LOCUS       Exported                4377 bp ds-DNA     circular SYN 24-SEP-2020
DEFINITION  synthetic linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 24, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..4377
                     /label=T7 terminator
                     /note="""transcription terminator for bacteriophage T7 RNA"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 for
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /label=attL1
                     /note="Gateway cloning site"
     CDS             641..2254
                     /codon_start=1
                     /label=NSP14
                     /translation="MPTLYKKVGMAENVTGLFKDCSKVITGLHPTQAPTHLSVDTKFKT
                     EGLCVDIPGIPKDMTYRRLISMMGFKMNYQVNGYPNMFITREEAIRHVRAWIGFDVEGC
                     HATREAVGTNLPLQLGFSTGVNLVAVPTGYVDTPNNTDFSRVSAKPPPGDQFKHLIPLM
                     YKGLPWNVVRIKIVQMLSDTLKNLSDRVVFVLWAHGFELTSMKYFVKIGPERTCCLCDR
                     RATCFSTASDTYACWHHSIGFDYVYNPFMIDVQQWGFTGNLQSNHDLYCQVHGNAHVAS
                     CDAIMTRCLAVHECFVKRVDWTIEYPIIGDELKINAACRKVQHMVVKAALLADKFPVLH
                     DIGNPKAIKCVPQADVEWKFYDAQPCSDKAYKIEELFYSYATHSDKFTDGVCLFWNCNV
                     DRYPANSIVCRFDTRVLSNLNLPGCDGGSLYVNKHAFHTPAFDKSAFVNLKQLPFFYYS
                     DSPCESHGKQVVSDIDYVPLKSATCITRCNLGGAVCRHHANEYRLYLDAYNMMISAGFS
                     LWVYKQFDTYNLWNTFTRLQ"
     protein_bind    complement(2256..2355)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2373..2391)
                     /label=T7 promoter
                     /note="t7 promoter"
     primer_bind     complement(2396..2412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2843..3634
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      3727..4315
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gctgagaacg tgaccggtct gttcaaggac tgctctaagg tcatcactgg
      721 tctgcaccca actcaggctc caacccacct gtctgtggac accaagttca agaccgaagg
      781 actgtgcgtg gacatccccg gtatccctaa ggacatgacc taccgcaggc tgatctccat
      841 gatgggcttc aagatgaact accaggtgaa cggataccct aacatgttca tcaccagaga
      901 ggaagctatc cgccacgtga gggcctggat cggtttcgac gtggaaggtt gccacgctac
      961 cagagaagct gtgggtacca acctgccact gcagctgggc ttctctaccg gagtgaacct
     1021 ggtggctgtg cctaccggct acgtggacac cccaaacaac accgacttct ctagagtgag
     1081 cgccaagccc cctccaggag accagttcaa gcacctgatc cccctgatgt acaagggtct
     1141 gccttggaac gtggtgcgca tcaagatcgt gcagatgctg tccgacaccc tgaagaacct
     1201 gtctgacagg gtggtgttcg tgctgtgggc tcacggcttc gagctgacca gcatgaagta
     1261 cttcgtgaag atcggacccg aacgtacctg ctgcctgtgc gacagacgtg ccacctgctt
     1321 ctccaccgcc tctgacacct acgcttgctg gcaccactcc atcggattcg actacgtgta
     1381 caaccctttc atgatcgacg tgcagcagtg gggattcacc ggtaacctgc agtccaacca
     1441 cgacctgtac tgccaggtgc acggtaacgc ccacgtggct tcttgcgacg ctatcatgac
     1501 cagatgcctg gccgtgcacg agtgcttcgt gaagcgtgtg gactggacca tcgagtaccc
     1561 tatcatcggc gacgaactga agatcaacgc cgcttgcaga aaggtgcagc acatggtggt
     1621 gaaggccgct ctgctggctg acaagttccc agtgctgcac gacatcggaa acccaaaggc
     1681 tatcaagtgc gtgccccagg ccgacgtgga gtggaagttc tacgacgctc agccctgctc
     1741 cgacaaggcc tacaagatcg aggaactgtt ctactcttac gctacccaca gcgacaagtt
     1801 caccgacggc gtgtgcctgt tctggaactg caacgtggac aggtacccag ccaactctat
     1861 cgtgtgcaga ttcgacaccc gtgtgctgag caacctgaac ctgcccggat gcgacggcgg
     1921 atccctgtac gtgaacaagc acgccttcca cacccctgct ttcgacaagt ctgccttcgt
     1981 gaacctgaag cagctgcctt tcttctacta cagcgactcc ccatgcgaga gccacggcaa
     2041 gcaggtggtg tccgacatcg actacgtgcc cctgaagagc gccacctgca tcacccgctg
     2101 caacctgggc ggcgccgtgt gcagacacca cgccaacgaa tacaggctgt acctggacgc
     2161 ttacaacatg atgatcagcg ccggcttctc cctgtgggtg tacaagcagt tcgacaccta
     2221 caacctgtgg aacaccttca ccaggctgca gtaataccca actttcttgt acaaagttgg
     2281 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     2341 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     2401 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     2461 taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct
     2521 gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     2581 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     2641 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     2701 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     2761 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     2821 ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact
     2881 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     2941 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     3001 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     3061 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     3121 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     3181 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     3241 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     3301 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     3361 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     3421 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     3481 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     3541 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     3601 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc
     3661 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3721 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3781 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3841 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3901 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3961 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4021 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4081 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     4141 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4201 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4261 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4321 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
