LOCUS       Exported     3665 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3665
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     rep_origin      3015..3603
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     537..553
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    569..668
                     /label="attL1"
                     /note="Gateway cloning site"
     CDS             2131..2922
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             668..1544
                     /label="NSP6"
                     /note="Complex with NSP 3 & 4: DMV formation. In Gateway cloning vector"
                     /translation="MSAVKRTIKGTHHWLLLTILTSLLVLVQSTQWSLFFFLYENAFLPFAMGIIAMSAFAMMFVKHKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTARTVYDDGARRVWTLMNVLTLVYKVYYGNALDQAISMWALIISVTSNYSGVVTTVMFLARGIVFMCVEYCPIFFITGNTLQCIMLVYCFLGYFCTCYFGLFCLLNRYFRLTLGVYDYLVSTQEFRYMNSQGLLPPKNSIDAFKLNIKLLGVGGKPCIKVATVQ*"
     primer_bind     complement(1684..1700)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    complement(1545..1643)
                     /label="attL2"
                     /note="Gateway cloning site"
     promoter        complement(1661..1679)
                     /label="T7 promoter"
                     /note="t7 promoter"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGTTGGCATG AGCGCCGTGA AGAGAACCAT CAAGGGAACC CACCACTGGC TGCTGCTGAC
      721 CATCCTGACC TCCCTGCTGG TGCTGGTGCA GTCTACCCAG TGGAGCCTGT TCTTCTTCCT
      781 GTACGAGAAC GCCTTCCTGC CCTTCGCTAT GGGTATCATC GCCATGTCCG CCTTCGCTAT
      841 GATGTTCGTG AAGCACAAGC ACGCTTTCCT GTGCCTGTTC CTGCTGCCAT CCCTGGCCAC
      901 CGTGGCTTAC TTCAACATGG TGTACATGCC CGCCTCTTGG GTCATGCGCA TCATGACCTG
      961 GCTGGACATG GTGGACACCA GCCTGTCCGG CTTCAAGCTG AAGGACTGCG TGATGTACGC
     1021 CTCTGCTGTG GTGCTGCTGA TCCTGATGAC CGCTAGAACC GTGTACGATG ATGGTGCTAG
     1081 AAGGGTGTGG ACCCTGATGA ACGTGCTGAC CCTGGTGTAC AAGGTGTACT ACGGCAACGC
     1141 CCTGGACCAG GCTATCAGCA TGTGGGCTCT GATCATCTCT GTGACCAGCA ACTACTCCGG
     1201 AGTGGTGACC ACCGTGATGT TCCTGGCCAG AGGTATCGTG TTCATGTGCG TGGAGTACTG
     1261 CCCTATCTTC TTCATCACCG GCAACACCCT GCAGTGCATC ATGCTGGTGT ACTGCTTCCT
     1321 GGGTTACTTC TGCACCTGCT ATTTCGGCCT GTTCTGCCTG CTGAACAGAT ACTTCCGTCT
     1381 GACCCTGGGC GTGTACGACT ACCTGGTGTC CACCCAGGAA TTCCGTTACA TGAACTCTCA
     1441 GGGACTGCTG CCCCCTAAGA ACAGCATCGA CGCTTTCAAG CTGAACATCA AGCTGCTGGG
     1501 AGTGGGCGGA AAGCCATGCA TCAAGGTGGC CACCGTGCAG TAATACCCAA CTTTCTTGTA
     1561 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT
     1621 CAGTCAAAAT AAAATCATTA TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC
     1681 ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT
     1741 GCACAAGATA AAAATATATC ATCATGCCTC CTCTAGACCA GCCAGGACAG AAATGCCTCG
     1801 ACTTCGCTGC TGCCCAAGGT TGCCGGGTGA CGCACACCGT GGAAACGGAT GAAGGCACGA
     1861 ACCCAGTGGA CATAAGCCTG TTCGGTTCGT AAGCTGTAAT GCAAGTAGCG TATGCGCTCA
     1921 CGCAACTGGT CCAGAACCTT GACCGAACGC AGCGGTGGTA ACGGCGCAGT GGCGGTTTTC
     1981 ATGGCTTGTT ATGACTGTTT TTTTGGGGTA CAGTCTATGC CTCGGGCATC CAAGCAGCAA
     2041 GCGCGTTACG CCGTGGGTCG ATGTTTGATG TTATGGAGCA GCAACGATGT TACGCAGCAG
     2101 GGCAGTCGCC CTAAAACAAA GTTAAACATC ATGAGGGAAG CGGTGATCGC CGAAGTATCG
     2161 ACTCAACTAT CAGAGGTAGT TGGCGTCATC GAGCGCCATC TCGAACCGAC GTTGCTGGCC
     2221 GTACATTTGT ACGGCTCCGC AGTGGATGGC GGCCTGAAGC CACACAGTGA TATTGATTTG
     2281 CTGGTTACGG TGACCGTAAG GCTTGATGAA ACAACGCGGC GAGCTTTGAT CAACGACCTT
     2341 TTGGAAACTT CGGCTTCCCC TGGAGAGAGC GAGATTCTCC GCGCTGTAGA AGTCACCATT
     2401 GTTGTGCACG ACGACATCAT TCCGTGGCGT TATCCAGCTA AGCGCGAACT GCAATTTGGA
     2461 GAATGGCAGC GCAATGACAT TCTTGCAGGT ATCTTCGAGC CAGCCACGAT CGACATTGAT
     2521 CTGGCTATCT TGCTGACAAA AGCAAGAGAA CATAGCGTTG CCTTGGTAGG TCCAGCGGCG
     2581 GAGGAACTCT TTGATCCGGT TCCTGAACAG GATCTATTTG AGGCGCTAAA TGAAACCTTA
     2641 ACGCTATGGA ACTCGCCGCC CGACTGGGCT GGCGATGAGC GAAATGTAGT GCTTACGTTG
     2701 TCCCGCATTT GGTACAGCGC AGTAACCGGC AAAATCGCGC CGAAGGATGT CGCTGCCGAC
     2761 TGGGCAATGG AGCGCCTGCC GGCCCAGTAT CAGCCCGTCA TACTTGAAGC TAGACAGGCT
     2821 TATCTTGGAC AAGAAGAAGA TCGCTTGGCC TCGCGCGCAG ATCAGTTGGA AGAATTTGTC
     2881 CACTACGTGA AAGGCGAGAT CACCAAGGTA GTCGGCAAAT AACCCTCGAG CCACCCATGA
     2941 CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG
     3001 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA
     3061 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG
     3121 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG
     3181 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG
     3241 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA
     3301 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC
     3361 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC
     3421 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA
     3481 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT
     3541 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG
     3601 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC
     3661 ATGTT
//