LOCUS       Exported     3913 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3913
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     CDS             1..0
                     /label="NSP1 "
                     /note="Suppress antiviral host response by suppressing host gene expression. In Gateway cloning vector"
                     /translation="MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGG*"
     CDS             complement(1856..2389)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     CDS             complement(1..0)
                     /translation="MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGG*"
                     /label="NSP1 "
                     /note="Suppress antiviral host response by suppressing host gene expression. In Gateway cloning vector"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCC AGGAAGAGTT
       61 TGTAGAAACG CAAAAAGGCC ATCCGTCAGG ATGGCCTTCT GCTTAGTTTG ATGCCTGGCA
      121 GTTTATGGCG GGCGTCCTGC CCGCCACCCT CCGGGCCGTT GCTTCACAAC GTTCAAATCC
      181 GCTCCCGGCG GATTTGTCCT ACTCAGGAGA GCGTTCACCG ACAAACAACA GATAAAACGA
      241 AAGGCCCAGT CTTCCGACTG AGCCTTTCGT TTTATTTGAT GCCTGGCAGT TCCCTACTCT
      301 CGCGTTAACG CTAGCATGGA TCTCGGGCCC CAAATAATGA TTTTATTTTG ACTGATAGTG
      361 ACCTGTTCGT TGCAACAAAT TGATGAGCAA TGCTTTTTTA TAATGCCAAG TTTGTACAAA
      421 AAAGTTGGCA TGGAGAGCCT GGTGCCTGGC TTCAACGAAA AGACCCACGT GCAGCTGTCT
      481 CTGCCAGTGC TGCAGGTGCG CGACGTGCTG GTGAGGGGAT TCGGTGACTC TGTGGAGGAA
      541 GTGCTGTCTG AAGCTAGACA GCACCTGAAG GATGGTACCT GCGGACTGGT GGAGGTGGAA
      601 AAGGGCGTGC TGCCCCAGCT GGAGCAGCCT TACGTGTTCA TCAAGCGCAG CGATGCTAGG
      661 ACTGCTCCCC ACGGACACGT GATGGTGGAA CTGGTGGCCG AGCTGGAAGG TATCCAGTAC
      721 GGTCGCTCCG GAGAGACCCT GGGTGTGCTG GTGCCACACG TGGGTGAAAT CCCCGTGGCT
      781 TACAGAAAGG TGCTGCTGCG TAAGAACGGA AACAAGGGTG CCGGCGGACA CTCCTACGGT
      841 GCTGACCTGA AGTCTTTCGA CCTGGGCGAC GAGCTGGGAA CTGACCCTTA CGAGGACTTC
      901 CAGGAAAACT GGAACACCAA GCACAGCTCC GGAGTGACCA GAGAGCTGAT GCGTGAACTG
      961 AACGGTGGCT AATACCCAAC TTTCTTGTAC AAAGTGGGCA TTATAAGAAA GCATTGCTTA
     1021 TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA
     1081 GCTGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAAATAT
     1141 ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG
     1201 CCATATTCAA CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA
     1261 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA
     1321 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA
     1381 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT
     1441 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA
     1501 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT
     1561 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA
     1621 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG
     1681 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA
     1741 ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT
     1801 TATTTTTGAC GAGGGGAAAT TAGGCCGGGA AGCCGATCTC GGCTTGAACG AATTGTTAGG
     1861 TGGCGGTACT TGGGTCGATA TCAAAGTGCA TCACTTCTTC CCGTATGCCC AACTTTGTAT
     1921 AGAGAGCCAC TGCGGGATCG TCACCGTAAT CTGCTTGCAC GTAGATCACA TAAGCACCAA
     1981 GCGCGTTGGC CTCATGCTTG AGGAGATTGA TGAGCGCGGT GGCAATGCCC TGCCTCCGGT
     2041 GCTCGCCGGA GACTGCGAGA TCATAGATAT AGATCTCACT ACGCGGCTGC TCAAACCTGG
     2101 GCAGAACGTA AGCCGCGAGA GCGCCAACAA CCGCTTCTTG GTCGAAGGCA GCAAGCGCGA
     2161 TGAATGTCTT ACTACGGAGC AAGTTCCCGA GGTAATCGGA GTCCGGCTGA TGTTGGGAGT
     2221 AGGTGGCTAC GTCTCCGAAC TCACGACCGA AAAGATCAAG AGCAGCCCGC ATGGATTTGA
     2281 CTTGGTCAGG GCCGAGCCTA CATGTGCGAA TGATGCCCAT ACTTGAGCCA CCTAACTTTG
     2341 TTTTAGGGCG ACTGCCCTGC TGCGTAACAT CGTTGCTGCT GCGTAACATC GTTGCTGCTC
     2401 CATAACATCA AACATCGACC CACGGCGTAA CGCGCTTGCT GCTTGGATGC CCGAGGCATA
     2461 GACTGTACAA AAAAACAGTC ATAACAAGCC ATGAAAACCG CCACTGCGCC GTTACCACCG
     2521 CTGCGTTCGG TCAAGGTTCT GGACCAGTTG CGTGAGCGCA TACGCTACTT GCATTACAGT
     2581 TTACGAACCG AACAGGCTTA TGTCAACTGG GTTCGTGCCT TCATCCGTTT CCACGGTGTG
     2641 CGTCACCCGG CAACCTTGGG CAGCAGCGAA GTCGAGGCAT TTCTGTCCTG GCTGGCGAAC
     2701 GAGCGCAAGG TTTCGGTCTC CACGCATCGT CAGGCATTGG CGGCCTTGCT GTTCTTCTAC
     2761 GGCAAGGTGC TGTGCACGGA TCTGCCCTGG CTTCAGGAGA TCGGAAGACC TCGGCCGTCG
     2821 CGGCGCTTGC CGGTGGTGCT GACCCCGGAT GAAGTGGTTC GCATCCTCGG TTTTCTGGAA
     2881 GGCGAGCATC GTTTGTTCGC CCAGGACTCT AGCTATAGTT CTAGTGGTTG GCTACTAATA
     2941 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA
     3001 TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT
     3061 ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA
     3121 TCAGAATTGG TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC
     3181 GCAAGCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG
     3241 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC
     3301 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC
     3361 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT
     3421 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC
     3481 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT
     3541 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC
     3601 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG
     3661 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG
     3721 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG
     3781 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA
     3841 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT
     3901 TTGCTCACAT GTT
//