LOCUS       Exported     3833 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3833
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     rep_origin      3183..3771
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     primer_bind     537..553
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    569..668
                     /label="attL1"
                     /note="Gateway cloning site"
     CDS             2299..3090
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             668..1712
                     /label="NSP15"
                     /note="XendoU: poly(U)-specific endoribonuclease. In Gateway cloning vector"
                     /translation="MSLENVAFNVVNKGHFDGQQGEVPVSIINNTVYTKVDGVDVELFENKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIAANTVIWDYKRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNARNGVLITEGSVKGLQPSVGPKQASLNGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQSRNLQEFKPRSQMEIDFLELAMDEFIERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGLAKRFKESPFELEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKVTIDYTEISFMLWCKDGHVETFYPKLQ*"
     primer_bind     complement(1852..1868)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    complement(1713..1811)
                     /label="attL2"
                     /note="Gateway cloning site"
     promoter        complement(1829..1847)
                     /label="T7 promoter"
                     /note="t7 promoter"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGTTGGCATG TCTCTGGAGA ACGTGGCCTT CAACGTGGTG AACAAGGGTC ACTTCGACGG
      721 TCAGCAGGGC GAAGTGCCTG TGAGCATCAT CAACAACACC GTGTACACCA AGGTGGACGG
      781 CGTGGACGTG GAGCTGTTCG AAAACAAGAC CACCCTGCCA GTGAACGTGG CCTTCGAGCT
      841 GTGGGCTAAG CGCAACATCA AGCCCGTGCC TGAAGTGAAG ATCCTGAACA ACCTGGGAGT
      901 GGACATCGCC GCTAACACCG TGATCTGGGA CTACAAGAGG GACGCCCCCG CTCACATCAG
      961 CACCATCGGC GTGTGCTCCA TGACCGACAT CGCCAAGAAG CCTACCGAGA CCATCTGCGC
     1021 TCCACTGACC GTGTTCTTCG ACGGAAGAGT GGACGGTCAG GTGGACCTGT TCCGCAACGC
     1081 TAGGAACGGC GTGCTGATCA CCGAGGGCTC TGTGAAGGGA CTGCAGCCAA GCGTGGGACC
     1141 CAAGCAGGCC TCCCTGAACG GTGTGACCCT GATCGGCGAA GCTGTGAAGA CCCAGTTCAA
     1201 CTACTACAAG AAGGTGGACG GCGTGGTGCA GCAGCTGCCA GAGACCTACT TCACCCAGTC
     1261 TAGAAACCTG CAGGAATTCA AGCCCCGTAG CCAGATGGAG ATCGACTTCC TGGAACTGGC
     1321 CATGGACGAG TTCATCGAAA GATACAAGCT GGAGGGATAC GCTTTCGAAC ACATCGTGTA
     1381 CGGTGACTTC TCTCACAGCC AGCTGGGCGG ACTGCACCTG CTGATCGGTC TGGCCAAGCG
     1441 TTTCAAGGAG TCCCCCTTCG AGCTGGAAGA CTTCATCCCT ATGGACTCTA CCGTGAAGAA
     1501 CTACTTCATC ACCGACGCTC AGACCGGAAG CTCCAAGTGC GTGTGCAGCG TGATCGACCT
     1561 GCTGCTGGAC GACTTCGTGG AGATCATCAA GTCCCAGGAC CTGTCCGTGG TGTCTAAGGT
     1621 GGTGAAGGTG ACCATCGACT ACACCGAGAT CTCCTTCATG CTGTGGTGCA AGGACGGCCA
     1681 CGTGGAAACC TTCTACCCTA AGCTGCAGTA ATACCCAACT TTCTTGTACA AAGTTGGCAT
     1741 TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA
     1801 AATCATTATT GCCATCCAGC TGATATCCCC TATAGTGAGT CGTATTACAT GGTCATAGCT
     1861 GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA
     1921 AATATATCAT CATGCCTCCT CTAGACCAGC CAGGACAGAA ATGCCTCGAC TTCGCTGCTG
     1981 CCCAAGGTTG CCGGGTGACG CACACCGTGG AAACGGATGA AGGCACGAAC CCAGTGGACA
     2041 TAAGCCTGTT CGGTTCGTAA GCTGTAATGC AAGTAGCGTA TGCGCTCACG CAACTGGTCC
     2101 AGAACCTTGA CCGAACGCAG CGGTGGTAAC GGCGCAGTGG CGGTTTTCAT GGCTTGTTAT
     2161 GACTGTTTTT TTGGGGTACA GTCTATGCCT CGGGCATCCA AGCAGCAAGC GCGTTACGCC
     2221 GTGGGTCGAT GTTTGATGTT ATGGAGCAGC AACGATGTTA CGCAGCAGGG CAGTCGCCCT
     2281 AAAACAAAGT TAAACATCAT GAGGGAAGCG GTGATCGCCG AAGTATCGAC TCAACTATCA
     2341 GAGGTAGTTG GCGTCATCGA GCGCCATCTC GAACCGACGT TGCTGGCCGT ACATTTGTAC
     2401 GGCTCCGCAG TGGATGGCGG CCTGAAGCCA CACAGTGATA TTGATTTGCT GGTTACGGTG
     2461 ACCGTAAGGC TTGATGAAAC AACGCGGCGA GCTTTGATCA ACGACCTTTT GGAAACTTCG
     2521 GCTTCCCCTG GAGAGAGCGA GATTCTCCGC GCTGTAGAAG TCACCATTGT TGTGCACGAC
     2581 GACATCATTC CGTGGCGTTA TCCAGCTAAG CGCGAACTGC AATTTGGAGA ATGGCAGCGC
     2641 AATGACATTC TTGCAGGTAT CTTCGAGCCA GCCACGATCG ACATTGATCT GGCTATCTTG
     2701 CTGACAAAAG CAAGAGAACA TAGCGTTGCC TTGGTAGGTC CAGCGGCGGA GGAACTCTTT
     2761 GATCCGGTTC CTGAACAGGA TCTATTTGAG GCGCTAAATG AAACCTTAAC GCTATGGAAC
     2821 TCGCCGCCCG ACTGGGCTGG CGATGAGCGA AATGTAGTGC TTACGTTGTC CCGCATTTGG
     2881 TACAGCGCAG TAACCGGCAA AATCGCGCCG AAGGATGTCG CTGCCGACTG GGCAATGGAG
     2941 CGCCTGCCGG CCCAGTATCA GCCCGTCATA CTTGAAGCTA GACAGGCTTA TCTTGGACAA
     3001 GAAGAAGATC GCTTGGCCTC GCGCGCAGAT CAGTTGGAAG AATTTGTCCA CTACGTGAAA
     3061 GGCGAGATCA CCAAGGTAGT CGGCAAATAA CCCTCGAGCC ACCCATGACC AAAATCCCTT
     3121 AACGTGAGTT ACGCGTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT
     3181 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA
     3241 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC
     3301 TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC
     3361 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT
     3421 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT
     3481 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG
     3541 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA
     3601 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG
     3661 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA
     3721 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC
     3781 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT
//