LOCUS       Exported                5550 bp ds-DNA     circular SYN 24-SEP-2020
DEFINITION  synthetic linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 24, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..5550
                     /note="""transcription terminator for bacteriophage T7 RNA"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 for
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /label=attL1
                     /note="Gateway cloning site"
     CDS             641..3427
                     /codon_start=1
                     /label=RNA-pol (NSP12)
                     /translation="MPTLYKKVGMSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFL
                     KTNCCRFQEKDEDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAVAKHDFFKFRIDG
                     DMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVE
                     NPDILRVYANLGERVRQALLKTVQFCDAMRNAGIVGVLTLDNQDLNGNWYDFGDFIQTT
                     PGSGVPVVDSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFDR
                     YFKYWDQTYHPNCVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVST
                     GYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSVAALTN
                     NVAFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPT
                     MCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFPFNKWGKARLYYDSMSY
                     EDQDALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIA
                     ATRGATVVIGTSKFYGGWHNMLKTVYSDVENPHLMGWDYPKCDRAMPNMLRIMASLVLA
                     RKHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNICQ
                     AVTANVNALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDTDFVNEFYAYLRKHFSMMI
                     LSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSEAKCWTETDLTKGPHEFCSQH
                     TMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQ
                     EYADVFHLYLQYIRKLHDELTGHMLDMYSVMLTNDNTSRYWEPEFYEAMYTPHTVLQ"
     protein_bind    complement(3429..3528)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(3546..3564)
                     /label=T7 promoter
                     /note="t7 promoter"
     primer_bind     complement(3569..3585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4016..4807
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      4900..5488
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg tccgccgcta gactgactcc ttgtggtact ggcacctcta ctgacgtggt
      721 gtacagggcc ttcgacatct acaacgacaa ggtggccgga ttcgctaagt tcctgaagac
      781 caactgctgc cgcttccagg agaaggacga agacgacaac ctgatcgact cttacttcgt
      841 ggtgaagagg cacaccttca gcaactacca gcacgaggaa accatctaca acctgctgaa
      901 ggactgcccc gccgtggcta agcacgactt cttcaagttc cgcatcgacg gtgacatggt
      961 gcctcacatc agccgccaga ggctgaccaa gtacaccatg gccgacctgg tgtacgctct
     1021 gaggcacttc gacgagggca actgcgacac cctgaaggaa atcctggtga cctacaactg
     1081 ctgcgacgac gactacttca acaagaagga ctggtacgac ttcgtggaga accctgacat
     1141 cctgagagtg tacgccaacc tgggcgaaag agtgcgtcag gctctgctga agaccgtgca
     1201 gttctgcgac gccatgcgta acgctggtat cgtgggcgtg ctgaccctgg acaaccagga
     1261 cctgaacggc aactggtacg acttcggaga cttcatccag actactccag gatctggtgt
     1321 gcctgtggtg gactcctact actctctgct gatgccaatc ctgaccctga ccagggccct
     1381 gaccgctgag tctcacgtgg acaccgacct gaccaagccc tacatcaagt gggacctgct
     1441 gaagtacgac ttcaccgagg aacgcctgaa gctgttcgac aggtacttca agtactggga
     1501 ccagacctac cacccaaact gcgtgaactg cctggacgac agatgcatcc tgcactgcgc
     1561 caacttcaac gtgctgttct ctaccgtgtt ccctcccacc agcttcggcc ctctggtgcg
     1621 taagatcttc gtggacggcg tgccattcgt ggtgtctacc ggataccact tcagagagct
     1681 gggtgtggtg cacaaccagg acgtgaacct gcacagctcc cgtctgagct tcaaggaact
     1741 gctggtgtac gctgctgacc ctgctatgca cgctgctagc ggcaacctgc tgctggacaa
     1801 gcgcaccacc tgcttctccg tggccgctct gaccaacaac gtggccttcc agaccgtgaa
     1861 gcctggaaac ttcaacaagg acttctacga cttcgctgtg agcaagggat tcttcaagga
     1921 gggttctagc gtggaactga agcacttctt cttcgcccag gacggcaacg ccgctatctc
     1981 cgactacgac tactaccgct acaacctgcc caccatgtgc gacatcaggc agctgctgtt
     2041 cgtggtggag gtggtggaca agtacttcga ctgctacgac ggcggatgca tcaacgctaa
     2101 ccaggtcatc gtgaacaacc tggacaagag cgccggcttc cctttcaaca agtggggaaa
     2161 ggctagactg tactacgaca gcatgtccta cgaagaccag gacgccctgt tcgcttacac
     2221 caagcgtaac gtgatcccaa ccatcaccca gatgaacctg aagtacgcca tctccgctaa
     2281 gaaccgcgcc aggaccgtgg ctggtgtgag catctgctcc accatgacca acagacagtt
     2341 ccaccagaag ctgctgaagt ctatcgccgc tacccgtggt gccaccgtgg tcatcggcac
     2401 cagcaagttc tacggtggct ggcacaacat gctgaagacc gtgtactccg acgtggagaa
     2461 cccacacctg atgggctggg actaccccaa gtgcgacaga gctatgccta acatgctgcg
     2521 tatcatggcc tctctggtgc tggctagaaa gcacaccacc tgctgctctc tgagccaccg
     2581 cttctacagg ctggccaacg agtgcgctca ggtgctgtcc gaaatggtca tgtgcggagg
     2641 ttctctgtac gtgaagcccg gcggaacctc ctctggagac gctaccaccg cctacgctaa
     2701 ctccgtgttc aacatctgcc aggccgtgac cgctaacgtg aacgccctgc tgtctaccga
     2761 cggtaacaag atcgctgaca agtacgtgag aaacctgcag caccgtctgt acgagtgcct
     2821 gtacagaaac cgtgacgtgg acaccgactt cgtgaacgaa ttctacgcct acctgcgcaa
     2881 gcacttctcc atgatgatcc tgtctgacga cgctgtggtg tgcttcaact ctacctacgc
     2941 cagccagggc ctggtggcta gcatcaagaa cttcaagtcc gtgctgtact accagaacaa
     3001 cgtgttcatg agcgaggcca agtgctggac cgaaaccgac ctgaccaagg gacctcacga
     3061 gttctgctcc cagcacacca tgctggtgaa gcagggtgac gactacgtgt acctgccata
     3121 ccctgaccct tctagaatcc tgggagctgg atgcttcgtg gacgacatcg tgaagaccga
     3181 cggcaccctg atgatcgaaa ggttcgtgag cctggccatc gacgcttacc ctctgaccaa
     3241 gcacccaaac caggagtacg ctgacgtgtt ccacctgtac ctgcagtaca tccgcaagct
     3301 gcacgacgaa ctgaccggac acatgctgga catgtacagc gtgatgctga ccaacgacaa
     3361 cacctccagg tactgggagc cagaattcta cgaggccatg tacacccctc acaccgtgct
     3421 gcagtaatac ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     3481 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     3541 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     3601 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     3661 gaccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac
     3721 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     3781 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     3841 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     3901 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     3961 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag
     4021 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     4081 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     4141 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     4201 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     4261 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     4321 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     4381 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     4441 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     4501 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     4561 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     4621 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     4681 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     4741 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     4801 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     4861 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4921 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4981 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5041 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5101 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5161 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5221 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5281 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5341 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5401 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5461 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5521 ggccttttgc tggccttttg ctcacatgtt
//
