LOCUS       Exported     4598 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4598
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     rep_origin      3948..4536
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     537..553
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    569..668
                     /note="Gateway cloning site"
                     /label="attL1"
     CDS             3064..3855
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /label="SmR"
     CDS             668..2477
                     /label="Heli (NSP13)"
                     /note="Helicase. In Gateway cloning vector"
                     /translation="MAVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCNAPGCDVTDVTQLYLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDFNAIATCDWTNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDRELHLSWEVGKPRPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVGDYFVLTSHTVMPLSAPTLVPQEHYVRITGLYPTLNISDEFSSNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARARVECFDKFKVNSTLEQYVFCTVNALPETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQLPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLKAHKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFISPYNSQNAVASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNVAITRAKVGILCIMSDRDLYDKLQFTSLEIPRRNVATLQ*"
     primer_bind     complement(2617..2633)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    complement(2478..2576)
                     /label="attL2"
                     /note="Gateway cloning site"
     promoter        complement(2594..2612)
                     /label="T7 promoter"
                     /note="t7 promoter"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGTTGGCATG GCCGTGGGCG CTTGCGTGCT GTGCAACTCT CAGACCAGCC TGAGATGTGG
      721 AGCTTGCATC CGCAGGCCTT TCCTGTGCTG CAAGTGCTGC TACGACCACG TGATCAGCAC
      781 CTCCCACAAG CTGGTGCTGT CCGTGAACCC ATACGTGTGC AACGCTCCTG GTTGTGACGT
      841 GACTGACGTG ACCCAGCTGT ACCTGGGCGG AATGAGCTAC TACTGCAAGT CCCACAAGCC
      901 CCCTATCAGC TTCCCACTGT GCGCTAACGG CCAGGTGTTC GGACTGTACA AGAACACCTG
      961 CGTGGGCTCC GACAACGTGA CCGACTTCAA CGCCATCGCT ACCTGCGACT GGACCAACGC
     1021 CGGAGACTAC ATCCTGGCTA ACACCTGCAC CGAAAGGCTG AAGCTGTTCG CCGCTGAGAC
     1081 CCTGAAGGCC ACCGAGGAAA CCTTCAAGCT GTCTTACGGT ATCGCTACCG TGCGTGAGGT
     1141 GCTGTCTGAC CGCGAACTGC ACCTGTCTTG GGAAGTGGGC AAGCCAAGAC CACCCCTGAA
     1201 CCGTAACTAC GTGTTCACCG GATACAGGGT GACCAAGAAC AGCAAGGTGC AGATCGGAGA
     1261 GTACACCTTC GAAAAGGGAG ACTACGGTGA CGCCGTGGTG TACAGAGGAA CCACCACCTA
     1321 CAAGCTGAAC GTGGGTGACT ACTTCGTGCT GACCTCCCAC ACCGTGATGC CACTGTCTGC
     1381 TCCCACCCTG GTGCCTCAGG AACACTACGT GCGCATCACC GGACTGTACC CAACCCTGAA
     1441 CATCTCCGAC GAGTTCAGCT CCAACGTGGC CAACTACCAG AAAGTGGGCA TGCAGAAGTA
     1501 CTCCACCCTG CAGGGACCTC CAGGTACCGG CAAGTCTCAC TTCGCCATCG GTCTGGCTCT
     1561 GTACTACCCC TCTGCCCGTA TCGTGTACAC TGCTTGTTCT CACGCTGCTG TGGATGCTCT
     1621 GTGCGAGAAG GCTCTGAAGT ACCTGCCTAT CGACAAGTGC TCCCGCATCA TCCCAGCCAG
     1681 AGCTCGTGTG GAATGCTTCG ACAAGTTCAA GGTGAACTCT ACCCTGGAGC AGTACGTGTT
     1741 CTGCACCGTG AACGCCCTGC CTGAAACCAC CGCTGACATC GTGGTGTTCG ACGAGATCAG
     1801 CATGGCCACC AACTACGACC TGTCTGTGGT GAACGCTAGA CTGAGGGCTA AGCACTACGT
     1861 GTACATCGGT GACCCAGCTC AGCTGCCTGC TCCTCGTACC CTGCTGACCA AGGGCACCCT
     1921 GGAGCCAGAA TACTTCAACA GCGTGTGCCG CCTGATGAAG ACCATCGGTC CAGACATGTT
     1981 CCTGGGTACC TGCCGCCGCT GCCCTGCTGA AATCGTGGAC ACCGTGTCTG CTCTGGTGTA
     2041 CGACAACAAG CTGAAGGCCC ACAAGGACAA GAGCGCCCAG TGCTTCAAGA TGTTCTACAA
     2101 GGGAGTGATC ACCCACGACG TGTCTAGCGC CATCAACAGG CCCCAGATCG GTGTGGTGCG
     2161 CGAGTTCCTG ACCAGGAACC CCGCCTGGAG AAAGGCCGTG TTCATCTCCC CTTACAACTC
     2221 TCAGAACGCC GTGGCTTCCA AGATCCTGGG TCTGCCTACC CAGACCGTGG ACTCCTCTCA
     2281 GGGCTCTGAA TACGACTACG TGATCTTCAC CCAGACCACC GAGACCGCCC ACAGCTGCAA
     2341 CGTGAACAGG TTCAACGTGG CCATCACCAG AGCTAAAGTG GGAATCCTGT GCATCATGAG
     2401 CGACAGAGAC CTGTACGACA AGCTGCAGTT CACCTCCCTG GAGATCCCCC GCAGGAACGT
     2461 GGCTACCCTG CAGTAATACC CAACTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG
     2521 CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTGCCAT
     2581 CCAGCTGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC
     2641 TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAAATAT ATCATCATGC
     2701 CTCCTCTAGA CCAGCCAGGA CAGAAATGCC TCGACTTCGC TGCTGCCCAA GGTTGCCGGG
     2761 TGACGCACAC CGTGGAAACG GATGAAGGCA CGAACCCAGT GGACATAAGC CTGTTCGGTT
     2821 CGTAAGCTGT AATGCAAGTA GCGTATGCGC TCACGCAACT GGTCCAGAAC CTTGACCGAA
     2881 CGCAGCGGTG GTAACGGCGC AGTGGCGGTT TTCATGGCTT GTTATGACTG TTTTTTTGGG
     2941 GTACAGTCTA TGCCTCGGGC ATCCAAGCAG CAAGCGCGTT ACGCCGTGGG TCGATGTTTG
     3001 ATGTTATGGA GCAGCAACGA TGTTACGCAG CAGGGCAGTC GCCCTAAAAC AAAGTTAAAC
     3061 ATCATGAGGG AAGCGGTGAT CGCCGAAGTA TCGACTCAAC TATCAGAGGT AGTTGGCGTC
     3121 ATCGAGCGCC ATCTCGAACC GACGTTGCTG GCCGTACATT TGTACGGCTC CGCAGTGGAT
     3181 GGCGGCCTGA AGCCACACAG TGATATTGAT TTGCTGGTTA CGGTGACCGT AAGGCTTGAT
     3241 GAAACAACGC GGCGAGCTTT GATCAACGAC CTTTTGGAAA CTTCGGCTTC CCCTGGAGAG
     3301 AGCGAGATTC TCCGCGCTGT AGAAGTCACC ATTGTTGTGC ACGACGACAT CATTCCGTGG
     3361 CGTTATCCAG CTAAGCGCGA ACTGCAATTT GGAGAATGGC AGCGCAATGA CATTCTTGCA
     3421 GGTATCTTCG AGCCAGCCAC GATCGACATT GATCTGGCTA TCTTGCTGAC AAAAGCAAGA
     3481 GAACATAGCG TTGCCTTGGT AGGTCCAGCG GCGGAGGAAC TCTTTGATCC GGTTCCTGAA
     3541 CAGGATCTAT TTGAGGCGCT AAATGAAACC TTAACGCTAT GGAACTCGCC GCCCGACTGG
     3601 GCTGGCGATG AGCGAAATGT AGTGCTTACG TTGTCCCGCA TTTGGTACAG CGCAGTAACC
     3661 GGCAAAATCG CGCCGAAGGA TGTCGCTGCC GACTGGGCAA TGGAGCGCCT GCCGGCCCAG
     3721 TATCAGCCCG TCATACTTGA AGCTAGACAG GCTTATCTTG GACAAGAAGA AGATCGCTTG
     3781 GCCTCGCGCG CAGATCAGTT GGAAGAATTT GTCCACTACG TGAAAGGCGA GATCACCAAG
     3841 GTAGTCGGCA AATAACCCTC GAGCCACCCA TGACCAAAAT CCCTTAACGT GAGTTACGCG
     3901 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT
     3961 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT
     4021 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG
     4081 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA
     4141 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT
     4201 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG
     4261 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG
     4321 AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC
     4381 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA
     4441 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT
     4501 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA
     4561 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTT
//