LOCUS       Exported                6151 bp ds-DNA     circular SYN 24-SEP-2020
DEFINITION  synthetic linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 24, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..6151
                     /label=T7 terminator
                     /note="""transcription terminator for bacteriophage T7 RNA"
     promoter        1619..1637
                     /label=T7 promoter
                     /note="t7 promoter"
     CDS             1707..4040
                     /codon_start=1
                     /label=OpenVent
                     /translation="MIIDADYITEDGKPIIRIFKKEKGEFKVEYDRTFRPYIYALLKDD
                     SAIDEVKKITAERHGKIVRITEVEKVQKKFLGRPIEVWKLYLEHPQDVPAIREKIREHP
                     AVVDIFEYDIPFAKRYLIDKGLTPMEGNEELTFLAVDIETLYHEGEEFGKGPIIMISYA
                     DEEGAKVITWKSIDLPYVEVVSSEREMIKRLVKVIREKDPDVIITYNGDNFDFPYLLKR
                     AEKLGIKLPLGRDNSEPKMQRMGDSLAVEIKGRIHFDLFPAIRRTINLPTYTLETVYEV
                     IFGKSKEKVYAHEIAEAWETGKGLERVAKYSMEDAKVTSELGKEFFPMEAQLARLVGHP
                     VWDVSRSSTGNLVEWFLLTKAYERNELAPNKPDEREYERRLRESYEGGYVNEPEKGLWE
                     GIVSLDFRSLYPSIIITHNVSPDTLNRENCKEYDVAPQVGHRFCKDFPGFIPSLLGNLL
                     EERQKIKKRMKESKDPVEKKLLDYRQRAIKILANSYYGYYGYAKARWYCKECAESVTAW
                     GRQYIDLVRRELESRGFKVLYIDTDGLYATIPGAKHEEIKEKALKFVEYINSKLPGLLE
                     LEYEGFYARGFFVTKKKYALIDEEGKIVTRGLEIVRRDWSEIAKETQAKVLEAILKHGN
                     VDEAVKIVKEVTEKLSKYEIPPEKLVIYEQITRPLSEYKAIGPHVAVAKRLAAKGVKVK
                     PGMVIGYIVLKGDGPISKRAIAIEEFDPKKHKYDAEYYIENQVLPAVERILRAFGYRKE
                     DLRYQKTKQVGLGAWLKFHHHHHH"
     terminator      4055..4102
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(4240..4256)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(4323..4911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5102..5962)
                     /codon_start=1
                     /label=bla
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5963..6067)
                     /label=bla promoter
                     /note="ampicillin resistance gene promoter"
     primer_bind     6083..6099
                     /label=M13 for
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      6100..6131
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ttaattaaca ttactcgcat ccattctcag gctgtctcgt ctcgtctcgc gcccaccgga
       61 aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag
      121 tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      181 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
      241 gccagggtgg tttttctttt caccagtgac acgggcaaca gctgattgcc cttcaccgcc
      301 tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc
      361 tgtttgatgg tggttaacgg cgggatataa catgagctat cttcggtatc gtcgtatccc
      421 actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc
      481 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt
      541 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc
      601 tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca
      661 gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc
      721 acgcccagtc gcgtaccatc ttcatgggag aaaataatac tgttgatggg tgtctggtca
      781 gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc
      841 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc
      901 accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca
      961 cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc
     1021 agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg
     1081 cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca
     1141 gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac
     1201 tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc
     1261 gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc cgggatctcg
     1321 acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt
     1381 gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc
     1441 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
     1501 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     1561 ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta
     1621 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
     1681 tttaacttta agaaggagat atacatatga tcattgacgc tgactacatc actgaagatg
     1741 gcaaaccgat catccgtatt tttaaaaaag agaaaggtga attcaaagtc gaatacgacc
     1801 gcacctttcg cccgtacatt tacgcgctgc tgaaagatga cagcgctatt gatgaagtga
     1861 agaaaattac tgcggaacgt catggtaaaa ttgttcgtat tacggaggtg gaaaaagtcc
     1921 aaaaaaaatt cctgggccgt cctatcgagg tgtggaagct gtacctggaa catcctcagg
     1981 atgttccggc aattcgcgaa aaaatccgtg aacacccggc cgtcgtagat atcttcgagt
     2041 acgatatccc gttcgcaaaa cgttacctga ttgataaagg cctgacgccg atggagggca
     2101 acgaagaact gacctttctg gcggtggaca tcgaaactct gtatcacgaa ggcgaagaat
     2161 ttggtaaagg ccctattatc atgatttcgt atgcggatga ggaaggagcg aaagtgatca
     2221 catggaaaag cattgacctg ccgtacgttg aagtggtcag ctcagaacgc gaaatgatta
     2281 aacgcctcgt taaagtcatt cgtgaaaagg atccggacgt aatcatcacc tacaatggtg
     2341 ataacttcga ctttccgtat cttctgaaac gtgcggaaaa attgggcatt aaactgccgc
     2401 ttggtcgtga caacagcgaa ccgaaaatgc agcgcatggg tgacagcctg gcggtggaaa
     2461 ttaaaggtcg tatccacttc gacctgttcc cggcgatccg tcgtaccatc aacctgccga
     2521 cgtataccct ggaaacggtt tacgaagtta ttttcggcaa atccaaagaa aaagtttacg
     2581 ctcacgaaat cgcggaagca tgggaaaccg gtaaaggcct ggaacgtgtg gcaaagtaca
     2641 gcatggaaga tgcaaaagtg accagcgaac tggggaaaga attctttcca atggaagcgc
     2701 aactggcacg cctggtgggt catccggtct gggacgtgtc tcgtagctcg actggcaacc
     2761 tggtggaatg gttcctgctg actaaagctt acgaacgcaa cgagctcgca ccgaacaaac
     2821 cggatgaacg tgaatacgaa cgccgtctgc gtgagagcta cgaaggcggt tacgtgaacg
     2881 aaccggaaaa aggcctgtgg gagggtatcg tgagcctgga tttccgtagc ctgtacccgt
     2941 cgattatcat cactcataac gtatccccag atacgctcaa ccgtgaaaac tgtaaagaat
     3001 atgacgttgc gcctcaggtg ggtcatcgct tctgcaagga ttttccaggt ttcatcccga
     3061 gcctgctggg aaacctgctg gaggaacgtc aaaaaatcaa aaaacgcatg aaagagagta
     3121 aagacccggt ggaaaaaaag ctgctggatt accgtcagcg tgctattaag atccttgcga
     3181 actcttatta cggttattac ggctatgcaa aagcgcgttg gtactgcaaa gaatgcgctg
     3241 aatccgttac tgcatggggt cgtcagtaca tcgacctggt acgtagggaa ctggagagcc
     3301 gtggcttcaa agtactgtat atcgacactg atggtctgta cgccactatc ccgggcgcaa
     3361 aacatgaaga gatcaaagag aaagccctga aattcgtcga gtatataaac tccaaactcc
     3421 cgggcctgct ggaactggaa tatgaaggct tctacgcgcg cggcttcttt gtgaccaaaa
     3481 aaaaatacgc tctgattgat gaggaaggta aaatagttac ccgcggcctg gaaatcgtgc
     3541 gccgtgactg gagcgaaatc gcgaaagaga ctcaggcaaa agttctggaa gcaatcctga
     3601 agcacggcaa cgttgacgag gcggtgaaaa tcgtaaagga agttaccgaa aagctgtcga
     3661 agtatgagat cccaccagaa aaactggtta tttacgagca aatcacccgc ccgttatcag
     3721 aatacaaagc aattggcccg catgtagctg tggccaaacg cctggccgcg aaaggcgtaa
     3781 aagttaaacc gggtatggta attgggtaca tcgtcctgaa gggagatggt ccaatcagca
     3841 aacgtgccat tgctattgaa gagtttgatc cgaaaaaaca caaatacgac gccgaatact
     3901 atattgaaaa ccaagttctg ccggccgttg aacgtatcct gcgcgcgttc ggctaccgta
     3961 aggaggatct gcgctaccaa aaaaccaaac aggtgggtct gggtgcttgg ctgaaattcc
     4021 accaccacca ccaccactaa ccgctgagca ataactagca taaccccttg gggcctctaa
     4081 acgggtcttg aggggttttt tgccccagga tacatagatt accacaactc cgagcccttc
     4141 caccactagt cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc
     4201 ttggggcctc taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag
     4261 cttggcaggt gatgacacac attaacaaat ttcgtgagga gtctccagaa gaatgccatt
     4321 aatttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4381 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4441 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4501 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4561 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4621 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4681 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4741 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4801 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4861 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaaggccta
     4921 ctattagcaa caacgatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4981 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5041 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5101 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5161 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5221 ccagtgctgc aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa
     5281 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5341 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5401 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5461 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5521 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5581 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5641 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5701 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5761 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5821 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5881 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5941 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6001 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6061 ttccgcgcac ctgcaccagt cagtaaaacg acggccagta gtcaaaagcc tccgaccgga
     6121 ggcttttgac ttggttcagg tggagtggga g
//
