LOCUS       BBF10K_003492           5437 bp    DNA        linear       14-JUN-2022
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     terminator      1..2726
                     /note="transcription terminator for bacteriophage T7 RNA 
                     /locus_tag="T7 terminator"
                     /label="T7 terminator"
                     /ApEinfo_label="T7 terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      2727..5437
                     /note="transcription terminator for bacteriophage T7 RNA 
                     /locus_tag="T7 terminator(1)"
                     /label="T7 terminator(1)"
                     /ApEinfo_label="T7 terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      763..810
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
                     /locus_tag="T7 terminator(2)"
                     /label="T7 terminator(2)"
                     /ApEinfo_label="T7 terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      3489..3536
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
                     /locus_tag="T7 terminator(3)"
                     /label="T7 terminator(3)"
                     /ApEinfo_label="T7 terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2658..2674
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 for"
                     /label="M13 for"
                     /ApEinfo_label="M13 for"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     5369..5385
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 for(1)"
                     /label="M13 for(1)"
                     /ApEinfo_label="M13 for"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      2675..2706
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
                     /locus_tag="tonB terminator"
                     /label="tonB terminator"
                     /ApEinfo_label="tonB terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      5386..5417
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
                     /locus_tag="tonB terminator(1)"
                     /label="tonB terminator(1)"
                     /ApEinfo_label="tonB terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3541..3557)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 rev"
                     /label="M13 rev"
                     /ApEinfo_label="M13 rev"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(815..831)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 rev(1)"
                     /label="M13 rev(1)"
                     /ApEinfo_label="M13 rev"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(3624..4212)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(898..1486)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori(1)"
                     /label="ori(1)"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(4403..5248)
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="bla"
                     /label="bla"
                     /ApEinfo_label="bla"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(1677..2537)
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="bla(1)"
                     /label="bla(1)"
                     /ApEinfo_label="bla"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(5249..5353)
                     /note="ampicillin resistance gene promoter"
                     /locus_tag="bla promoter"
                     /label="bla promoter"
                     /ApEinfo_label="bla promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(2538..2642)
                     /note="ampicillin resistance gene promoter"
                     /locus_tag="bla promoter(1)"
                     /label="bla promoter(1)"
                     /ApEinfo_label="bla promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3392..3451
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked
                     by 21 base pair random sequences."
                     /locus_tag="FreeGenes Barcode"
                     /label="FreeGenes Barcode"
                     /ApEinfo_label="FreeGenes Barcode"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    666..725
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked
                     by 21 base pair random sequences."
                     /locus_tag="FreeGenes Barcode(1)"
                     /label="FreeGenes Barcode(1)"
                     /ApEinfo_label="FreeGenes Barcode"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTACCCAAT CCAACACTCG AATCCCCACC CGTTGGACCA
       61 AACCCGGCTC ATTAAGCGTC GGTTCAGATT TATTTCCTTT ATTTAAAAAA AAGGAAAGGG
      121 TAAAAAATAG AAAATTGGAA ACAGTTAAAG CCCAAAATTG TAATTTACCG AGAATTGTAA
      181 ATTTACCTGA AAACCCTACG CTATAGTTTC GACTATAAAT ACCAAACTTA GGACCTCACT
      241 TCAGAATCCC CTCGTCGCTG CGTCTCTCTC CCGCAACCTT CGATTTTCGT TTATTCGCAT
      301 CCATCGGAGA GAGAAAACAA TCAATAAGCG ACAATGGGAG ACCGTAACTA ACTTAAGTAA
      361 GGTCTCAGCT TCAAGAGCTC AGATATGAAG ATGAAGATGA AATATTTGGT GTGTCAAATA
      421 AAAAGCTTGT GTGCTTAAGT TTGTGTTTTT TTCTTGGCTT GTTGTGTTAT GAATTTGTGG
      481 CTTTTTCTAA TATTAAATGA ATGTAAGATC TCATTATAAT GAATAAACAA ATGTTTCTAT
      541 AATCCATTGT GAATGTTTTG TTGGATCTCT TCTGCAGCAT ATAACTACTG TATGTGCTAT
      601 GGTATGGACT ATGGAATATG ATTAAAGATA AGGATCAATG GATCCCAGTC CTTGCCAATC
      661 GAAGTCTCGT CCAGTATGTT AGTCGCTAGG GATAACAGGG TAATGAAGAT GGTTTCCTCT
      721 TCGTGCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
      781 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
      841 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
      901 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
      961 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1021 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1081 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1141 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1201 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1261 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     1321 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     1381 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     1441 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT
     1501 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     1561 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     1621 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     1681 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     1741 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     1801 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     1861 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     1921 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     1981 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     2041 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     2101 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     2161 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     2221 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     2281 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     2341 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     2401 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     2461 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     2521 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     2581 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     2641 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT
     2701 TTGACTTGGT TCAGGTGGAG TGGGAGAACG ATGATGCTCA CTCTCGGGTA CCCAATCCAA
     2761 CACTCGAATC CCCACCCGTT GGACCAAACC CGGCTCATTA AGCGTCGGTT CAGATTTATT
     2821 TCCTTTATTT AAAAAAAAGG AAAGGGTAAA AAATAGAAAA TTGGAAACAG TTAAAGCCCA
     2881 AAATTGTAAT TTACCGAGAA TTGTAAATTT ACCTGAAAAC CCTACGCTAT AGTTTCGACT
     2941 ATAAATACCA AACTTAGGAC CTCACTTCAG AATCCCCTCG TCGCTGCGTC TCTCTCCCGC
     3001 AACCTTCGAT TTTCGTTTAT TCGCATCCAT CGGAGAGAGA AAACAATCAA TAAGCGACAA
     3061 TGGGAGACCG TAACTAACTT AAGTAAGGTC TCAGCTTCAA GAGCTCAGAT ATGAAGATGA
     3121 AGATGAAATA TTTGGTGTGT CAAATAAAAA GCTTGTGTGC TTAAGTTTGT GTTTTTTTCT
     3181 TGGCTTGTTG TGTTATGAAT TTGTGGCTTT TTCTAATATT AAATGAATGT AAGATCTCAT
     3241 TATAATGAAT AAACAAATGT TTCTATAATC CATTGTGAAT GTTTTGTTGG ATCTCTTCTG
     3301 CAGCATATAA CTACTGTATG TGCTATGGTA TGGACTATGG AATATGATTA AAGATAAGGA
     3361 TCAATGGATC CCAGTCCTTG CCAATCGAAG TCTCGTCCAG TATGTTAGTC GCTAGGGATA
     3421 ACAGGGTAAT GAAGATGGTT TCCTCTTCGT GCGCTGAGGT GTCAATCGTC GGAGCCGCTG
     3481 AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG
     3541 GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG
     3601 AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
     3661 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
     3721 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
     3781 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
     3841 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
     3901 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
     3961 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
     4021 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC
     4081 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
     4141 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
     4201 AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT
     4261 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA
     4321 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT
     4381 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA
     4441 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC
     4501 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG
     4561 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG
     4621 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT
     4681 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT
     4741 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT
     4801 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA
     4861 AGTTGGCCGC AGTGTTATCA CTACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT
     4921 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC
     4981 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA
     5041 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT
     5101 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT
     5161 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA
     5221 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT
     5281 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA
     5341 TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG
     5401 ACCGGAGGCT TTTGACTTGG TTCAGGTGGA GTGGGAG  
//
