LOCUS       Exported     2921 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2921
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     rep_origin      2271..2859
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     537..553
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    569..668
                     /label="attL1"
                     /note="Gateway cloning site"
     CDS             1387..2178
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             668..800
                     /label="7b "
                     /note="Accessory protein - Induce inflammatory response. In Gateway cloning vector"
                     /translation="MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA"
     primer_bind     complement(940..956)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     protein_bind    complement(801..899)
                     /label="attL2"
                     /note="Gateway cloning site"
     promoter        complement(917..935)
                     /note="t7 promoter"
                     /label="T7 promoter"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGTTGGCATG ATCGAGCTGA GCCTGATCGA CTTCTACCTG TGCTTCCTGG CCTTCCTGCT
      721 GTTCCTGGTG CTGATCATGC TGATCATCTT CTGGTTCTCC CTGGAGCTGC AGGACCACAA
      781 CGAAACCTGC CACGCTTAAT ACCCAACTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA
      841 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTGC
      901 CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG
      961 CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA
     1021 TGCCTCCTCT AGACCAGCCA GGACAGAAAT GCCTCGACTT CGCTGCTGCC CAAGGTTGCC
     1081 GGGTGACGCA CACCGTGGAA ACGGATGAAG GCACGAACCC AGTGGACATA AGCCTGTTCG
     1141 GTTCGTAAGC TGTAATGCAA GTAGCGTATG CGCTCACGCA ACTGGTCCAG AACCTTGACC
     1201 GAACGCAGCG GTGGTAACGG CGCAGTGGCG GTTTTCATGG CTTGTTATGA CTGTTTTTTT
     1261 GGGGTACAGT CTATGCCTCG GGCATCCAAG CAGCAAGCGC GTTACGCCGT GGGTCGATGT
     1321 TTGATGTTAT GGAGCAGCAA CGATGTTACG CAGCAGGGCA GTCGCCCTAA AACAAAGTTA
     1381 AACATCATGA GGGAAGCGGT GATCGCCGAA GTATCGACTC AACTATCAGA GGTAGTTGGC
     1441 GTCATCGAGC GCCATCTCGA ACCGACGTTG CTGGCCGTAC ATTTGTACGG CTCCGCAGTG
     1501 GATGGCGGCC TGAAGCCACA CAGTGATATT GATTTGCTGG TTACGGTGAC CGTAAGGCTT
     1561 GATGAAACAA CGCGGCGAGC TTTGATCAAC GACCTTTTGG AAACTTCGGC TTCCCCTGGA
     1621 GAGAGCGAGA TTCTCCGCGC TGTAGAAGTC ACCATTGTTG TGCACGACGA CATCATTCCG
     1681 TGGCGTTATC CAGCTAAGCG CGAACTGCAA TTTGGAGAAT GGCAGCGCAA TGACATTCTT
     1741 GCAGGTATCT TCGAGCCAGC CACGATCGAC ATTGATCTGG CTATCTTGCT GACAAAAGCA
     1801 AGAGAACATA GCGTTGCCTT GGTAGGTCCA GCGGCGGAGG AACTCTTTGA TCCGGTTCCT
     1861 GAACAGGATC TATTTGAGGC GCTAAATGAA ACCTTAACGC TATGGAACTC GCCGCCCGAC
     1921 TGGGCTGGCG ATGAGCGAAA TGTAGTGCTT ACGTTGTCCC GCATTTGGTA CAGCGCAGTA
     1981 ACCGGCAAAA TCGCGCCGAA GGATGTCGCT GCCGACTGGG CAATGGAGCG CCTGCCGGCC
     2041 CAGTATCAGC CCGTCATACT TGAAGCTAGA CAGGCTTATC TTGGACAAGA AGAAGATCGC
     2101 TTGGCCTCGC GCGCAGATCA GTTGGAAGAA TTTGTCCACT ACGTGAAAGG CGAGATCACC
     2161 AAGGTAGTCG GCAAATAACC CTCGAGCCAC CCATGACCAA AATCCCTTAA CGTGAGTTAC
     2221 GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT
     2281 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT
     2341 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG
     2401 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT
     2461 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC
     2521 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG
     2581 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA
     2641 CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG
     2701 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG
     2761 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA
     2821 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT
     2881 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT T
//