LOCUS       Exported     2963 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2963
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     rep_origin      2313..2901
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     537..553
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    569..668
                     /label="attL1"
                     /note="Gateway cloning site"
     CDS             1429..2220
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /label="SmR"
     CDS             668..842
                     /label="3b "
                     /note="Accessory protein - Activate inflammatory signal via RUNX1B, JNK and ERK. In Gateway cloning vector"
                     /translation="MAYCWRCTSCCFSERFQNHNPQKEMATSTLQGCSLCLQLAVVVCNSLLTPFARCCWP*"
     primer_bind     complement(982..998)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    complement(843..941)
                     /label="attL2"
                     /note="Gateway cloning site"
     promoter        complement(959..977)
                     /label="T7 promoter"
                     /note="t7 promoter"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGTTGGCATG GCCTACTGCT GGCGCTGCAC CAGCTGCTGC TTCTCCGAGA GGTTCCAGAA
      721 CCACAACCCC CAGAAGGAAA TGGCTACCTC TACCCTGCAG GGTTGTTCTC TGTGCCTGCA
      781 GCTGGCTGTG GTGGTGTGCA ACAGCCTGCT GACCCCCTTC GCTAGATGCT GCTGGCCTTA
      841 ATACCCAACT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG
      901 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT GCCATCCAGC TGATATCCCC
      961 TATAGTGAGT CGTATTACAT GGTCATAGCT GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA
     1021 AATCTCTGAT GTTACATTGC ACAAGATAAA AATATATCAT CATGCCTCCT CTAGACCAGC
     1081 CAGGACAGAA ATGCCTCGAC TTCGCTGCTG CCCAAGGTTG CCGGGTGACG CACACCGTGG
     1141 AAACGGATGA AGGCACGAAC CCAGTGGACA TAAGCCTGTT CGGTTCGTAA GCTGTAATGC
     1201 AAGTAGCGTA TGCGCTCACG CAACTGGTCC AGAACCTTGA CCGAACGCAG CGGTGGTAAC
     1261 GGCGCAGTGG CGGTTTTCAT GGCTTGTTAT GACTGTTTTT TTGGGGTACA GTCTATGCCT
     1321 CGGGCATCCA AGCAGCAAGC GCGTTACGCC GTGGGTCGAT GTTTGATGTT ATGGAGCAGC
     1381 AACGATGTTA CGCAGCAGGG CAGTCGCCCT AAAACAAAGT TAAACATCAT GAGGGAAGCG
     1441 GTGATCGCCG AAGTATCGAC TCAACTATCA GAGGTAGTTG GCGTCATCGA GCGCCATCTC
     1501 GAACCGACGT TGCTGGCCGT ACATTTGTAC GGCTCCGCAG TGGATGGCGG CCTGAAGCCA
     1561 CACAGTGATA TTGATTTGCT GGTTACGGTG ACCGTAAGGC TTGATGAAAC AACGCGGCGA
     1621 GCTTTGATCA ACGACCTTTT GGAAACTTCG GCTTCCCCTG GAGAGAGCGA GATTCTCCGC
     1681 GCTGTAGAAG TCACCATTGT TGTGCACGAC GACATCATTC CGTGGCGTTA TCCAGCTAAG
     1741 CGCGAACTGC AATTTGGAGA ATGGCAGCGC AATGACATTC TTGCAGGTAT CTTCGAGCCA
     1801 GCCACGATCG ACATTGATCT GGCTATCTTG CTGACAAAAG CAAGAGAACA TAGCGTTGCC
     1861 TTGGTAGGTC CAGCGGCGGA GGAACTCTTT GATCCGGTTC CTGAACAGGA TCTATTTGAG
     1921 GCGCTAAATG AAACCTTAAC GCTATGGAAC TCGCCGCCCG ACTGGGCTGG CGATGAGCGA
     1981 AATGTAGTGC TTACGTTGTC CCGCATTTGG TACAGCGCAG TAACCGGCAA AATCGCGCCG
     2041 AAGGATGTCG CTGCCGACTG GGCAATGGAG CGCCTGCCGG CCCAGTATCA GCCCGTCATA
     2101 CTTGAAGCTA GACAGGCTTA TCTTGGACAA GAAGAAGATC GCTTGGCCTC GCGCGCAGAT
     2161 CAGTTGGAAG AATTTGTCCA CTACGTGAAA GGCGAGATCA CCAAGGTAGT CGGCAAATAA
     2221 CCCTCGAGCC ACCCATGACC AAAATCCCTT AACGTGAGTT ACGCGTCGTT CCACTGAGCG
     2281 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC
     2341 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG
     2401 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC
     2461 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC
     2521 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC
     2581 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT
     2641 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT
     2701 GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC
     2761 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT
     2821 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA
     2881 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
     2941 TGCTGGCCTT TTGCTCACAT GTT
//