LOCUS       Exported     6148 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..6148
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     CDS             100..6147
                     /label="pOpen_Yeast"
                     /note="A yeast expression vector, built for expressing Wolbachia genes. Has high copy E.coli origin, kanamycin resistance, and Gal1 promoter for yeast expression"
ORIGIN
        1 TACCCCACGT TCGGTCCACT GTGTGCCGAA CATGCTCCTT CACTATTTTA ACATGTGGAA
       61 TTAATTCTCA TGTTTGACAG CTTATCATCG AACTCTAAGA GGTGATACTT ATTTACTGTA
      121 AAACTGTGAC GATAAAACCG GAAGGAAGAA TAAGAAAACT CGAACTGATC TATAATGCCT
      181 ATTTTCTGTA AAGAGTTTAA GCTATGAAAG CCTCGGCATT TTGGCCGCTC CTAGGTAGTG
      241 CTTTTTTTCC AAGGACAAAA CAGTTTCTTT TTCTTGAGCA GGTTTTATGT TTCGGTAATC
      301 ATAAACAATA AATAAATTAT TTCATTTATG TTTAAAAATA AAAAATAAAA AAGTATTTTA
      361 AATTTTTAAA AAAGTTGATT ATAGCCGCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA
      421 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT
      481 TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA
      541 CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT
      601 AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT
      661 ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT
      721 GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG
      781 CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC
      841 GCCTGAGCGA GGCGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA
      901 TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT
      961 TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA
     1021 TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT
     1081 AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC
     1141 AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA
     1201 TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC
     1261 CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG
     1321 TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG
     1381 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT
     1441 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA
     1501 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC
     1561 TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC
     1621 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT
     1681 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG
     1741 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA
     1801 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT
     1861 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA
     1921 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC
     1981 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAG GTACCACGCC
     2041 TATTTTTATA GAGCTCTAGT ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG
     2101 GAAGACTCTC CTCCGTGCGT CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC
     2161 CTCGCGCCGC ACTGCTCCGA ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA
     2221 GAGGAAAAAT TGGCAGTAAC CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA
     2281 ACCATAGGAT GATAATGCGA TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA
     2341 AGCGATGATT TTTGATCTAT TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA
     2401 CTAATACTTT CAACATTTTC GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA
     2461 ACAAAAAATT GTTAATATAC CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATTCT
     2521 AGAAATGAGA GACCACTGGC TGTGTATAAG GGAGCCTGAC ATTTATATTC CCCAGAACAT
     2581 CAGGTTAATG GCGTTTTTGA TGTCATTTTC GCGGTGGCTG AGATCAGCCA CTTCTTCCCC
     2641 GATAACGGAG ACCGGCACAC TGGCCATATC GGTGGTCATC ATGCGCCAGC TTTCATCCCC
     2701 GATATGCACC ACCGGGTAAA GTTCACGGGA GACTTTATCT GACAGCAGAC GTGCACTGGC
     2761 CAGGGGGATC ACCATCCGTC GCCCGGGCGT GTCAATAATA TCACTCTGTA CATCCACAAA
     2821 CAGACGATAA CGGCTCTCTC TTTTATAGGT GTAAACCTTA AACTGCATTT CACCAGCCCC
     2881 TGTTCTCGTC AGCAAAAGAG CCGTTCATTT CAATAAACCG GGCGACCTCA GCCATCCCTT
     2941 CCTGATTTTC CGCTTTCCAG CGTTCGGCAC GCAGACGACG GGCTTCATTC TGCATGGTTG
     3001 TGCTTACCAG ACCGGAGATA TTGACATCAT ATATGCCTTG AGCAACTGAT AGCTGTCGCT
     3061 GTCAACTGTC ACTGTAATAC GCTGCTTCAT AGCATACCTC TTTTTGACAT ACTTCGGGTA
     3121 TACATATCAG TATATATTCT TATACCGCAA AAATCAGCGC GCAAATACGC ATACTGTTAT
     3181 CTGGCTTTTA GTAGGTCTCT GCTTCTGCAG GGCTCGAGTC ATGTAATTAG TTATGTCACG
     3241 CTTACATTCA CGCCCTCCCC CCACATCCGC TCTAACCGAA AAGGAAGGAG TTAGACAACC
     3301 TGAAGTCTAG GTCCCTATTT ATTTTTTTAT AGTTATGTTA GTATTAAGAA CGTTATTTAT
     3361 ATTTCAAATT TTTCTTTTTT TTCTGTACAG ACGCGTGTAC GCATGTAACA TTATACTGAA
     3421 AACCTTGCTT GAGAAGGTTT TGGGACGCTC GAAGGCTTTA ATTTCGATCA CGAGGCCCTT
     3481 TCGTCTTCAA GAATTAACTG TGGGAATACT CAGGTATCGT AAGATGCAAG AGTTCGAATC
     3541 TCTTAGCAAC CATTATTTTT TTCCTCAACA TAACGAGAAC ACACAGGGGC GCTATCGCAC
     3601 AGAATCAAAT TCGATGACTG GAAATTTTTT GTTAATTTCA GAGGTCGCCT GACGCATATA
     3661 CCTTTTTCAA CTGAAAAATT GGGAGAAAAA GGAAAGGTGA GAGGCCGGAA CCGGCTTTTC
     3721 ATATAGAATA GAGAAGCGTT CATGACTAAA TGCTTGCATC ACAATACTTG AAGTTGACAA
     3781 TATTATTTAA GGACCTATTG TTTTTTCCAA TAGGTGGTTA GCAATCGTCT TACTTTCTAA
     3841 CTTTTCTTAC CTTTTACATT TCAGCAATAT ATATATATAT TTCAAGGATA TACCATTCTA
     3901 ATGTCTGCCC CTATGTCTGC CCCTAAGAAG ATCGTCGTTT TGCCAGGTGA CCACGTTGGT
     3961 CAAGAAATCA CAGCCGAAGC CATTAAGGTT CTTAAAGCTA TTTCTGATGT TCGTTCCAAT
     4021 GTCAAGTTCG ATTTCGAAAA TCATTTAATT GGTGGTGCTG CTATCGATGC TACAGGTGTC
     4081 CCACTTCCAG ATGAGGCGCT GGAAGCCTCC AAGAAGGTTG ATGCCGTTTT GTTAGGTGCT
     4141 GTGGGTGGTC CTAAATGGGG TACAGGTAGT GTTAGACCTG AACAAGGTTT ACTAAAAATC
     4201 CGTAAAGAAC TTCAATTGTA CGCCAACTTA AGACCATGTA ACTTTGCATC CGACTCTCTT
     4261 TTAGACTTAT CTCCAATCAA GCCACAATTT GCTAAAGGTA CTGACTTCGT TGTTGTCAGA
     4321 GAATTAGTGG GAGGTATTTA CTTTGGTAAG AGAAAGGAAG ACGATGGTGA TGGTGTCGCT
     4381 TGGGATAGTG AACAATACAC CGTTCCAGAA GTGCAAAGAA TCACAAGAAT GGCCGCTTTC
     4441 ATGGCCCTAC AACATGAGCC ACCATTGCCT ATTTGGTCCT TGGATAAAGC TAATGTTTTG
     4501 GCCTCTTCAA GATTATGGAG AAAAACTGTG GAGGAAACCA TCAAGAACGA ATTTCCTACA
     4561 TTGAAGGTTC AACATCAATT GATTGATTCT GCCGCCATGA TCCTAGTTAA GAACCCAACC
     4621 CACCTAAATG GTATTATAAT CACCAGCAAC ATGTTTGGTG ATATCATCTC CGATGAAGCC
     4681 TCCGTTATCC CAGGTTCCTT GGGTTTGTTG CCATCTGCGT CCTTGGCCTC TTTGCCAGAC
     4741 AAGAACACCG CATTTGGTTT GTACGAACCA TGCCACGGTT CTGCTCCAGA TTTGCCAAAG
     4801 AATAAGGTTG ACCCTATCGC CACTATCTTG TCTGCTGCAA TGATGTTGAA ATTGTCATTG
     4861 AACTTGCCTG AAGAAGGTAA GGCCATTGAA GATGCAGTTA AAAAGGTTTT GGATGCAGGT
     4921 ATCAGAACTG GTGATTTAGG TGGTTCCAAC AGTACCACCG AAGTCGGTGA TGCTGTCGCC
     4981 GAAGAAGTTA AGAAAATCCT TGCTTAAAAA GATTCTCTTT TTTTATGATA TTTGTACATA
     5041 AACTTTATAA ATGAAATTCA TAATAGAAAC GACACGAAAT TACAAAATGG AATATGTTCA
     5101 TAGGGTAGAA TTAATTCTCA TGTTTGACAG CTTATCATCG ATCGTCCAAC TGCATGGAGA
     5161 TGAGTCGTGG CAAGAATACC AAGAGTTCCT CGGTTTGCCA GTTATTAAAA GACTCGTATT
     5221 TCCAAAAGAC TGCAACATAC TACTCAGTGC AGCTTCACAG AAACCTCATT CGTTTATTCC
     5281 CTTGTTTGAT TCAGAAGCAG GTGGGACAGG TGAACTTTTG GATTGGAACT CGATTTCTGA
     5341 CTGGGTTGGA AGGCAAGAGA GCCCCGAGAG CTTACATTTT ATGTTAGCTG GTGGACTGAC
     5401 GCCAGAAAAT GTTGGTGATG CGCTTAGATT AAATGGCGTT ATTGGTGTTG ATGTAAGCGG
     5461 AGGTGTGGAG ACAAATGGTG TAAAAGACTC TAACAAAATA GCAAATTTCG TCAAAAATGC
     5521 TAAGAAATAG GTTATTACTG AGTAGTATTT ATTTAAGTAT TGTTTGTGCA CTTGCCTGCA
     5581 AGCCTTTTGA AAAGCAAGCA TAAAAGATCT AAACATAAAA TCTGTAAAAT AACAAGATGT
     5641 AAAGATAATG CTAAATCATT TGGCTTTTTG ATTGATTGTA CAGGAAAATA TACATCGCAG
     5701 GGGGTTGACT TTTACCATTT CACCGCAATG GAATCAAACT TGTTGAAGAG AATGTTCACA
     5761 GGCGCATACG CTACAATGAC CCGATTCTTG CTAGCCTTTT CTCGGTCTTG CAAACAACCG
     5821 CCGGCAGCTT AGTATATAAA TACACATGTA CATACCTCTC TCCGTATCCT CGTAATCATT
     5881 TTCTTGTATT TATCGTCTTT TCGCTGTAAA AACTTTATCA CACTTATCTC AAATACACTT
     5941 ATTAACCGCT TTTACTATTA TCTTCTACGC TGACAGTAAT ATCAAACAGT GACACATATT
     6001 AAACACAGTG GTTTCTTTGC ATAAACACCA TCAGCCTCAA GTCGTCAAGT AAAGATTTCG
     6061 TGTTCATGCA GATAGATAAC AATCTATATG TTGATAATTA GCGTTGCCTC ATCAATGCGA
     6121 GATCCGTTTA ACCGGACCCT AGTGCACT
//