAGGCT TAGCTATTTA TGATAGATTG CCTGAGAAGC GAAGATGGCT 301 TGCTGACTTA CCTTTTAGAG AGGGTACAAG ACCTTCTATA ACTTCCCACA TCATCCAAAG 361 ACAACGTACT CAACTCGTCG ATCAGGAGTT TGCTACCAGA GAACTCATCG ATAAGGTTAT 421 CCCTAGAGTC CAGGCCAGAC ATACTGACAA AACCTTCCTG TCAACAAGTA AGTTTGAGTT 481 CCACGCCAAA GCCATATTCC TGCTTCCTTC TATCCCAATT AACGATCCTC TTAATATCCC 541 TTCACACGAC ACCGTTCGAC GAACCAAGAG AGAAATTGCA CACATGCACG ATTACCATGA 601 TTGTACTCTG CACCTTGCAT TAGCTGCCCA AGATGGAAAA GAGGTGCTTA AGAAGGGCTG 661 GGGTCAAAGA CATCCCTTAG CTGGCCCTGG CGTTCCAGGT CCTCCTACTG AGTGGACATT 721 CTTGTTTGCC CCAAGGAATG AAGAGGAAGC ACGTGTAGTG GAGATGATAG TTGAGGCTAG 781 TATAGGTTAC ATGACCAATG ACCCAGCAGG AAAGATTGTC GAGAACGCTA AGTGAAGAGC 841 TTAGAGACCG GTTACAATAA GGGCTCCGTA CATGGAAGGC TCAATAGATA TAGGGATAAC 901 AGGGTAATAA GCTTTAGTTA ATAGTAGAAC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG 961 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT 1021 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG 1081 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 1141 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 1201 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 1261 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 1321 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 1381 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 1441 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 1501 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 1561 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 1621 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 1681 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT 1741 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 1801 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT 1861 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC 1921 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG 1981 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT 2041 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA 2101 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG 2161 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG 2221 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC 2281 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG 2341 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG 2401 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG 2461 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT 2521 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG 2581 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA 2641 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA 2701 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT 2761 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 2821 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG 2881 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT GGAGTGGGAG //