LOCUS       Exported     5950 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..5950
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      3829..3876
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     5882..5898
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      5899..5930
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     promoter        1635..1653
                     /label="T7 promoter"
                     /note="t7 promoter"
     CDS             100..5949
                     /label="OMMLV"
                     /note="This is an expression vector for MMLV-RT, inducible with IPTG and his-tagged"
     primer_bind     complement(4039..4055)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(4122..4710)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(4901..5761)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(5762..5866)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 TTAATTAACA TTACTCGCAT CCATTCTCAG GCTGTCTCGT CTCGTCTCAC GATAGTCATG
       61 CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGAGAT
      121 CCCGGTGCCT AATGAGTGAG CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC
      181 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC
      241 GGTTTGCGTA TTGGGCGCCA GGGTGGTTTT TCTTTTCACC AGTGACACGG GCAACAGCTG
      301 ATTGCCCTTC ACCGCCTGGC CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC
      361 CAGCAGGCGA AAATCCTGTT TGATGGTGGT TAACGGCGGG ATATAACATG AGCTATCTTC
      421 GGTATCGTCG TATCCCACTA CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT
      481 GGCGCGCATT GCGCCCAGCG CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT
      541 GCCCTCATTC AGCATTTGCA TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC
      601 CCGTTCCGCT ATCGGCTGAA TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG
      661 CAGACGCGCC GAGACAGAAC TTAATGGGCC CGCTAACAGC GCGATTTGCT GGTGACCCAA
      721 TGCGACCAGA TGCTCCACGC CCAGTCGCGT ACCATCTTCA TGGGAGAAAA TAATACTGTT
      781 GATGGGTGTC TGGTCAGAGA CATCAAGAAA TAACGCCGGA ACATTAGTGC AGGCAGCTTC
      841 CACAGCAATG GCATCCTGGT CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG
      901 CGCGAGAAGA TTGTGCACCG CCGCTTTACA GGCTTCGACG CCGCTTCGTT CTACCATCGA
      961 CACCACCACG CTGGCACCCA GTTGATCGGC GCGAGATTTA ATCGCCGCGA CAATTTGCGA
     1021 CGGCGCGTGC AGGGCCAGAC TGGAGGTGGC AACGCCAATC AGCAACGACT GTTTGCCCGC
     1081 CAGTTGTTGT GCCACGCGGT TGGGAATGTA ATTCAGCTCC GCCATCGCCG CTTCCACTTT
     1141 TTCCCGCGTT TTCGCAGAAA CGTGGCTGGC CTGGTTCACC ACGCGGGAAA CGGTCTGATA
     1201 AGAGACACCG GCATACTCTG CGACATCGTA TAACGTTACT GGTTTCACAT TCACCACCCT
     1261 GAATTGACTC TCTTCCGGGC GCTATCATGC CATACCGCGA AAGGTTTTGC ACCATTCGAT
     1321 GGTGTCCGGG ATCTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA
     1381 GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC
     1441 CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA
     1501 GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA
     1561 CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT
     1621 CGATCCCGCG AAATTAATAC GACTCACTAT AGGGGAATTG TGAGCGGATA ACAATTCCCC
     1681 TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC ATATGACTCT GAACATCGAA
     1741 GATGAACATC GTCTGCACGA AACCAGCAAA GAACCGGACG TTTCTCTGGG TTCAACCTGG
     1801 CTGTCTGATT TTCCGCAAGC TTGGGCCGAA ACTGGCGGTA TGGGCCTGGC CGTCCGTCAG
     1861 GCACCGCTGA TCATCCCACT GAAAGCGACC TCCACCCCGG TGTCCATCAA ACAGTATCCA
     1921 ATGTCCCAGG AAGCACGTCT GGGCATCAAG CCACATATCC AACGTCTGCT GGATGCCGGT
     1981 ATCCTGGTAC CGTGTCAATC GCCTTGGAAC ACTCCACTGC TGCCGGTGAA AAAACCGGGC
     2041 ACTAACGATT ACCGTCCGGT ACAGGACCTG CGTGAAGTTA ACAAACGTGT TGAAGATATT
     2101 CATCCGACCG TCCCGAATCC GTACAACCTC CTGTCCGGTC TGCCGCCGAG CCATCAGTGG
     2161 TACACCGTGC TGGACCTGAA GGACGCATTC TTTTGCCTGC GTCTGCACCC GACCTCTCAG
     2221 CCGCTGTTTG CGTTTGAATG GCGTGACCCG GAAATGGGTA TTTCTGGCCA GCTTACATGG
     2281 ACCCGCCTGC CACAGGGTTT CAAAAACTCC CCGACCCTTT TCGACGAAGC CCTGCACCGT
     2341 GACCTGGCAG ATTTTCGCAT TCAGCACCCG GACCTCATCC TGCTGCAATA CATGGACGAT
     2401 CTGCTGTTGG CAGCTACCAG CGAACTGGAT TGCCAGCAGG GTACCCGTGC GCTGCTGCAA
     2461 ACCCTGGGCA ACCTGGGTTA CCGTGCTTCC GCGAAAAAGG CGCAGATTTG CCAAAAACAG
     2521 GTCAAATATC TGGGTTACCT GCTGAAAGAA GGCCAGCGCT GGCTGACCGA GGCCCGCAAA
     2581 GAAACCGTGA TGGGTCAACC GACCCCGAAA ACCCCGCGTC AACTGCGCGA GTTTCTGGGT
     2641 ACCGCTGGCT TCTGCCGTCT GTGGATTCCG GGCTTCGCTG AAATGGCCGC GCCGCTGTAT
     2701 CCGTTAACCA AAACTGGTAC TCTGTTCAAC TGGGGTCCGG ACCAGCAGAA AGCCTATCAG
     2761 GAGATTAAAC AAGCGCTGCT GACCGCACCG GCGCTGGGCC TGCCGGACCT GACCAAACCA
     2821 TTTGAACTGT TCGTTGACGA GAAACAAGGT TACGCTAAAG GTGTTCTTAC CCAGAAACTG
     2881 GGTCCGTGGC GCCGTCCGGT AGCGTATCTG TCTAAAAAAC TGGACCCGGT TGCGGCTGGT
     2941 TGGCCGCCGT GTCTGCGTAT GGTTGCTGCT ATTGCCGTAC TGACTAAGGA TGCGGGCAAA
     3001 CTGACTATGG GCCAGCCGCT GGTGATCCTG GCTCCGCATG CGGTGGAGGC CCTGGTTAAA
     3061 CAGCCGCCGG ATCGCTGGCT GTCTAACGCG CGTATGACAC ACTACCAGGC TCTGCTGCTG
     3121 GATACCGACC GTGTTCAGTT CGGCCCGGTC GTGGCCCTGA ACCCGGCGAC CCTGCTCCCG
     3181 CTGCCGGAAG AAGGTCTGCA ACACAATTGC CTGGATATTC TGGCGGAAGC CCATGGTACT
     3241 CGTCCGGATC TGACCGACCA GCCTCTGCCG GATGCAGACC ACACCTGGTA CACTGGTGGC
     3301 AGCTCCCTCC TGCAAGAGGG CCAGCGTAAG GCAGGCGCGG CTGTTACCAC GGAAACCGAA
     3361 GTAATCTGGG CTAAAGCCCT GCCAGCAGGA ACGTCAGCCC AACGCGCACA ACTGATTGCT
     3421 CTGACCCAGG CACTGAAAAT GGCGGAAGGT AAAAAGCTGA ACGTTTACAC TAACTCCCGC
     3481 TACGCATTCG CTACCGCCCA CATCCATGGC GAAATCTACA GACGTCGTGG TCTGCTGACC
     3541 TCTGAAGGCA AAGAAATCAA AAACAAAGAT GAGATCCTGG CCCTGCTGAA AGCGCTGTTC
     3601 CTGCCTAAAC GTTTATCAAT CATCCACTGT CCGGGCCACC AGAAGGGCCA CTCCGCGGAA
     3661 GCGCGCGGCA ACCGTATGGC AGACCAGGCA GCACGCAAAG CTGCCAATTC GAGCTCCGTC
     3721 GACAAGCTTG CGGCCGCACT CGAGCACCAC CACCACCACC ACTGAGATCC GGCTGCTAAC
     3781 AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC
     3841 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA
     3901 TCCCCAGGAT ACATAGATTA CCACAACTCC GAGCCCTTCC ACCACTAGTC GCTGAGGTGT
     3961 CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT
     4021 TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA
     4081 TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC
     4141 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
     4201 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
     4261 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
     4321 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
     4381 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
     4441 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
     4501 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
     4561 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
     4621 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
     4681 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT
     4741 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG
     4801 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA
     4861 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG
     4921 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG
     4981 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC
     5041 GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG
     5101 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG
     5161 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG
     5221 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT
     5281 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC
     5341 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC
     5401 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA
     5461 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC
     5521 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT
     5581 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC
     5641 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA
     5701 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA
     5761 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT
     5821 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC
     5881 AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT
     5941 GGAGTGGGAG
//