LOCUS       Exported     5400 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..5400
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      3437..3484
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     5332..5348
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      5349..5380
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..3305
                     /label="HispS_1"
                     /note="converts caffeic acid to hispidin. Required as first step in fungal bioluminescence pathway. NOTE: this gene was split because it was too large. Both fragments are required for cloning"
     primer_bind     complement(3489..3505)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(3572..4160)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(4351..5211)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(5212..5316)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    3340..3399
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCTAAT GGGAAATAGC AGTAAGAATC CACCATCAAC
       61 CCTCCTCGAC GTGTTTCTCG ATACAGCCAG AAACTTGGAT ACCGCTCTGA GAAATGTCCT
      121 TGAATGCGGG GAGCATAGAT GGTCATATAG GGAACTCGAC ACCGTTAGCT CCGCTCTTGC
      181 TCAGCATTTG CGATATACCG TAGGGTTATC ACCAACAGTG GCTGTCATAT CTGAGAATCA
      241 TCCTTATATT TTGGCACTTA TGTTGGCCGT GTGGAAGTTA GGCGGCACTT TTGCTCCAAT
      301 CGATGTGCAC TCTCCTGCCG AATTAGTGGC TGGAATGCTG AACATCGTGT CACCATCATG
      361 TCTTGTCATA CCATCTAGCG ACGTAACAAA TCAGACCCTT GCCTGTGATT TGAACATACC
      421 CGTAGTTGCT TTCCATCCTC ATCAGTCTAC TATCCCAGAG CTGAATAAGA AGTACCTTAC
      481 AGACAGTCAG ATTTCACCTG ATCTTCCCTT CTCTGACCCA AACCGTCCAG CACTTTACTT
      541 ATTCACCAGC AGTGCCACAA GCAGAAGTAA TCTGAAATGC GTGCCATTGA CTCATACATT
      601 CATTCTTCGT AACAGCCTTT CTAAGAGGGC TTGGTGTAAA CGTATGCGAC CTGAGACTGA
      661 CTTTGATGGT ATCCGTGTCC TGGGCTGGGC TCCTTGGAGT CATGTGTTAG CCCACATGCA
      721 GGATATAGGG CCATTAACAC TCTTGAATGC TGGCTGCTAT GTTTTCGCCA CTACTCCATC
      781 AACTTATCCC ACTGAACTCA AAGACGATAG GGACTTGATT AGTTGCGCAG CAAACGCCAT
      841 TATGTACAAG GGTGTGAAAT CATTCGCATG TTTGCCCTTT GTTTTAGGAG GGTTAAAGGC
      901 CCTCTGTGAA AGCGAACCTA GTGTCAAGGC CCATCTCCAA GTTGAAGAGA GAGCACAGTT
      961 ATTGAAGTCA CTCCAACACA TGGAGATTTT AGAGTGCGGT GGAGCTATGT TAGAGGCTTC
     1021 TGTTGCCAGC TGGGCTATCG AAAACTGCAT ACCCATCAGC ATCGGTATAG GCATGACAGA
     1081 GACTGGCGGT GCATTGTTTG CAGGTCCCGT CCAAGCCATC AAAACAGGCT TTAGCTCCGA
     1141 GGATAAGTTT ATTGAGGATG CTACCTACCT CCTTGTTAAA GATGATCATG AATCACATGC
     1201 AGAGGAAGAT ATTAACGAAG GGGAACTTGT AGTGAAATCC AAAATGCTCC CTAGAGGGTA
     1261 CTTGGGCTAC TCTGACCCAT CATTTTCCGT TGATGACGCC GGATGGGTAA CTTTTCGTAC
     1321 AGGTGACCGT TACTCTGTGA CACCCGATGG TAAGTTTAGT TGGTTGGGTA GGAATACAGA
     1381 CTTCATACAG ATGACCTCCG GAGAGACACT TGATCCACGT CCTATCGAAA GCAGCCTGTG
     1441 TGAGTCATCC TTGATTTCTA GGGCTTGCGT AATCGGAGAC AAGTTCCTCA ATGGGCCCGC
     1501 AGCTGCCGTG TGCGCCATAA TCGAACTCGA ACCTACAGCT GTGGAGAAAG GGCAAGCCCA
     1561 CTCACGTGAG ATAGCAAGGG TGTTTGCTCC CATAAATAGG GATCTTCCAC CTCCATTGAG
     1621 AATCGCTTGG TCCCATGTAC TTGTGTTGCA GCCCTCTGAG AAGATTCCTA TGACAAAGAA
     1681 AGGCACTATT TTCAGAAAGA AAATCGAACA GGTATTCGGG AGCGCTCTTG GTGGTAGTTC
     1741 AGGAGACAAT AGCCAAGCCA CTGCCGATGC TGGTGTGGTA CGTAGGGATG AACTCAGTAA
     1801 TACTGTCAAG CACATTATAT CCAGAGTACT TGGCGTGTCT GACGACGAGC TTTTGTGGAC
     1861 ACTTAGCTTT GCCGAGTTAG GGATGACATC TGCCTTGGCC ACAAGAATCG CAAACGAGCT
     1921 TAACGAAGTA CTTGTAGGGG TTAATCTTCC CATTAACGCC TGTTATATTC ATGTCGATTT
     1981 ACCTAGCTTA AGCAATGCAG TCTACGCCAA GCTGGCACAC CTGAAACTCC CAGATCGAAC
     2041 ACCAGAGCCA CGTCAGGCTC CTGTCGAAAA CAGCGGAGGC AAGGAGATAG TCGTAGTAGG
     2101 CCAAGCATTT CGATTACCTG GAAGCATCAA TGATGTGGCA TCATTGCGTG ACGCCTTCCT
     2161 GGCCAGACAA GCCTCATCTA TTATTACCGA AATTCCATCT GATAGGTGGG ATCATGCTAG
     2221 TTTCTATCCA AAGGACATCA GGTTTAACAA GGCTGGGCTG GTTGACATTG CAAACTACGA
     2281 CCATAGCTTC TTTGGTCTGA CTGCTACCGA GGCATTGTAC TTGTCCCCTA CCATGAGACT
     2341 GGCACTTGAA GTATCTTTTG AAGCTCTCGA AAATGCAAAC ATCCCAGTGA GTCAGCTGAA
     2401 AGGATCTCAA ACAGCCGTCT ATGTCGCAAC TACTGATGAT GGGTTCGAAA CACTTTTGAA
     2461 TGCAGAAGCA GGCTATGACG CATATACTAG GTTCTATGGT ACTGGCAGGG CCGCCTCTAC
     2521 TGCTTCCGGC CGTATTTCTT GTCTGTTAGA TGTGCATGGC CCTAGTATTA CAGTCGATAC
     2581 CGCTTGCAGT GGTGGAGCCG TATGTATTGA CCAAGCCATT GATTATCTCC AAAGCTCATC
     2641 AGCCGCCGAC ACTGCCATCA TCTGCGCCAG TAACACTCAC TGTTGGCCAG GTTCCTTTAG
     2701 GTTTCTTTCT GCTCAGGGAA TGGTGAGTCC TGGTGGTAGA TGTGCCACTT TTACAACTGA
     2761 CGCTGATGGA TACGTACCTA GCGAGGGTGC CGTCGCATTT ATACTTAAAA CTCGTGAGGC
     2821 CGCAATGAGA GATAAAGACA CAATATTAGC CACCATAAAA GCCACTCAGA TAAGCCATAA
     2881 TGGAAGGTCC CAGGGACTTG TGGCACCTAA CGTGAACAGC CAGGCTGATC TTCACCGAAG
     2941 CCTCCTCCAG AAAGCTGGCC TTAGTCCCGC AGACATTAGG TTTATCGAGG CACACGGAAC
     3001 TGGTACTTCA TTAGGCGATC TGAGCGAGAT ACAGGCCATA AATGATGCTT ATACCAGTAG
     3061 TCAGCCACGA ACTACTGGAC CATTAATTGT GTCCGCAAGC AAGACAGTAA TCGGGCATAC
     3121 CGAGCCAGCT GGTCCTCTTG TTGGGATGTT GTCCGTGCTT AACAGCTTTA AGGAAGGCGC
     3181 AGTACCTGGG TTGGCTCATC TTACAGCTGA CAACTTAAAC CCCTCCCTGG ATTGCAGTTC
     3241 TGTACCATTA CTCATCCCAT ACCAACCTGT ACACTTAGCT GCACCCAAGC CACATCGAGC
     3301 CGCTGTCAGA TCTGAGACCG GCTCGATACA GTTTCACGTT GCTACCACAG TACTATGGTG
     3361 TAGGGATAAC AGGGTAATAT CTATACCCCG TGAAATACAC GCTGAGGTGT CAATCGTCGG
     3421 AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT
     3481 TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT
     3541 TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC
     3601 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC
     3661 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG
     3721 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA
     3781 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG
     3841 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC
     3901 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG
     3961 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT
     4021 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT
     4081 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC
     4141 GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC
     4201 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
     4261 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
     4321 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
     4381 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
     4441 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG
     4501 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
     4561 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
     4621 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
     4681 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
     4741 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
     4801 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
     4861 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
     4921 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
     4981 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
     5041 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
     5101 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
     5161 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
     5221 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     5281 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA
     5341 CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT GGAGTGGGAG
//