LOCUS Exported 3257 bp ds-DNA circular SYN 24-SEP-2020 DEFINITION synthetic linear DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3257) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. REFERENCE 2 (bases 1 to 3257) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Sep 24, 2020 from SnapGene 5.0.7 https://www.snapgene.com FEATURES Location/Qualifiers source 1..3257 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..3257 /label=T7 terminator /note="""transcription terminator for bacteriophage T7 RNA" promoter 49..67 /label=T7 promoter /note="t7 promoter" misc_feature 68..1194 /label=T7_OrfN_5 terminator 1195..1242 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(1346..1362) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1429..2017) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2208..3068) /codon_start=1 /label=bla /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3069..3173) /label=bla promoter /note="ampicillin resistance gene promoter" primer_bind 3189..3205 /label=M13 for /note="common sequencing primer, one of multiple similar variants" terminator 3206..3237 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" ORIGIN 1 tcgtcactag agggccctgc attaggatcg atctcgatcc cgcgaaatta atacgactca 61 ctatagggat gtctgataat ggaccccaaa atcagcgaaa tgcaccccgc attacgtttg 121 gtggaccctc agattcaact ggcagtaacc agaatggaga acgcagtggg gcgcgatcaa 181 aacaacgtcg gccccaaggt ttacccaata atactgcgtc ttggttcacc gctctcactc 241 aacatggcaa ggaagacctt aaattccctc gaggacaagg cgttccaatt aacaccaata 301 gcagtccaga tgaccaaatt ggctactacc gaagagctac cagacgaatt cgtggtggtg 361 acggtaaaat gaaagatctc agtccaagat ggtatttcta ctacctagga actgggccag 421 aagctggact tccctatggt gctaacaaag acggcatcat atgggttgca actgagggag 481 ccttgaatac accaaaagat cacattggca cccgcaatcc tgctaacaat gctgcaatcg 541 tgctacaact tcctcaagga acaacattgc caaaaggctt ctacgcagaa gggagcagag 601 gcggcagtca agcctcttct cgttcctcat cacgtagtcg caacagttca agaaattcaa 661 ctccaggcag cagtagggga acttctcctg ctagaatggc tggcaatggc ggtgatgctg 721 ctcttgcttt gctgctgctt gacagattga accagcttga gagcaaaatg tctggtaaag 781 gccaacaaca acaaggccaa actgtcacta agaaatctgc tgctgaggct tctaagaagc 841 ctcggcaaaa acgtactgcc actaaagcat acaatgtaac acaagctttc ggcagacgtg 901 gtccagaaca aacccaagga aattttgggg accaggaact aatcagacaa ggaactgatt 961 acaaacattg gccgcaaatt gcacaatttg cccccagcgc ttcagcgttc ttcggaatgt 1021 cgcgcattgg catggaagtc acaccttcgg gaacgtggtt gacctacaca ggtgccatca 1081 aattggatga caaagatcca aatttcaaag atcaagtcat tttgctgaat aagcatattg 1141 acgcatacaa aacattccca ccaacagagc ttcgcctagg ccgctgagca ataactagca 1201 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaaac ctcgctcgct 1261 gaggtgtcaa tcgtcggagc cgctgagcaa taactagcat aaccccttgg ggcctctaaa 1321 cgggtcttga ggggtttttt gcatggtcat agctgtttcc tgagagcttg gcaggtgatg 1381 acacacatta acaaatttcg tgaggagtct ccagaagaat gccattaatt tccataggct 1441 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 1501 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 1561 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 1621 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 1681 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 1741 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 1801 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 1861 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 1921 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 1981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa ggcctactat tagcaacaac 2041 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 2101 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 2161 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 2221 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 2281 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 2341 ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca gccagccgga 2401 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 2461 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 2521 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 2581 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 2641 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 2701 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 2761 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 2821 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 2881 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 2941 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 3001 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 3061 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 3121 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacctgc 3181 accagtcagt aaaacgacgg ccagtagtca aaagcctccg accggaggct tttgacttgg 3241 ttcaggtgga gtgggag //