LOCUS       Exported                3442 bp ds-DNA     circular SYN 24-SEP-2020
DEFINITION  synthetic linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 24, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..3442
                     /label=T7 terminator
                     /note="""transcription terminator for bacteriophage T7 RNA"
     promoter        49..67
                     /label=T7 promoter
                     /note="t7 promoter"
     CDS             93..1352
                     /codon_start=1
                     /label=Sars_NegativeControl
                     /translation="MSDNGPQNQRSAPRITFGGPSDSSDNSKNGERNGARPKQRRPQGL
                     PNNTASWFTALTQHGKENLTFPRGQGVPINTNSSKDDQIGYYRRATRRIRGGDGKMKEL
                     SPRWYFYYLGTGPEAGLPYGANKEGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQ
                     GTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDTALALL
                     LLDRLNQLENKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQ
                     TQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLD
                     DKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKADELQALPQRQKKQQTVTLLPAAD
                     LDEFSKQLQQSMSGTDSTQA"
     terminator      1380..1427
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1531..1547)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1614..2202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2393..3253)
                     /codon_start=1
                     /label=bla
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3254..3358)
                     /label=bla promoter
                     /note="ampicillin resistance gene promoter"
     primer_bind     3374..3390
                     /label=M13 for
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      3391..3422
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 tcgtcactag agggccctgc attaggatcg atctcgatcc cgcgaaatta atacgactca
       61 ctatagggtt gcgtttgaga cgggcgacag atatgtctga taatggaccc caaaaccaac
      121 gtagtgcacc ccgcattaca tttggtggac cctcagattc aagtgacaat agcaaaaacg
      181 gagagcgcaa tggtgcacga cctaaacaac gtcgacccca aggcttaccc aataatactg
      241 catcttggtt caccgctctc actcaacatg gcaaggaaaa ccttacgttc cctcgagggc
      301 aaggtgttcc aatcaacacc aatagctcta aagatgacca aattggctac taccgtagag
      361 ctaccagacg aattcgtggt ggtgacggta aaatgaaaga gctcagcccc agatggtatt
      421 tttactatct aggaactgga ccagaagctg gacttcccta tggtgctaac aaagaaggca
      481 tcatatgggt tgcaactgag ggagccttaa acacaccgaa agaccacatt ggcacccgca
      541 atcctgctaa caatgctgca atcgtgctac aacttcctca aggaacaaca ttgccaaaag
      601 gcttctacgc agaagggagc agaggcggca gtcaagcttc ttcacgctcc tcatcacgta
      661 gtcgcaacag ttcaagaaac tcaactccag gcagcagtag gggaacttct cctgctagaa
      721 tggctggcaa tggcggtgac actgctcttg ctttgctgct gctagatagg ttgaaccagc
      781 ttgagaacaa agtatctggc aaaggccaac aacaacaggg ccaaactgtc actaagaaat
      841 ctgctgctga ggcatctaaa aagcctcgcc aaaaacgtac tgctacaaaa cagtacaacg
      901 tcactcaagc atttgggaga cgtggtccag aacaaaccca aggaaatttt ggggaccaag
      961 aattaatcag acaaggaact gattacaaac attggccgca aattgcacaa tttgctccaa
     1021 gtgcctctgc attctttgga atgtcacgca ttggcatgga agtcacacct tcgggaacat
     1081 ggctgactta tcatggagcc attaaattgg atgacaaaga tccacaattc aaagataacg
     1141 tcatactgct gaataagcac attgacgcat acaaaacatt cccaccaaca gagcctaaaa
     1201 aggacaaaaa gaaaaaggct gatgaacttc aggctttacc gcagagacag aagaaacaac
     1261 aaactgtgac ccttcttcct gctgcagatt tggatgaatt ctccaaacag ttgcaacaat
     1321 ccatgagtgg tactgattca acccaggctt aaagcttcgc ctaggccgct gagcaataac
     1381 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaacctcgc
     1441 tcgctgaggt gtcaatcgtc ggagccgctg agcaataact agcataaccc cttggggcct
     1501 ctaaacgggt cttgaggggt tttttgcatg gtcatagctg tttcctgaga gcttggcagg
     1561 tgatgacaca cattaacaaa tttcgtgagg agtctccaga agaatgccat taatttccat
     1621 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1681 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1741 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1801 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1861 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1921 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1981 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2041 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2101 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2161 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaaggcct actattagca
     2221 acaacgatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2281 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2341 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2401 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2461 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2521 caatgatacc gcgagaacca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2581 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2641 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2701 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2761 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2821 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2881 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2941 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3001 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3061 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3121 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3181 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3241 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3301 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3361 cctgcaccag tcagtaaaac gacggccagt agtcaaaagc ctccgaccgg aggcttttga
     3421 cttggttcag gtggagtggg ag
//
