LOCUS       Exported     3713 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3713
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     rep_origin      3063..3651
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     537..553
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     protein_bind    569..668
                     /label="attL1"
                     /note="Gateway cloning site"
     CDS             2179..2970
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             668..1592
                     /label="NSP5"
                     /note="3CL-pro domain. In Gateway cloning vector"
                     /translation="MSGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ*"
     primer_bind     complement(1732..1748)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    complement(1593..1691)
                     /label="attL2"
                     /note="Gateway cloning site"
     promoter        complement(1709..1727)
                     /label="T7 promoter"
                     /note="t7 promoter"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGTTGGCATG TCCGGATTCC GCAAGATGGC CTTCCCTTCT GGCAAGGTGG AGGGCTGCAT
      721 GGTGCAGGTG ACCTGCGGAA CCACCACCCT GAACGGTCTG TGGCTGGACG ACGTGGTGTA
      781 CTGCCCCAGG CACGTGATCT GCACCTCTGA GGACATGCTG AACCCTAACT ACGAAGACCT
      841 GCTGATCAGA AAGAGCAACC ACAACTTCCT GGTGCAGGCC GGTAACGTGC AGCTGCGTGT
      901 GATCGGCCAC TCTATGCAGA ACTGCGTGCT GAAGCTGAAG GTGGACACCG CTAACCCCAA
      961 GACCCCTAAG TACAAGTTCG TGAGAATCCA GCCTGGTCAG ACCTTCTCCG TGCTGGCCTG
     1021 CTACAACGGA AGCCCATCCG GCGTGTACCA GTGCGCTATG CGTCCCAACT TCACCATCAA
     1081 GGGCAGCTTC CTGAACGGCT CTTGCGGAAG CGTGGGTTTC AACATCGACT ACGACTGCGT
     1141 GTCCTTCTGC TACATGCACC ACATGGAGCT GCCAACTGGA GTGCACGCTG GAACTGACCT
     1201 GGAGGGTAAC TTCTACGGCC CCTTCGTGGA CAGACAGACT GCTCAGGCTG CTGGCACCGA
     1261 CACCACCATC ACCGTGAACG TGCTGGCTTG GCTGTACGCC GCTGTGATCA ACGGAGACCG
     1321 CTGGTTCCTG AACAGGTTCA CCACCACCCT GAACGACTTC AACCTGGTGG CTATGAAGTA
     1381 CAACTACGAG CCTCTGACCC AGGACCACGT GGATATCCTG GGTCCACTGA GCGCCCAGAC
     1441 TGGAATCGCT GTGCTGGACA TGTGCGCCTC CCTGAAGGAA CTGCTGCAGA ACGGCATGAA
     1501 CGGACGCACC ATCCTGGGAT CCGCTCTGCT GGAGGACGAA TTCACCCCCT TCGACGTGGT
     1561 GAGGCAGTGC TCTGGTGTGA CCTTCCAGTA ATACCCAACT TTCTTGTACA AAGTTGGCAT
     1621 TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA
     1681 AATCATTATT GCCATCCAGC TGATATCCCC TATAGTGAGT CGTATTACAT GGTCATAGCT
     1741 GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA
     1801 AATATATCAT CATGCCTCCT CTAGACCAGC CAGGACAGAA ATGCCTCGAC TTCGCTGCTG
     1861 CCCAAGGTTG CCGGGTGACG CACACCGTGG AAACGGATGA AGGCACGAAC CCAGTGGACA
     1921 TAAGCCTGTT CGGTTCGTAA GCTGTAATGC AAGTAGCGTA TGCGCTCACG CAACTGGTCC
     1981 AGAACCTTGA CCGAACGCAG CGGTGGTAAC GGCGCAGTGG CGGTTTTCAT GGCTTGTTAT
     2041 GACTGTTTTT TTGGGGTACA GTCTATGCCT CGGGCATCCA AGCAGCAAGC GCGTTACGCC
     2101 GTGGGTCGAT GTTTGATGTT ATGGAGCAGC AACGATGTTA CGCAGCAGGG CAGTCGCCCT
     2161 AAAACAAAGT TAAACATCAT GAGGGAAGCG GTGATCGCCG AAGTATCGAC TCAACTATCA
     2221 GAGGTAGTTG GCGTCATCGA GCGCCATCTC GAACCGACGT TGCTGGCCGT ACATTTGTAC
     2281 GGCTCCGCAG TGGATGGCGG CCTGAAGCCA CACAGTGATA TTGATTTGCT GGTTACGGTG
     2341 ACCGTAAGGC TTGATGAAAC AACGCGGCGA GCTTTGATCA ACGACCTTTT GGAAACTTCG
     2401 GCTTCCCCTG GAGAGAGCGA GATTCTCCGC GCTGTAGAAG TCACCATTGT TGTGCACGAC
     2461 GACATCATTC CGTGGCGTTA TCCAGCTAAG CGCGAACTGC AATTTGGAGA ATGGCAGCGC
     2521 AATGACATTC TTGCAGGTAT CTTCGAGCCA GCCACGATCG ACATTGATCT GGCTATCTTG
     2581 CTGACAAAAG CAAGAGAACA TAGCGTTGCC TTGGTAGGTC CAGCGGCGGA GGAACTCTTT
     2641 GATCCGGTTC CTGAACAGGA TCTATTTGAG GCGCTAAATG AAACCTTAAC GCTATGGAAC
     2701 TCGCCGCCCG ACTGGGCTGG CGATGAGCGA AATGTAGTGC TTACGTTGTC CCGCATTTGG
     2761 TACAGCGCAG TAACCGGCAA AATCGCGCCG AAGGATGTCG CTGCCGACTG GGCAATGGAG
     2821 CGCCTGCCGG CCCAGTATCA GCCCGTCATA CTTGAAGCTA GACAGGCTTA TCTTGGACAA
     2881 GAAGAAGATC GCTTGGCCTC GCGCGCAGAT CAGTTGGAAG AATTTGTCCA CTACGTGAAA
     2941 GGCGAGATCA CCAAGGTAGT CGGCAAATAA CCCTCGAGCC ACCCATGACC AAAATCCCTT
     3001 AACGTGAGTT ACGCGTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT
     3061 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA
     3121 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC
     3181 TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC
     3241 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT
     3301 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT
     3361 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG
     3421 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA
     3481 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG
     3541 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA
     3601 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC
     3661 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT
//