LOCUS       Exported     3882 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3882
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1919..1966
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3814..3830
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3831..3862
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1787
                     /label="HispS_2"
                     /note="converts caffeic acid to hispidin. Required as first step in fungal bioluminescence pathway. NOTE: this gene was split because it was too large. Both fragments are required for cloning"
     primer_bind     complement(1971..1987)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2054..2642)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2833..3693)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3694..3798)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1822..1881
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAGAT CTTATGGTTT CTCAGGAACT CTTGGAGGTA
       61 TCGTCCTGGA GGCTCCCGAC GAAGAGAGAC TTGAGGAAGA ATTGCCCAAT GACAAACCAA
      121 TGCTGTTTGT GGTTTCAGCT AAAACTCATA CAGCCCTCAT TGAGTACTTG GGCAGATACC
      181 TGGAGTTCTT GCTGCAAGCT AATCCACAAG ACTTTTGTGA CATCTGCTAT ACCAGTTGCG
      241 TCGGTAGAGA GCACTATAGG TATCGTTACG CATGTGTCGC TAATGACATG GAGGACTTAA
      301 TTGGACAACT GCAAAAGAGG CTCGGTAGCA AGGTTCCACC AAAGCCTTCT TACAAACGTG
      361 GCGCCCTCGC CTTCGCATTC TCAGGCCAGG GTACACAGTT TCGAGGCATG GCTACTGAGC
      421 TGGCTAAAGC CTATTCCGGT TTTCGAAAAA TCGTATCAGA CCTTGCTAAG CGTGCCTCTG
      481 AACTTTCAGG CCACGCCATT GATAGGTTCT TACTGGCATA CGACATTGGA GCCGAAAATG
      541 TAGCACCTGA CAGTGAAGCT GACCAAATCT GTATCTTTGT TTACCAGTGT TCAGTTTTAC
      601 GTTGGCTTCA AACTATGGGA ATCAGACCCT CTGCTGTTAT AGGACACTCC TTGGGAGAAA
      661 TCTCAGCTTC CGTCGCTGCT GGTGCTCTTT CACTCGACAG CGCACTCGAT CTTGTCATAT
      721 CAAGGGCCAG GTTATTAAGG AGTTCTGCAA GCGCCCCAGC TGGGATGGCT GCTATGTCTG
      781 CTTCCCAAGA TGAGGTGGTA GAATTGATTG GCAAACTCGA TCTGGATAAA GCCAATAGCC
      841 TGAGCGTGAG TGTTATCAAC GGTCCTCAAA ATACCGTTGT CAGCGGATCT TCCGCCGCTA
      901 TAGAGTCAAT AGTTGCTCTG GCCAAGGGGC GAAAGATAAA GGCTTCTGCT CTGAATATTA
      961 ACCAAGCATT CCACTCCCCT TATGTGGATT CTGCCGTTCC AGGACTGCGT GCATGGAGCG
     1021 AGAAACACAT TAGTTCTGCC AGACCTCTCC AAATTCCTCT CTATTCCACA TTACTGGGTG
     1081 CCCAGATTTC TGAGGGTGAA ATGTTAAATC CTGACCATTG GGTCGATCAC GCTAGGAAAC
     1141 CCGTTCAGTT CGCCCAGGCA GCTACCACAA TGAAGGAGTC ATTTACAGGT GTCATCATAG
     1201 ACATTGGGCC ACAAGTCGTA GCATGGTCAC TTTTGCTGTC TAATGGCCTT ACCTCTGTAA
     1261 CAGCTCTCGC TGCCAAGCGA GGAAGAAGCC AGCAGGTAGC CTTCTTATCT GCATTAGCTG
     1321 ATCTGTATCA GGATTATGGA GTCGTACCTG ATTTCGTGGG CTTGTATGCA CAGCAAGAGG
     1381 ACGCCTCAAG GCTGAAAAAG ACTGACATCT TAACCTATCC CTTCCAAAGA GGTGAGGAGA
     1441 CTTTATCATC CGGTAGCAGT ACTCCTACCC TTGAAAATAC AGACTTGGAC TCCGGCAAGG
     1501 AGCTGTTAAT GGGTCCCACA CGTGGGCTCT TGAGAGCTGA CGACCTTAGG GACAGCATAG
     1561 TCTCTTCCGT GAAAGATGTC CTCGAATTAA AGAGTAACGA GGATTTGGAC TTAAGCGAGA
     1621 GTCTTAATGC CTTGGGAATG GACTCCATCA TGTTTGCCCA GTTACGTAAG AGAATTGGTG
     1681 AAGGCTTGGG TCTGAACGTC CCTATGGTCT TTCTGAGTGA TGCATTCTCC ATTGGTGAGA
     1741 TGGTATCTAA CTTAGTAGAG CAGGCTGAGG CTTCTGAAGA CAACTGAAGA GCTTAGAGAC
     1801 CGCCATTGGA AAACTGAACG TTTACACTTG CTCCCAGTTA CGTAGGGATA ACAGGGTAAT
     1861 CGTACTAATT AGTCCATCGG CCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT
     1921 AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG
     1981 TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA
     2041 AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT
     2101 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA
     2161 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC
     2221 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT
     2281 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG
     2341 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
     2401 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT
     2461 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC
     2521 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG
     2581 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC
     2641 AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     2701 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
     2761 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
     2821 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
     2881 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
     2941 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT
     3001 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
     3061 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
     3121 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
     3181 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
     3241 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
     3301 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
     3361 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
     3421 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
     3481 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
     3541 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
     3601 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
     3661 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
     3721 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
     3781 ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC
     3841 CTCCGACCGG AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG AG
//