LOCUS       Exported     2704 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2704
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      642..689
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2636..2652
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2653..2684
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     promoter        49..67
                     /label="T7 promoter"
                     /note="t7 promoter"
     CDS             100..2703
                     /label="T7_Orf1A_2"
                     /note="PolT7-driven expression  of a 269nt part of the Sars-CoV-2 OrfA RNA, to be used as control"
     primer_bind     complement(793..809)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(876..1464)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1655..2515)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2516..2620)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 TCGTCACTAG AGGGCCCTGC ATTAGGATCG ATCTCGATCC CGCGAAATTA ATACGACTCA
       61 CTATAGGGGA TCAAGAATCC TTTGGTGGTG CATCGTGTTG TCTGTACTGC CGTTGCCACA
      121 TAGATCATCC AAATCCTAAA GGATTTTGTG ACTTAAAAGG TAAGTATGTA CAAATACCTA
      181 CAACTTGTGC TAATGACCCT GTGGGTTTTA CACTTAAAAA CACAGTCTGT ACCGTCTGCG
      241 GTATGTGGAA AGGTTATGGC TGTAGTTGTG ATCAACTCCG CGAACCCATG CTTCAGTCAG
      301 CTGATGCACA ATCGTTTTTA AACGGGTTTG CGGTGTAAGT GCAGCCCGTC TTACACCGTG
      361 CGGCACAGGC ACTAGTACTG ATGTCGTATA CAGGGCTTTT GACATCTACA ATGATAAAGT
      421 AGCTGGTTTT GCTAAATTCC TAAAAACTAA TTGTTGTCGC TTCCAAGAAA AGGACGAAGA
      481 TGACAATTTA ATTGATTCTT ACTTTGTAGT TAAGAGACAC ACTTTCTCTA ACTACCAACA
      541 TGAAGAAACA ATTTATAATT TACTTAAGGA TTGTCCAGCT GTTGCTAAAC ATGACTTCTT
      601 TAAGTTTAGA ATAGAGCTTC GCCTAGGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG
      661 CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAACCTC GCTCGCTGAG GTGTCAATCG
      721 TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG
      781 GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA
      841 AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC
      901 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
      961 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
     1021 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC
     1081 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
     1141 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
     1201 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
     1261 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA
     1321 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT
     1381 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
     1441 ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT
     1501 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
     1561 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
     1621 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
     1681 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
     1741 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC
     1801 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
     1861 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
     1921 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG
     1981 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
     2041 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG
     2101 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
     2161 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT
     2221 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA
     2281 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
     2341 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
     2401 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
     2461 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
     2521 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
     2581 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA
     2641 ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTTC AGGTGGAGTG
     2701 GGAG
//