LOCUS       Exported     7043 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..7043
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     rep_origin      1..589
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             6013..6873
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        5908..6012
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     promoter        1031..1059
                     /label="tac promoter"
                     /note="tac promoter"
     CDS             1112..3604
                     /label="Taq"
                     /note="The pOpenTaq plasmid. Originally created by Gene And Cell Technologies"
     primer_bind     complement(3645..3661)
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
ORIGIN
        1 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
       61 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
      121 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
      181 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
      241 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
      301 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
      361 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
      421 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
      481 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT
      541 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA
      601 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC
      661 GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG
      721 CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT
      781 ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGAATTA ATTCTCATGT
      841 TTGACAGCTT ATCATCGACT GCACGGTGCA CCAATGCTTC TGGCGTCAGG CAGCCATCGG
      901 AAGCTGTGGT ATGGCTGTGC AGGTCGTAAA TCACTGCATA ATTCGTGTCG CTCAAGGCGC
      961 ACTCCCGTTC TGGATAATGT TTTTTGCGCC GACATCATAA CGGTTCTGGC AAATATTCTG
     1021 AAATGAGCTG TTGACAATTA ATCATCGGCT CGTATAATGT GTGGAATTGT GAGCGGATAA
     1081 CAATTTCACA CAGGAAACAG CGATGAATTC GGGGATGCTG CCCCTCTTTG AGCCCAAGGG
     1141 CCGGGTCCTC CTGGTGGACG GCCACCACCT GGCCTACCGC ACCTTCCACG CCCTGAAGGG
     1201 CCTCACCACC AGCCGGGGGG AGCCGGTGCA GGCGGTCTAC GGCTTCGCCA AGAGCCTCCT
     1261 CAAGGCCCTC AAGGAGGACG GGGACGCGGT GATCGTGGTC TTTGACGCCA AGGCCCCCTC
     1321 CTTCCGCCAC GAGGCCTACG GGGGGTACAA GGCGGGCCGG GCCCCCACGC CGGAGGACTT
     1381 TCCCCGGCAA CTCGCCCTCA TCAAGGAGCT GGTGGACCTC CTGGGGCTGG CGCGCCTCGA
     1441 GGTCCCGGGC TACGAGGCGG ACGACGTCCT GGCCAGCCTG GCCAAGAAGG CGGAAAAGGA
     1501 GGGCTACGAG GTCCGCATCC TCACCGCCGA CAAAGACCTT TACCAGCTCC TTTCCGACCG
     1561 CATCCACGTC CTCCACCCCG AGGGGTACCT CATCACCCCG GCCTGGCTTT GGGAAAAGTA
     1621 CGGCCTGAGG CCCGACCAGT GGGCCGACTA CCGGGCCCTG ACCGGGGACG AGTCCGACAA
     1681 CCTTCCCGGG GTCAAGGGCA TCGGGGAGAA GACGGCGAGG AAGCTTCTGG AGGAGTGGGG
     1741 GAGCCTGGAA GCCCTCCTCA AGAACCTGGA CCGGCTGAAG CCCGCCATCC GGGAGAAGAT
     1801 CCTGGCCCAC ATGGACGATC TGAAGCTCTC CTGGGACCTG GCCAAGGTGC GCACCGACCT
     1861 GCCCCTGGAG GTGGACTTCG CCAAAAGGCG GGAGCCCGAC CGGGAGAGGC TTAGGGCCTT
     1921 TCTGGAGAGG CTTGAGTTTG GCAGCCTCCT CCACGAGTTC GGCCTTCTGG AAAGCCCCAA
     1981 GGCCCTGGAG GAGGCCCCCT GGCCCCCGCC GGAAGGGGCC TTCGTGGGCT TTGTGCTTTC
     2041 CCGCAAGGAG CCCATGTGGG CCGATCTTCT GGCCCTGGCC GCCGCCAGGG GGGGCCGGGT
     2101 CCACCGGGCC CCCGAGCCTT ATAAAGCCCT CAGGGACCTG AAGGAGGCGC GGGGGCTTCT
     2161 CGCCAAAGAC CTGAGCGTTC TGGCCCTGAG GGAAGGCCTT GGCCTCCCGC CCGGCGACGA
     2221 CCCCATGCTC CTCGCCTACC TCCTGGACCC TTCCAACACC ACCCCCGAGG GGGTGGCCCG
     2281 GCGCTACGGC GGGGAGTGGA CGGAGGAGGC GGGGGAGCGG GCCGCCCTTT CCGAGAGGCT
     2341 CTTCGCCAAC CTGTGGGGGA GGCTTGAGGG GGAGGAGAGG CTCCTTTGGC TTTACCGGGA
     2401 GGTGGAGAGG CCCCTTTCCG CTGTCCTGGC CCACATGGAG GCCACGGGGG TGCGCCTGGA
     2461 CGTGGCCTAT CTCAGGGCCT TGTCCCTGGA GGTGGCCGAG GAGATCGCCC GCCTCGAGGC
     2521 CGAGGTCTTC CGCCTGGCCG GCCACCCCTT CAACCTCAAC TCCCGGGACC AGCTGGAAAG
     2581 GGTCCTCTTT GACGAGCTAG GGCTTCCCGC CATCGGCAAG ACGGAGAAGA CCGGCAAGCG
     2641 CTCCACCAGC GCCGCCGTCC TGGAGGCCCT CCGCGAGGCC CACCCCATCG TGGAGAAGAT
     2701 CCTGCAGTAC CGGGAGCTCA CCAAGCTGAA GAGCACCTAC ATTGACCCCT TGCCGGACCT
     2761 CATCCACCCC AGGACGGGCC GCCTCCACAC CCGCTTCAAC CAGACGGCCA CGGCCACGGG
     2821 CAGGCTAAGT AGCTCCGATC CCAACCTCCA GAACATCCCC GTCCGCACCC CGCTTGGGCA
     2881 GAGGATCCGC CGGGCCTTCA TCGCCGAGGA GGGGTGGCTA TTGGTGGCCC TGGACTATAG
     2941 CCAGATAGAG CTCAGGGTGC TGGCCCACCT CTCCGGCGAC GAGAACCTGA TCCGGGTCTT
     3001 CCAGGAGGGG CGGGACATCC ACACGGAGAC CGCCAGCTGG ATGTTCGGCG TCCCCCGGGA
     3061 GGCCGTGGAC CCCCTGATGC GCCGGGCGGC CAAGACCATC AACTTCGGGG TCCTCTACGG
     3121 CATGTCGGCC CACCGCCTCT CCCAGGAGCT AGCCATCCCT TACGAGGAGG CCCAGGCCTT
     3181 CATTGAGCGC TACTTTCAGA GCTTCCCCAA GGTGCGGGCC TGGATTGAGA AGACCCTGGA
     3241 GGAGGGCAGG AGGCGGGGGT ACGTGGAGAC CCTCTTCGGC CGCCGCCGCT ACGTGCCAGA
     3301 CCTAGAGGCC CGGGTGAAGA GCGTGCGGGA GGCGGCCGAG CGCATGGCCT TCAACATGCC
     3361 CGTCCAGGGC ACCGCCGCCG ACCTCATGAA GCTGGCTATG GTGAAGCTCT TCCCCAGGCT
     3421 GGAGGAAATG GGGGCCAGGA TGCTCCTTCA GGTCCACGAC GAGCTGGTCC TCGAGGCCCC
     3481 AAAAGAGAGG GCGGAGGCCG TGGCCCGGCT GGCCAAGGAG GTCATGGAGG GGGTGTATCC
     3541 CCTGGCCGTG CCCCTGGAGG TGGAGGTGGG GATAGGGGAG GACTGGCTCT CCGCCAAGGA
     3601 GTGATAGATC CTCTAGAGTC GACCTGCAGG CATGCAAGCT TGGCACTGGC CGTCGTTTTA
     3661 CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC
     3721 CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG
     3781 CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT
     3841 ATTTCACACC GCATAAATTC CCTGTTTTGG CGGATGAGAG AAGATTTTCA GCCTGATACA
     3901 GATTAAATCA GAACGCAGAA GCGGTCTGAT AAAACAGAAT TTGCCTGGCG GCAGTAGCGC
     3961 GGTGGTCCCA CCTGACCCCA TGCCGAACTC AGAAGTGAAA CGCCGTAGCG CCGATGGTAG
     4021 TGTGGGGTCT CCCCATGCGA GAGTAGGGAA CTGCCAGGCA TCAAATAAAA CGAAAGGCTC
     4081 AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA
     4141 GGACAAATCC GCCGGGAGCG GATTTGAACG TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG
     4201 CAGGACGCCC GCCATAAACT GCCAGGCATC AAATTAAGCA GAAGGCCATC CTGACGGATG
     4261 GCCTTTTTGC GTTTCTACAA ACTCTTCCTG TCGTCATATC TACAAGCCAT CCCCCCACAG
     4321 ATACGGTAAA CTAGCCTCGT TTTTGCATCA GGAAAGCAGG GAATTTATGG TGCACTCTCA
     4381 GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG
     4441 ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT
     4501 CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG
     4561 GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT
     4621 GAGGTTCTGT ACCCGACACC ATCGAATGGT GCAAAACCTT TCGCGGTATG GCATGATAGC
     4681 GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC AGTAACGTTA TACGATGTCG
     4741 CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG GCCAGCCACG
     4801 TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA
     4861 ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA
     4921 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC GCCGATCAAC
     4981 TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC TGTAAAGCGG
     5041 CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT CCGCTGGATG
     5101 ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG
     5161 TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG
     5221 GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG GGCCCATTAA
     5281 GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA ATATCTCACT CGCAATCAAA
     5341 TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT GTCCGGTTTT CAACAAACCA
     5401 TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG
     5461 CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GATATCTCGG
     5521 TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA ACCACCATCA
     5581 AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG CTTGCTGCAA CTCTCTCAGG
     5641 GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CCGTCTCACT GGTGAAAAGA AAAACCACCC
     5701 TGGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG
     5761 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTAAGTTAG
     5821 CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGACCTGCAA GAACCTCACG
     5881 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA
     5941 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA
     6001 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA
     6061 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT
     6121 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG
     6181 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC
     6241 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT
     6301 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA
     6361 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT
     6421 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT
     6481 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT
     6541 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA
     6601 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA
     6661 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT
     6721 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC
     6781 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT
     6841 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA
     6901 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT
     6961 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC
     7021 GTAGAAAAGA TCAAAGGATC TTC
//