LOCUS Exported 2514 bp ds-DNA circular SYN 24-SEP-2020 DEFINITION synthetic linear DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2514) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. REFERENCE 2 (bases 1 to 2514) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Sep 24, 2020 from SnapGene 5.0.7 https://www.snapgene.com FEATURES Location/Qualifiers source 1..2514 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..2514 /label=T7 terminator /note="""transcription terminator for bacteriophage T7 RNA" promoter 49..67 /label=T7 promoter /note="t7 promoter" misc_feature 68..451 /label=T7_Orf1B_3 terminator 452..499 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(603..619) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" rep_origin complement(686..1274) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1465..2325) /codon_start=1 /label=bla /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2326..2430) /label=bla promoter /note="ampicillin resistance gene promoter" primer_bind 2446..2462 /label=M13 for /note="common sequencing primer, one of multiple similar variants" terminator 2463..2494 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" ORIGIN 1 tcgtcactag agggccctgc attaggatcg atctcgatcc cgcgaaatta atacgactca 61 ctatagggtc aaaaattatt gaaatcaata gccgccacta gaggagctac tgtagtaatt 121 ggaacaagca aattctatgg tggttggcac aacatgttaa aaactgttta tagtgatgta 181 gaaaaccctc accttatggg ttgggattat cctaaatgtg atagagccat gcctaacatg 241 cttagaatta tggcctcact tgttcttgct cgcacacata caacgtgttg tagcttgtca 301 caccgtttct atagattagc taatgagtgt gctcaagtat tgagtgaaat ggtcatgtgt 361 ggcggttcac tatatgttaa accaggtgga acctcatcag gagatgccac aactgcttat 421 gctaagcttc gcctaggccg ctgagcaata actagcataa ccccttgggg cctctaaacg 481 ggtcttgagg ggttttttgc tgaaaacctc gctcgctgag gtgtcaatcg tcggagccgc 541 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgca 601 tggtcatagc tgtttcctga gagcttggca ggtgatgaca cacattaaca aatttcgtga 661 ggagtctcca gaagaatgcc attaatttcc ataggctccg cccccctgac gagcatcaca 721 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 781 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 841 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 901 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 961 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 1021 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 1081 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 1141 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 1201 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 1261 aaaaaggatc tcaagaaggc ctactattag caacaacgat cctttgatct tttctacggg 1321 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 1381 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 1441 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 1501 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 1561 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagaac cacgctcacc 1621 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 1681 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 1741 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 1801 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 1861 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 1921 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 1981 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 2041 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 2101 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 2161 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 2221 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 2281 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 2341 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 2401 ttagaaaaat aaacaaatag gggttccgcg cacctgcacc agtcagtaaa acgacggcca 2461 gtagtcaaaa gcctccgacc ggaggctttt gacttggttc aggtggagtg ggag //