LOCUS Exported 2665 bp ds-DNA circular SYN 24-SEP-2020 DEFINITION synthetic linear DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2665) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. REFERENCE 2 (bases 1 to 2665) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Sep 24, 2020 from SnapGene 5.0.7 https://www.snapgene.com FEATURES Location/Qualifiers source 1..2665 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..2665 /label=T7 terminator /note="""transcription terminator for bacteriophage T7 RNA" promoter 49..67 /label=T7 promoter /note="t7 promoter" misc_feature 68..602 /label=T7_Orf1A_1 terminator 603..650 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(754..770) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" rep_origin complement(837..1425) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1616..2476) /codon_start=1 /label=bla /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2477..2581) /label=bla promoter /note="ampicillin resistance gene promoter" primer_bind 2597..2613 /label=M13 for /note="common sequencing primer, one of multiple similar variants" terminator 2614..2645 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" ORIGIN 1 tcgtcactag agggccctgc attaggatcg atctcgatcc cgcgaaatta atacgactca 61 ctatagggga aaggtaagat ggagagcctt gtccctggtt tcaacgagaa aacacacgtc 121 caactcagtt tgcctgtttt acaggttcgc gacgtgctcg tacgtggctt tggagactcc 181 gtggaggagg tcttatcaga ggcacgtcaa catcttaaag atggcacttg tggcttagta 241 gaagttgaaa aaggcgtttt gcctcaactt gaacagccct atgtgttcat caaacgttcg 301 gatgctcgaa ctgcacctca tggtcatgtt atggttgagc tggtagcaga actcgaaggc 361 attcagtacg gtcgtagtgg tgagacactt ggtgtccttg tccctcatgt gggcgaaata 421 ccagtggctt accgcaaggt tcttcttcgt aagaacggta ataaaggagc tggtggccat 481 agttacggcg ccgatctaaa gtcatttgac ttaggcgacg agcttggcac tgatccttat 541 gaagattttc aagaaaactg gaacactaaa catagcgctt cgcctaggcc gctgagcaat 601 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaacct 661 cgctcgctga ggtgtcaatc gtcggagccg ctgagcaata actagcataa ccccttgggg 721 cctctaaacg ggtcttgagg ggttttttgc atggtcatag ctgtttcctg agagcttggc 781 aggtgatgac acacattaac aaatttcgtg aggagtctcc agaagaatgc cattaatttc 841 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 901 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 961 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 1021 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 1081 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 1141 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 1201 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 1261 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 1321 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 1381 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaagg cctactatta 1441 gcaacaacga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 1501 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 1561 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 1621 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 1681 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 1741 ctgcaatgat accgcgagaa ccacgctcac cggctccaga tttatcagca ataaaccagc 1801 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 1861 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 1921 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 1981 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 2041 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 2101 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 2161 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 2221 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 2281 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 2341 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 2401 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 2461 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 2521 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 2581 gcacctgcac cagtcagtaa aacgacggcc agtagtcaaa agcctccgac cggaggcttt 2641 tgacttggtt caggtggagt gggag //