LOCUS       Exported     4709 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4709
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     rep_origin      4059..4647
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     537..553
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    569..668
                     /note="Gateway cloning site"
                     /label="attL1"
     CDS             3175..3966
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             668..2588
                     /label="NSP2"
                     /note="Essential for viral replication. In Gateway cloning vector"
                     /translation="MAYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTCGYLPQNAVVKIYCPACHNSEVGPEHSLAEYHNESGLKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQKEKVNINIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLVNKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEIIFLEGETLPTEVLTEEVVLKTGDLQPLEQPTSEAVEAPLVGTPVCINGLMLLEIKDTEKYCALAPNMMVTNNTFTLKGG*"
     primer_bind     complement(2728..2744)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    complement(2589..2687)
                     /label="attL2"
                     /note="Gateway cloning site"
     promoter        complement(2705..2723)
                     /label="T7 promoter"
                     /note="t7 promoter"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGTTGGCATG GCTTACACCC GCTACGTGGA CAACAACTTC TGCGGTCCCG ACGGCTACCC
      721 TCTGGAATGC ATCAAGGACC TGCTGGCTAG AGCTGGCAAG GCTAGCTGCA CCCTGTCCGA
      781 GCAGCTGGAC TTCATCGACA CCAAGAGAGG AGTGTACTGC TGCCGTGAGC ACGAACACGA
      841 GATCGCTTGG TACACCGAAA GATCCGAGAA GTCTTACGAG CTGCAGACCC CATTCGAAAT
      901 CAAGCTGGCC AAGAAGTTCG ACACCTTCAA CGGCGAGTGC CCAAACTTCG TGTTCCCCCT
      961 GAACAGCATC ATCAAGACCA TCCAGCCCCG TGTGGAGAAG AAGAAGCTGG ACGGATTCAT
     1021 GGGTCGCATC CGCAGCGTGT ACCCAGTGGC TAGCCCCAAC GAATGCAACC AGATGTGCCT
     1081 GTCTACCCTG ATGAAGTGCG ACCACTGCGG AGAGACCAGC TGGCAGACCG GTGACTTCGT
     1141 GAAGGCTACC TGCGAATTCT GCGGAACCGA GAACCTGACC AAGGAAGGAG CTACCACCTG
     1201 TGGATACCTG CCACAGAACG CCGTGGTGAA GATCTACTGC CCCGCTTGCC ACAACTCCGA
     1261 AGTGGGCCCT GAGCACTCTC TGGCCGAATA CCACAACGAG TCCGGACTGA AGACCATCCT
     1321 GCGCAAGGGC GGAAGGACCA TCGCTTTCGG TGGCTGCGTG TTCTCTTACG TGGGCTGCCA
     1381 CAACAAGTGT GCTTACTGGG TGCCTAGAGC TTCCGCTAAC ATCGGTTGCA ACCACACCGG
     1441 CGTGGTGGGC GAAGGATCTG AGGGTCTGAA CGACAACCTG CTGGAGATCC TGCAGAAGGA
     1501 AAAGGTGAAC ATCAACATCG TGGGCGACTT CAAGCTGAAC GAGGAAATCG CCATCATCCT
     1561 GGCTAGCTTC TCCGCCAGCA CCAGCGCCTT CGTGGAAACC GTGAAGGGTC TGGACTACAA
     1621 GGCTTTCAAG CAGATCGTGG AGTCCTGCGG CAACTTCAAG GTGACCAAGG GAAAGGCCAA
     1681 GAAGGGTGCT TGGAACATCG GCGAGCAGAA GAGCATCCTG TCCCCACTGT ACGCCTTCGC
     1741 TTCTGAAGCC GCTAGAGTGG TGCGTTCTAT CTTCAGCAGA ACCCTGGAGA CCGCCCAGAA
     1801 CAGCGTGCGT GTGCTGCAGA AGGCCGCTAT CACCATCCTG GACGGAATCT CCCAGTACTC
     1861 TCTGCGCCTG ATCGACGCCA TGATGTTCAC CAGCGACCTG GCTACCAACA ACCTGGTGGT
     1921 CATGGCCTAC ATCACCGGAG GTGTGGTGCA GCTGACCTCC CAGTGGCTGA CCAACATCTT
     1981 CGGTACCGTG TACGAGAAGC TGAAGCCTGT GCTGGACTGG CTGGAGGAAA AGTTCAAGGA
     2041 AGGCGTGGAG TTCCTGAGGG ACGGATGGGA GATCGTGAAG TTCATCTCTA CCTGCGCTTG
     2101 CGAAATCGTG GGCGGACAGA TCGTGACCTG CGCCAAGGAA ATCAAGGAGA GCGTGCAGAC
     2161 CTTCTTCAAG CTGGTGAACA AGTTCCTGGC CCTGTGCGCT GACTCCATCA TCATCGGTGG
     2221 CGCCAAGCTG AAGGCTCTGA ACCTGGGAGA GACCTTCGTG ACCCACAGCA AGGGTCTGTA
     2281 CCGCAAGTGC GTGAAGTCCA GGGAGGAAAC CGGACTGCTG ATGCCTCTGA AGGCCCCAAA
     2341 GGAGATCATC TTCCTGGAAG GAGAGACCCT GCCTACCGAA GTGCTGACCG AGGAAGTGGT
     2401 GCTGAAGACT GGCGACCTGC AGCCACTGGA GCAGCCTACC TCCGAAGCCG TGGAGGCTCC
     2461 TCTGGTGGGT ACCCCAGTGT GCATCAACGG CCTGATGCTG CTGGAGATCA AGGACACCGA
     2521 AAAGTACTGC GCCCTGGCTC CAAACATGAT GGTGACCAAC AACACCTTCA CCCTGAAGGG
     2581 AGGTTAATAC CCAACTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT
     2641 TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTGCCA TCCAGCTGAT
     2701 ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG
     2761 TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG CCTCCTCTAG
     2821 ACCAGCCAGG ACAGAAATGC CTCGACTTCG CTGCTGCCCA AGGTTGCCGG GTGACGCACA
     2881 CCGTGGAAAC GGATGAAGGC ACGAACCCAG TGGACATAAG CCTGTTCGGT TCGTAAGCTG
     2941 TAATGCAAGT AGCGTATGCG CTCACGCAAC TGGTCCAGAA CCTTGACCGA ACGCAGCGGT
     3001 GGTAACGGCG CAGTGGCGGT TTTCATGGCT TGTTATGACT GTTTTTTTGG GGTACAGTCT
     3061 ATGCCTCGGG CATCCAAGCA GCAAGCGCGT TACGCCGTGG GTCGATGTTT GATGTTATGG
     3121 AGCAGCAACG ATGTTACGCA GCAGGGCAGT CGCCCTAAAA CAAAGTTAAA CATCATGAGG
     3181 GAAGCGGTGA TCGCCGAAGT ATCGACTCAA CTATCAGAGG TAGTTGGCGT CATCGAGCGC
     3241 CATCTCGAAC CGACGTTGCT GGCCGTACAT TTGTACGGCT CCGCAGTGGA TGGCGGCCTG
     3301 AAGCCACACA GTGATATTGA TTTGCTGGTT ACGGTGACCG TAAGGCTTGA TGAAACAACG
     3361 CGGCGAGCTT TGATCAACGA CCTTTTGGAA ACTTCGGCTT CCCCTGGAGA GAGCGAGATT
     3421 CTCCGCGCTG TAGAAGTCAC CATTGTTGTG CACGACGACA TCATTCCGTG GCGTTATCCA
     3481 GCTAAGCGCG AACTGCAATT TGGAGAATGG CAGCGCAATG ACATTCTTGC AGGTATCTTC
     3541 GAGCCAGCCA CGATCGACAT TGATCTGGCT ATCTTGCTGA CAAAAGCAAG AGAACATAGC
     3601 GTTGCCTTGG TAGGTCCAGC GGCGGAGGAA CTCTTTGATC CGGTTCCTGA ACAGGATCTA
     3661 TTTGAGGCGC TAAATGAAAC CTTAACGCTA TGGAACTCGC CGCCCGACTG GGCTGGCGAT
     3721 GAGCGAAATG TAGTGCTTAC GTTGTCCCGC ATTTGGTACA GCGCAGTAAC CGGCAAAATC
     3781 GCGCCGAAGG ATGTCGCTGC CGACTGGGCA ATGGAGCGCC TGCCGGCCCA GTATCAGCCC
     3841 GTCATACTTG AAGCTAGACA GGCTTATCTT GGACAAGAAG AAGATCGCTT GGCCTCGCGC
     3901 GCAGATCAGT TGGAAGAATT TGTCCACTAC GTGAAAGGCG AGATCACCAA GGTAGTCGGC
     3961 AAATAACCCT CGAGCCACCC ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC
     4021 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC
     4081 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT
     4141 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT
     4201 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT
     4261 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT
     4321 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG
     4381 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA
     4441 CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG
     4501 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG
     4561 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC
     4621 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
     4681 GCCTTTTGCT GGCCTTTTGC TCACATGTT
//