LOCUS       Exported     6302 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..6302
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      4339..4386
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     6234..6250
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      6251..6282
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     promoter        1454..1482
                     /label="tac promoter"
                     /note="tac promoter"
     CDS             1576..4143
                     /label="RNAseInhib"
                     /note="RNAse Inhibitor (murine sequence, need Rosetta E. coli strain to be expressed). Protects RNA from degradation"
     primer_bind     complement(4391..4407)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(4474..5062)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(5253..6113)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(6114..6218)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 TTAATTAACA TTACTCGCAT CCATTCTCAG GCTGTCTCGT CTCGTCTCCC GACACCATCG
       61 AATGGTGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC GGAAGAGAGT CAATTCAGGG
      121 TGGTGAATGT GAAACCAGTA ACGTTATACG ATGTCGCAGA GTATGCCGGT GTCTCTTATC
      181 AGACCGTTTC CCGCGTGGTG AACCAGGCCA GCCACGTTTC TGCGAAAACG CGGGAAAAAG
      241 TGGAAGCGGC GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA CAACTGGCGG
      301 GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC
      361 AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG TGCCAGCGTG GTGGTGTCGA
      421 TGGTAGAACG AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT GCACAATCTT CTCGCGCAAC
      481 GCGTCAGTGG GCTGATCATT AACTATCCGC TGGATGACCA GGATGCCATT GCTGTGGAAG
      541 CTGCCTGCAC TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA CCCATCAACA
      601 GTATTATTTT CTCCCATGAA GATGGTACGC GACTGGGCGT GGAGCATCTG GTCGCATTGG
      661 GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC TGTCTCGGCG CGTCTGCGTC
      721 TGGCTGGCTG GCATAAATAT CTCACTCGCA ATCAAATTCA GCCGATAGCG GAACGGGAAG
      781 GCGACTGGAG TGCCATGTCC GGTTTTCAAC AAACCATGCA AATGCTGAAT GAGGGCATCG
      841 TTCCCACTGC GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG CGCGCCATTA
      901 CCGAGTCCGG GCTGCGCGTT GGTGCGGATA TCTCGGTAGT GGGATACGAC GATACCGAAG
      961 ATAGCTCATG TTATATCCCG CCGTTAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC
     1021 AAACCAGCGT GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC
     1081 TGTTGCCCGT GTCACTGGTG AAAAGAAAAA CCACCCTGGC GCCCAATACG CAAACCGCCT
     1141 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA
     1201 GCGGGCAGTG AGCGCAACGC AATTAATGTA AGTTAGCTCA CTCATTAGGC ACAATTCTCA
     1261 TGTTTGACAG CTTATCATCG ACTGCACGGT GCACCAATGC TTCTGGCGTC AGGCAGCCAT
     1321 CGGAAGCTGT GGTATGGCTG TGCAGGTCGT AAATCACTGC ATAATTCGTG TCGCTCAAGG
     1381 CGCACTCCCG TTCTGGATAA TGTTTTTTGC GCCGACATCA TAACGGTTCT GGCAAATATT
     1441 CTGAAATGAG CTGTTGACAA TTAATCATCG GCTCGTATAA TGTGTGGAAT TGTGAGCGGA
     1501 TAACAATTTC ACACAGGAAA CAGCCAGTCC GTTTAGGTGT TTTCACGAGC AATTGACCAA
     1561 CAAGGACCAT AGATTATGCA CCACCACCAC CACCACAAAA TCGAAGAAGG TAAACTGGTA
     1621 ATCTGGATTA ACGGCGATAA AGGCTATAAC GGCCTCGCGG AAGTCGGTAA GAAATTCGAG
     1681 AAAGATACCG GAATTAAAGT CACCGTTGAG CATCCGGATA AACTGGAAGA GAAATTCCCA
     1741 CAGGTTGCGG CAACTGGCGA TGGCCCTGAC ATTATCTTCT GGGCACACGA CCGCTTTGGT
     1801 GGCTACGCTC AATCTGGCCT GTTGGCTGAA ATCACCCCGG ACAAAGCGTT CCAGGACAAG
     1861 CTGTATCCGT TTACCTGGGA TGCCGTACGT TACAACGGCA AGCTGATTGC TTACCCGATC
     1921 GCTGTTGAAG CGTTATCGCT GATTTATAAC AAAGACCTGC TGCCGAACCC GCCAAAAACC
     1981 TGGGAAGAGA TCCCGGCGCT GGATAAAGAA CTGAAAGCGA AAGGTAAGAG CGCGCTGATG
     2041 TTCAACCTGC AAGAACCGTA CTTCACCTGG CCGCTGATTG CTGCTGACGG GGGTTATGCG
     2101 TTCAAGTATG AAAACGGCAA GTACGACATT AAAGACGTGG GCGTGGATAA CGCTGGCGCG
     2161 AAAGCGGGTC TGACCTTCCT GGTTGACCTG ATTAAAAACA AACACATGAA TGCAGACACC
     2221 GATTACTCCA TCGCAGAAGC TGCCTTTAAT AAAGGCGAAA CAGCGATGAC CATCAACGGC
     2281 CCGTGGGCAT GGTCCAACAT CGACACCAGC AAAGTGAATT ATGGTGTAAC GGTACTGCCG
     2341 ACCTTCAAGG GTCAACCATC CAAACCGTTC GTTGGCGTGC TGAGCGCAGG TATTAACGCC
     2401 GCCAGTCCGA ACAAAGAGCT GGCAAAAGAG TTCCTCGAAA ACTATCTGCT GACTGATGAA
     2461 GGTCTGGAAG CGGTTAATAA AGACAAACCG CTGGGTGCCG TAGCGCTGAA GTCTTACGAG
     2521 GAAGAGTTGG TGAAAGATCC GCGTATTGCC GCCACTATGG AAAACGCCCA GAAAGGTGAA
     2581 ATCATGCCGA ACATCCCGCA GATGTCCGCT TTCTGGTATG CCGTGCGTAC TGCGGTGATC
     2641 AACGCCGCCA GCGGTCGTCA GACTGTCGAT GAAGCCCTGA AAGACGCGCA GACTAATTCG
     2701 AGCTCGAACA ACAACAACAA TAACAATAAC AACAACCTCG GGATTGAGGG AAGGGAAAAT
     2761 CTTTATTTTC AAGGTAGTCT TGACATCCAG TGTGAGCAGC TGAGTGATGC CCGGTGGACA
     2821 GAGCTCCTTC CTCTGATCCA GCAATACGAA GTGGTCAGGC TGGATGACTG TGGCCTCACT
     2881 GAAGTGCGGT GCAAAGACAT CAGCTCAGCA GTCCAAGCCA ACCCTGCCCT GACAGAGCTC
     2941 AGCCTTCGCA CCAATGAACT GGGTGATGGC GGTGTGGGTC TGGTGCTCCA GGGCCTGCAA
     3001 AATCCCACTT GTAAGATCCA GAAGCTGAGC CTTCAGAACT GCGGCTTGAC GGAGGCTGGC
     3061 TGTGGGATTC TGCCTGGCAT GCTGCGCTCT TTGTCTACCC TGCGTGAGCT ACATCTCAAT
     3121 GATAACCCTA TGGGGGACGC AGGCCTGAAG CTGCTCTGTG AAGGACTTCA GGACCCCCAG
     3181 TGCCGTCTTG AGAAGCTTCA GTTGGAATAC TGTAACCTCA CAGCTACCAG CTGCGAGCCC
     3241 CTGGCTTCAG TGCTCAGGGT GAAAGCTGAC TTTAAGGAGC TAGTATTGAG CAACAACGAC
     3301 CTCCATGAAC CTGGTGTCCG TATTCTGTGC CAGGGCCTGA AGGACTCTGC CTGTCAACTG
     3361 GAGTCACTCA AGCTGGAGAA CTGTGGTATC ACAGCAGCCA ACTGCAAGGA TCTGTGCGAT
     3421 GTTGTGGCCT CCAAAGCTTC GCTACAGGAA CTGGACTTGA GCAGCAACAA GCTAGGTAAT
     3481 GCAGGCATTG CAGCACTGTG CCCAGGACTG CTGCTTCCCA GCTGTAAGCT CAGGACTCTG
     3541 TGGCTCTGGG AATGTGATAT CACTGCGGAG GGCTGCAAGG ACCTGTGCCG TGTCCTCAGA
     3601 GCCAAGCAGA GCCTGAAGGA ACTCAGCCTT GCTTCCAATG AGCTGAAGGA TGAGGGTGCC
     3661 CGGCTGCTGT GTGAGAGCCT ATTAGAGCCT GGCTGTCAGC TGGAGTCACT GTGGATAAAG
     3721 ACCTGTAGCC TCACAGCTGC CTCCTGTCCC TACTTCTGCT CCGTGTTGAC CAAAAGCCGT
     3781 TCTCTGTTGG AGCTGCAAAT GAGCAGCAAC CCACTGGGAG ATGAGGGAGT CCAGGAGCTT
     3841 TGCAAGGCCC TGAGCCAGCC AGATACAGTG CTGCGTGAGC TTTGGTTGGG AGACTGTGAT
     3901 GTGACAAATA GTGGTTGCAG CAGCCTTGCC AATGTCCTGC TGGCCAACCG CAGCCTGAGG
     3961 GAACTGGACC TCAGTAACAA CTGCATGGGG GGCCCAGGTG TCCTGCAACT GCTGGAGAGC
     4021 CTCAAACAGC CCAGCTGCAC CCTTCAGCAG CTCGTCCTGT ATGACATTTA CTGGACAAAT
     4081 GAGGTGGAAG AACAGCTTCG GGCCCTGGAG GAGGAAAGGC CTTCCCTGAG GATCATTTCC
     4141 TAACCTGCAG GTAATTAAAT AAGCTTCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG
     4201 CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCCCAGG
     4261 ATACATAGAT TACCACAACT CCGAGCCCTT CCACCACTAG TCGCTGAGGT GTCAATCGTC
     4321 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     4381 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     4441 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     4501 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     4561 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     4621 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     4681 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     4741 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     4801 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     4861 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     4921 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     4981 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     5041 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     5101 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     5161 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     5221 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     5281 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     5341 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     5401 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     5461 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     5521 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     5581 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     5641 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     5701 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     5761 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     5821 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     5881 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     5941 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     6001 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     6061 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     6121 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     6181 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     6241 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG
     6301 AG
//