LOCUS       Exported                2455 bp ds-DNA     circular SYN 24-SEP-2020
DEFINITION  synthetic linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 24, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..2455
                     /label=T7 terminator
                     /note="""transcription terminator for bacteriophage T7 RNA"
     promoter        49..67
                     /label=T7 promoter
                     /note="t7 promoter"
     misc_feature    68..392
                     /label=T7_Orf1E_4
     terminator      393..440
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(544..560)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(627..1215)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1406..2266)
                     /codon_start=1
                     /label=bla
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2267..2371)
                     /label=bla promoter
                     /note="ampicillin resistance gene promoter"
     primer_bind     2387..2403
                     /label=M13 for
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2404..2435
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 tcgtcactag agggccctgc attaggatcg atctcgatcc cgcgaaatta atacgactca
       61 ctatagggca gtaatggaac caatttatga tgaaccgacg acgactacta gcgtgccttt
      121 gtaagcacaa gctgatgagt acgaacttat gtactcattc gtttcggaag agacaggtac
      181 gttaatagtt aatagcgtac ttctttttct tgctttcgtg gtattcttgc tagttacact
      241 agccatcctt actgcgcttc gattgtgtgc gtactgctgc aatattgtta acgtgagtct
      301 tgtaaaacct tctttttacg tttactctcg tgttaaaaat ctgaattctt ctagagttcc
      361 tgatctgctt cgcctaggcc gctgagcaat aactagcata accccttggg gcctctaaac
      421 gggtcttgag gggttttttg ctgaaaacct cgctcgctga ggtgtcaatc gtcggagccg
      481 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
      541 atggtcatag ctgtttcctg agagcttggc aggtgatgac acacattaac aaatttcgtg
      601 aggagtctcc agaagaatgc cattaatttc cataggctcc gcccccctga cgagcatcac
      661 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      721 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      781 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      841 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      901 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      961 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1021 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1081 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1141 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1201 aaaaaaggat ctcaagaagg cctactatta gcaacaacga tcctttgatc ttttctacgg
     1261 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1321 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1381 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1441 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1501 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagaa ccacgctcac
     1561 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     1621 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     1681 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     1741 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     1801 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     1861 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     1921 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     1981 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     2041 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     2101 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     2161 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     2221 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     2281 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     2341 tttagaaaaa taaacaaata ggggttccgc gcacctgcac cagtcagtaa aacgacggcc
     2401 agtagtcaaa agcctccgac cggaggcttt tgacttggtt caggtggagt gggag
//
