LOCUS       Exported     3389 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3389
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     rep_origin      2739..3327
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     primer_bind     537..553
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    569..668
                     /label="attL1"
                     /note="Gateway cloning site"
     CDS             1855..2646
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             668..1268
                     /label="NSP8"
                     /note="Complex with NSP7: Primase. In Gateway cloning vector"
                     /translation="MAIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDADSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQ*"
     primer_bind     complement(1408..1424)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    complement(1269..1367)
                     /label="attL2"
                     /note="Gateway cloning site"
     promoter        complement(1385..1403)
                     /label="T7 promoter"
                     /note="t7 promoter"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGTTGGCATG GCCATCGCTA GCGAGTTCAG CTCCCTGCCT TCCTACGCCG CTTTCGCCAC
      721 CGCTCAGGAG GCTTACGAAC AGGCCGTGGC TAACGGCGAC TCCGAAGTGG TGCTGAAGAA
      781 GCTGAAGAAG TCCCTGAACG TGGCCAAGTC TGAGTTCGAC AGAGACGCCG CTATGCAGCG
      841 TAAGCTGGAA AAGATGGCCG ACCAGGCTAT GACCCAGATG TACAAGCAGG CTCGCTCTGA
      901 GGACAAGAGG GCTAAGGTGA CCAGCGCCAT GCAGACCATG CTGTTCACCA TGCTGCGCAA
      961 GCTGGACAAC GACGCTCTGA ACAACATCAT CAACAACGCC AGAGACGGCT GCGTGCCCCT
     1021 GAACATCATC CCTCTGACCA CCGCCGCTAA GCTGATGGTG GTCATCCCCG ACTACAACAC
     1081 CTACAAGAAC ACCTGCGACG GAACCACCTT CACCTACGCC TCCGCTCTGT GGGAGATCCA
     1141 GCAGGTGGTG GACGCTGACT CTAAGATCGT GCAGCTGTCT GAAATCAGCA TGGACAACAG
     1201 CCCAAACCTG GCTTGGCCAC TGATCGTGAC CGCCCTGCGC GCTAACTCTG CCGTGAAGCT
     1261 GCAGTAATAC CCAACTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT
     1321 TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTGCCA TCCAGCTGAT
     1381 ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG
     1441 TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG CCTCCTCTAG
     1501 ACCAGCCAGG ACAGAAATGC CTCGACTTCG CTGCTGCCCA AGGTTGCCGG GTGACGCACA
     1561 CCGTGGAAAC GGATGAAGGC ACGAACCCAG TGGACATAAG CCTGTTCGGT TCGTAAGCTG
     1621 TAATGCAAGT AGCGTATGCG CTCACGCAAC TGGTCCAGAA CCTTGACCGA ACGCAGCGGT
     1681 GGTAACGGCG CAGTGGCGGT TTTCATGGCT TGTTATGACT GTTTTTTTGG GGTACAGTCT
     1741 ATGCCTCGGG CATCCAAGCA GCAAGCGCGT TACGCCGTGG GTCGATGTTT GATGTTATGG
     1801 AGCAGCAACG ATGTTACGCA GCAGGGCAGT CGCCCTAAAA CAAAGTTAAA CATCATGAGG
     1861 GAAGCGGTGA TCGCCGAAGT ATCGACTCAA CTATCAGAGG TAGTTGGCGT CATCGAGCGC
     1921 CATCTCGAAC CGACGTTGCT GGCCGTACAT TTGTACGGCT CCGCAGTGGA TGGCGGCCTG
     1981 AAGCCACACA GTGATATTGA TTTGCTGGTT ACGGTGACCG TAAGGCTTGA TGAAACAACG
     2041 CGGCGAGCTT TGATCAACGA CCTTTTGGAA ACTTCGGCTT CCCCTGGAGA GAGCGAGATT
     2101 CTCCGCGCTG TAGAAGTCAC CATTGTTGTG CACGACGACA TCATTCCGTG GCGTTATCCA
     2161 GCTAAGCGCG AACTGCAATT TGGAGAATGG CAGCGCAATG ACATTCTTGC AGGTATCTTC
     2221 GAGCCAGCCA CGATCGACAT TGATCTGGCT ATCTTGCTGA CAAAAGCAAG AGAACATAGC
     2281 GTTGCCTTGG TAGGTCCAGC GGCGGAGGAA CTCTTTGATC CGGTTCCTGA ACAGGATCTA
     2341 TTTGAGGCGC TAAATGAAAC CTTAACGCTA TGGAACTCGC CGCCCGACTG GGCTGGCGAT
     2401 GAGCGAAATG TAGTGCTTAC GTTGTCCCGC ATTTGGTACA GCGCAGTAAC CGGCAAAATC
     2461 GCGCCGAAGG ATGTCGCTGC CGACTGGGCA ATGGAGCGCC TGCCGGCCCA GTATCAGCCC
     2521 GTCATACTTG AAGCTAGACA GGCTTATCTT GGACAAGAAG AAGATCGCTT GGCCTCGCGC
     2581 GCAGATCAGT TGGAAGAATT TGTCCACTAC GTGAAAGGCG AGATCACCAA GGTAGTCGGC
     2641 AAATAACCCT CGAGCCACCC ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC
     2701 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC
     2761 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT
     2821 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT
     2881 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT
     2941 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT
     3001 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG
     3061 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA
     3121 CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG
     3181 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG
     3241 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC
     3301 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
     3361 GCCTTTTGCT GGCCTTTTGC TCACATGTT
//