LOCUS       Exported     3392 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3392
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1429..1476
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3324..3340
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3341..3372
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1297
                     /label="H3H"
                     /note="converts hispidin into luciferin. Required as second step in fungal bioluminescence pathway"
     primer_bind     complement(1481..1497)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1564..2152)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2343..3203)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3204..3308)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1332..1391
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCTAAT GGCATCGTTT GAGAATTCTC TAAGCGTTTT
       61 GATTGTCGGG GCCGGACTTG GTGGGCTTGC TGCTGCCATT GCTCTGCGTC GCCAAGGGCA
      121 TGTCGTGAAA ATATACGACT CCTCTAGCTT CAAAGCCGAA CTTGGTGCGG GACTCGCTGT
      181 GCCGCCTAAC ACCTTGCGCA GTCTACAGCA ACTTGGTTGC AATACCGAGA ACCTCAATGG
      241 TGTGGATAAT CTTTGCTTCA CTGCTATGGG GTATGACGGG AGTGTAGGGA TGATGAACAA
      301 CATGACTGAC TATCGAGAGG CATACGGTAC TTCTTGGATC ATGGTCCACC GCGTTGACTT
      361 GCATAACGAG CTGATGCGCG TAGCACTTGA TCCAGGTGGG CTCGGACCTC CTGCGACACT
      421 CCATCTTAAT CATCGTGTCA CATTCTGTGA TGTCGACGCT TGCACCGTGA CATTCACCAA
      481 CGGGACCACT CAATCAGCTG ATCTCATCGT TGGTGCAGAC GGTATACGCT CTACCATTCG
      541 GCGGTTTGTC TTAGAAGAGG ACGTGACTGT GCCTGCGTCA GGAATCGTCG GGTTTCGATG
      601 GCTTGTACAA GCTGACGCGC TGGACCCATA TCCTGAACTC GACTGGATTG TTAAAAAGCC
      661 TCCTCTAGGC GCGCGACTGA TCTCCACTCC TCAGAATCCA CAGTCTGGTG TTGGCTTGGC
      721 TGACAGGCGC ACTATCATCA TCTACGCATG TCGTGGCGGC ACCATGGTCA ATGTCCTTGC
      781 AGTGCATGAT GACGAACGTG ACCAGAACAC CGCAGATTGG AGTGTACCGG CTTCCAAAGA
      841 CGATCTATTT CGTGTTTTCC ACGATTACCA TCCACGCTTT CGGCGGCTTT TAGAGCTTGC
      901 GCAGGATATT AATCTCTGGC AAATGCGTGT TGTACCTGTT TTGAAAAAAT GGGTTAACAA
      961 GCGGGTTTGC TTGTTAGGAG ATGCTGCGCA CGCTTCTTTA CCGACGTTGG GTCAAGGTTT
     1021 TGGTATGGGT CTGGAAGATG CCGTAGCACT TGGTACACTC CTTCCAAAGG GTACCACTGC
     1081 ATCTCAGATC GAGACTCGAC TTGCGGTGTA CGAACAGCTA CGTAAGGATC GTGCGGAATT
     1141 TGTTGCGGCT GAATCATATG AAGAACAATA TGTTCCTGAA ATGCGGGGAC TTTATCTGAG
     1201 GTCAAAGGAA CTGCGTGATA GAGTCATGGG TTATGATATC AAAGTGGAGA GCGAGAAGGT
     1261 TCTCGAGACT CTCCTAAGAA GTTCTAATTC TGCCTGAAGA GCTTAGAGAC CCAGTCCTTG
     1321 CCAATCGAAG TGTGTACGTG CGAATACATT CCTAGGGATA ACAGGGTAAT TTATTATTGA
     1381 GGCTGGTTAC ACGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
     1441 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1501 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     1561 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1621 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1681 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1741 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1801 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1861 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1921 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     1981 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     2041 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     2101 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT
     2161 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     2221 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     2281 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     2341 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     2401 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     2461 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     2521 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     2581 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
     2641 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
     2701 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
     2761 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     2821 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     2881 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     2941 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     3001 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     3061 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     3121 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     3181 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     3241 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     3301 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG
     3361 AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG AG
//