LOCUS Exported 10683 bp ds-DNA circular SYN 23-FEB-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10683) AUTHORS David Gordon TITLE Direct Submission JOURNAL Exported Wednesday, Mar 25, 2020 from SnapGene Viewer 5.0.8 https://www.snapgene.com FEATURES Location/Qualifiers source 1..10683 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..9 /label=Kozak CDS 10..3828 /codon_start=1 /label=nCoV-2019-S /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRS SVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGW IFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSA NNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSA LEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNE NGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGE VFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADS FVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRK SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFEL LHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAV RDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRV YSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIA YTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLL LQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKP SKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIA QYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSA IGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEA EVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYH LMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQR NFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDL GDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLI AIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT" CDS 3847..3870 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" CDS 3907..3930 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" polyA_signal 3999..4120 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" terminator 4235..4281 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 4311..4447 /label=rrnG terminator /note="transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)" misc_feature 4492..4563 /label=3' beta-globin insulator /note="insulator downstream of the human beta-globin locus (Farrell et al., 2002)" rep_origin complement(4789..5377) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 5877..7086 /label=UbC promoter /note="human ubiquitin C promoter" CDS 7090..7689 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" polyA_signal 7716..7764 /note="synthetic polyadenylation signal" CDS 8030..8890 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" terminator 8903..9039 /label=rrnG terminator /note="transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)" misc_feature 9184..9255 /label=5' beta-globin insulator /note="insulator upstream of the human beta-globin locus (Farrell et al., 2002)" terminator 9260..9306 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" polyA_signal 9324..9372 /note="synthetic polyadenylation signal" promoter 9409..10587 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 9640..10578 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" ORIGIN 1 gccgccacca tgtttgtttt cttggttctt ttgccactgg taagcagtca gtgcgtcaac 61 ctgaccaccc ggacacaact cccacccgca tacacgaaca gtttcactag gggggtttac 121 taccctgaca aagtttttag atctagtgtt ttgcattcca cgcaagatct gttccttccg 181 ttcttttcaa atgtcacgtg gttccatgct attcacgtca gcggcactaa tggaacgaaa 241 cgattcgata accccgtctt gcccttcaac gatggcgtat atttcgcgtc aacagagaaa 301 agtaatataa ttagaggctg gatctttggt acgactctcg acagtaagac gcagtcactt 361 ctgattgtaa acaatgcaac taatgtagtc atcaaggtgt gtgagttcca gttctgcaat 421 gatcctttct tgggagtgta ttatcataag aataacaaat cctggatgga gtctgagttc 481 cgggtatatt cttcagcaaa caattgcacg tttgaatatg taagccaacc tttccttatg 541 gacctggaag ggaagcaagg taatttcaag aatcttaggg aatttgtgtt taaaaatata 601 gatggctact tcaagatcta ctcaaagcat accccgatta acctcgtcag agacctgccc 661 caagggttca gtgcgcttga acccctggta gatttgccaa tcggcataaa tataaccagg 721 ttccagacac tgctcgccct ccaccggagc taccttactc caggagattc atctagcggg 781 tggacagctg gagcggcagc ttactacgtc ggttaccttc aaccgaggac attcttgctt 841 aaatacaatg agaatggcac gataactgac gccgtagatt gcgcgcttga tccgctgtca 901 gaaacgaagt gtacacttaa aagttttacg gtcgaaaagg gaatctacca aacatcaaat 961 tttagggtac aacctacgga atctatcgta cgcttcccga atatcactaa cttgtgtccg 1021 ttcggcgagg tttttaatgc gaccaggttt gcttccgtgt acgcctggaa caggaaacgg 1081 atctccaatt gtgtcgccga ttactccgtc ttgtataatt cagcatcttt cagcacgttt 1141 aaatgttacg gagtttcccc cacaaaattg aatgaccttt gctttacgaa cgtctacgcg 1201 gattcatttg taatccgggg ggacgaagtt aggcaaattg cgccagggca gactggcaag 1261 atagctgact ataattataa attgccggat gactttacgg gctgtgtgat tgcttggaac 1321 tcaaataatc tggactcaaa ggtaggggga aattataact acctttacag gctgttccgg 1381 aagagtaatc tgaagccatt cgaaagagat ataagtacag agatctacca agctggaagc 1441 accccctgca atggtgttga aggattcaat tgttatttcc cattgcaatc ctatggtttt 1501 caaccgacga atggggtggg ataccaacca tatcgagttg tggttctcag tttcgagttg 1561 cttcatgctc ctgcgacagt atgtggacca aaaaaatcta ctaatctggt gaagaataaa 1621 tgcgtcaatt ttaattttaa tggtctgaca ggtaccggag tgcttacgga atctaacaag 1681 aagtttcttc cttttcaaca gttcgggagg gacatagccg acacaactga cgcggtaaga 1741 gatccgcaga cgttggaaat tctcgatata actccatgct catttggagg agtgagtgtt 1801 atcactcctg gtacgaacac cagtaatcag gttgcagttc tttatcagga cgtgaattgt 1861 acagaggtcc cggttgcgat acacgcggac cagctgacgc ccacgtggcg ggtttactca 1921 actgggtcaa atgtgtttca aaccagagcc ggctgcctca taggagccga gcatgtgaac 1981 aacagttatg aatgtgacat acctatcggg gcggggatct gcgcttcata tcaaacgcaa 2041 accaattccc cgcgaagagc gcgaagtgtt gcaagtcaaa gtattatagc gtatacgatg 2101 agcttgggtg ctgagaactc cgtggcatat tcaaataatt ccatcgcaat tcccacgaat 2161 tttacgattt ctgtgacgac ggagatattg ccggtttcaa tgactaagac gagtgttgac 2221 tgtacgatgt acatctgcgg cgactcaacg gagtgttcca acctgctcct gcagtacggc 2281 tcattctgca cccaactgaa tcgggcactt acgggtattg ctgttgaaca agacaaaaat 2341 acgcaagagg tgttcgccca ggtcaagcag atctacaaga caccaccgat taaggacttt 2401 ggggggttta atttttctca gatacttccc gatcccagta aacctagtaa aaggtcattt 2461 atagaggacc tcttgttcaa caaggtaacg ctcgctgatg caggattcat caagcagtat 2521 ggcgattgtc ttggggacat tgcggcgcgc gacctgattt gtgcccagaa gttcaacgga 2581 ctgacggtac ttccgcctct cctgaccgac gaaatgatcg cacaatacac ttctgcattg 2641 cttgctggca ccataacctc aggttggacc ttcggtgccg gggcggcctt gcaaatcccc 2701 tttgctatgc aaatggcata tcgattcaat gggataggag ttactcaaaa tgttctgtat 2761 gaaaaccaaa agctgatagc caatcaattt aatagtgcta ttgggaaaat tcaggacagc 2821 ctgagtagta cggcttccgc tctcggcaaa ctccaagatg tcgttaacca aaatgcacaa 2881 gctctcaata cacttgttaa gcaactgagc tccaacttcg gcgctatatc atcagttctt 2941 aacgatattc tttcccgact cgacaaggtc gaagccgaag tccaaataga caggctgata 3001 accgggaggc tgcaaagtct ccaaacgtat gttacccaac aactcatacg agcggccgag 3061 attagggcgt cagccaacct cgccgctact aaaatgtctg aatgtgtact cgggcagtcc 3121 aagcgcgtgg acttttgtgg caagggttat catctgatga gtttcccgca atcagcgcct 3181 cacggagtgg tcttcctcca cgtgacgtat gtccctgcgc aagaaaaaaa ctttacgaca 3241 gcccctgcca tttgtcatga cgggaaggcg cactttccac gcgaaggcgt ttttgtatcc 3301 aacggcaccc actggtttgt cacacagcga aatttttatg agcctcagat cataactact 3361 gacaatactt tcgtctcagg gaactgcgat gtagtcatag ggatcgtaaa caatacggta 3421 tacgatcccc tccagcctga actggactca tttaaagagg agctggacaa gtatttcaaa 3481 aatcacacat caccggacgt ggacctcgga gatattagtg gtattaacgc aagcgtagta 3541 aatattcaaa aggaaatcga ccgcttgaat gaagtcgcga aaaacctgaa cgagtctctt 3601 attgatcttc aagaactcgg taagtatgaa caatacatca agtggccttg gtacatatgg 3661 cttggattca ttgcggggtt gatcgcaatc gtaatggtta ccattatgtt gtgttgcatg 3721 acaagctgtt gcagctgcct caaaggttgt tgttcttgcg gtagttgctg caagttcgac 3781 gaagatgatt ctgaacctgt tcttaaaggc gtaaaattgc actacacgct cgaaggcggc 3841 gggggatgga gccatccaca attcgagaaa ggcggtggtt caggaggagg tagcgggggt 3901 ggatcatggt cacatccgca gtttgaaaag taaggatccg caggcctctg ctagcttgac 3961 tgactgagat acagcgtacc ttcagctcac agacatgata agatacattg atgagtttgg 4021 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 4081 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 4141 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta 4201 caaatgtggt attggcccat ctctatcggt atcgtagcat aaccccttgg ggcctctaaa 4261 cgggtcttga ggggtttttt gtgcccctcg ggccggattg ctatctaccg gcattggcgc 4321 agaaaaaaat gcctgatgcg acgctgcgcg tcttatactc ccacatatgc cagattcagc 4381 aacggatacg gcttccccaa cttgcccact tccatacgtg tcctccttac cagaaattta 4441 tccttaaggt cgtcagctat cctgcaggcg atctctcgat ttcgatcaag acattccttt 4501 aatggtcttt tctggacacc actaggggtc agaagtagtt catcaaactt tcttccctcc 4561 ctaatctcat tggttacctt gggctatcga aacttaatta accagtcaag tcagctactt 4621 ggcgagatcg acttgtctgg gtttcgacta cgctcagaat tgcgtcagtc aagttcgatc 4681 tggtccttgc tattgcaccc gttctccgat tacgagtttc atttaaatca tgtgagcaaa 4741 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 4801 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 4861 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 4921 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 4981 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 5041 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 5101 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 5161 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 5221 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 5281 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 5341 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 5401 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 5461 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 5521 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 5581 agcgatctgt ctatttcgtt catccatagt tgcatttaaa tttccgaact ctccaaggcc 5641 ctcgtcggaa aatcttcaaa cctttcgtcc gatccatctt gcaggctacc tctcgaacga 5701 actatcgcaa gtctcttggc cggccttgcg ccttggctat tgcttggcag cgcctatcgc 5761 caggtattac tccaatcccg aatatccgag atcgggatca cccgagagaa gttcaaccta 5821 catcctcaat cccgatctat ccgagatccg aggaatatcg aaatcggggc gcgcctggcc 5881 tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc gtcacggcga gcgctgccac 5941 gtcagacgaa gggcgcagga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc 6001 cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg 6061 gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag 6121 tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta 6181 tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg 6241 ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg 6301 gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg 6361 gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca 6421 aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg 6481 ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag cccttcgcta 6541 atgcgggaaa gctcttattc gggtgagatg ggctgggcac catctgggga ccctgacgtg 6601 aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc 6661 ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg 6721 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag 6781 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag 6841 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta 6901 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg 6961 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt 7021 aattttcagt gttagacttg taaattgtcc gctaaattct ggccgttttt ggcttttttg 7081 ttagacaaca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 7141 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 7201 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 7261 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 7321 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 7381 ttgagcggtt cccggctggc cgcgcagcaa cagatggagg gcctcctggc gccgcaccgg 7441 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 7501 ggtctgggca gcgccgtcgt gctccccgga gtggaggccg ccgagcgcgc cggggtgccc 7561 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 7621 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 7681 ggtgcctagg ctagtatgta agcctagtct tagataataa aatcgctatc catcgaagat 7741 ggatgtgtgt tggttttttg tgtgtgtaac gctaggcgcg cctggtgtac cgagaacgat 7801 cctctcagtg cgagtctcga cgatccatat cgttgcttgg cagtcagcca gtcggaatcc 7861 agcttgggac ccaggaagtc caatcgtcag atattgtact caagcctggt cacggcagcg 7921 taccgatctg tttaaaccta gatattgata gtctgatcgg tcaacgtata atcgagtcct 7981 agcttttgca aacatctatc aagagacagg atcagcagga ggctttcgca tgagtattca 8041 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 8101 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcgc gagtgggtta 8161 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgctt 8221 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc 8281 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtattc 8341 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 8401 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgattg gaggaccgaa 8461 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 8521 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 8581 ggcaacaacc ttgcgtaaac tattaactgg cgaactactt actctagctt cccggcaaca 8641 gttgatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 8701 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 8761 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 8821 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 8881 gcattggtaa ccgattctag gtgcattggc gcagaaaaaa atgcctgatg cgacgctgcg 8941 cgtcttatac tcccacatat gccagattca gcaacggata cggcttcccc aacttgccca 9001 cttccatacg tgtcctcctt accagaaatt tatccttaag atcgtttaaa ctcgactctg 9061 gctctatcga atctccgtcg tttcgagctt acgcgaacag ccgtggcgct catttgctcg 9121 tcgggcatcg aatctcgtca gctatcgtca gcttaccttt ttggcagcga tcgcggctcc 9181 cgacatcttg gaccattagc tccacaggta tcttcttccc tctagtggtc ataacagcag 9241 cttcagctac ctctcaattc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt 9301 atgctatcaa tcgttgcgtt acacacacaa aaaaccaaca cacatccatc ttcgatggat 9361 agcgatttta ttatctaact gctgatcgag tgtagccaga tctcgtgagg ctccggtgcc 9421 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 9481 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 9541 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcact agtcgccgtg 9601 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc 9661 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg 9721 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgtgg 9781 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg tggcctggcc tgggcgctgg 9841 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct 9901 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt 9961 gtaaatgcgg gccaagatca gcacactggt atttcggttt ttggggccgc gggcggcgac 10021 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg 10081 agaatcggac gggggtagtc tcaagctgcc cggcctgctc tggtgcctgg cctcgcgccg 10141 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg 10201 gaaagatggc cgcttcccgg ccctgctgca gggagcacaa aatggaggac gcggcgctcg 10261 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc 10321 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctccagc 10381 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac 10441 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa 10501 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt 10561 ttttttcttc catttcaggt gtcgtgaaaa ctacccctaa aagccaaaag atctttgtcg 10621 atcctaccat ccactcgaca cacccgccag cggccgctgc caagcttccg agctctcgaa 10681 ttc //