LOCUS       Exported                9211 bp ds-DNA     circular SYN 24-SEP-2020
DEFINITION  synthetic linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 24, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9211
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..9211
                     /label=T7 terminator
                     /note="""transcription terminator for bacteriophage T7 RNA"
     terminator      73..100
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      192..278
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             403..6270
                     /codon_start=1
                     /label=PLPRO (NSP3)
                     /translation="MPSLYKKVGMAPTKVTFGDDTVIEVQGYKSVNITFELDERIDKVL
                     NEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESG
                     EFKLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQP
                     EEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSG
                     YLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDD
                     YIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPL
                     LSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIP
                     KEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPD
                     SATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNY
                     ITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEE
                     TRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLINTLNDLNE
                     TLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFI
                     ETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLS
                     LREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYV
                     LPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATA
                     LLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQH
                     ANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYL
                     VQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKS
                     SEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLV
                     PNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKH
                     YTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSEDA
                     QGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGH
                     TDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVS
                     TTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFC
                     LEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREG
                     YLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWDLTAFGLVAEWF
                     LAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFF
                     ASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCK
                     LHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYF
                     DKAGQKTYERHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQL
                     MCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKN
                     VSLDNVLSTFISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVEN
                     MTPRDLGACIDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFK
                     LTCATTRQVVNVVTTKIALKGG"
     protein_bind    complement(6272..6371)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(7154..7687)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(7876..7904)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      8561..9149
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagttggca tggcccctac caaggtgacc ttcggagacg acaccgtgat cgaggtgcag
      481 ggttacaaga gcgtgaacat caccttcgag ctggacgaac gcatcgacaa ggtgctgaac
      541 gaaaagtgct ccgcttacac cgtggagctg ggcaccgagg tgaacgaatt cgcctgcgtg
      601 gtggccgacg ctgtgatcaa gaccctgcag ccagtgtccg aactgctgac ccctctgggt
      661 atcgacctgg acgagtggtc tatggctacc tactacctgt tcgacgagag cggcgaattc
      721 aagctggcct ctcacatgta ctgcagcttc taccctcccg acgaagacga ggaagagggt
      781 gactgcgaag aggaagagtt cgagccttcc actcagtacg agtacggcac cgaagacgac
      841 taccagggca agcctctgga attcggagcc acctctgccg ctctgcagcc tgaagaggaa
      901 caggaggaag actggctgga cgacgactcc cagcagaccg tgggtcagca ggacggctct
      961 gaagacaacc agaccaccac catccagacc atcgtggagg tgcagcctca gctggagatg
     1021 gaactgaccc cagtggtgca gaccatcgag gtgaacagct tctccggcta cctgaagctg
     1081 accgacaacg tgtacatcaa gaacgctgac atcgtggagg aagccaagaa ggtgaaacca
     1141 accgtggtgg tgaacgccgc taacgtgtac ctgaagcacg gcggaggtgt ggctggagct
     1201 ctgaacaagg ctaccaacaa cgccatgcag gtggaatccg acgactacat cgctaccaac
     1261 ggtcctctga aggtgggcgg atcctgcgtg ctgtctggcc acaacctggc caagcactgc
     1321 ctgcacgtgg tgggaccaaa cgtgaacaag ggagaggaca tccagctgct gaagagcgcc
     1381 tacgaaaact tcaaccagca cgaggtgctg ctggctccac tgctgtctgc cggaatcttc
     1441 ggtgctgatc ccatccacag cctgcgcgtg tgcgtggaca ccgtgaggac caacgtgtac
     1501 ctggccgtgt tcgacaagaa cctgtacgac aagctggtga gctccttcct ggagatgaag
     1561 tccgaaaagc aggtggagca gaagatcgcc gagatcccca aggaggaagt gaagcctttc
     1621 atcaccgaat ctaagcccag cgtggagcag agaaagcagg acgacaagaa gatcaaggcc
     1681 tgcgtggagg aagtgaccac caccctggag gaaaccaagt tcctgaccga gaacctgctg
     1741 ctgtacatcg acatcaacgg caacctgcac cctgactccg ctaccctggt gtctgacatc
     1801 gacatcacct tcctgaagaa ggacgccccc tacatcgtgg gcgacgtggt gcaggaagga
     1861 gtgctgaccg ctgtggtcat ccctaccaag aaggccggtg gcaccaccga gatgctggcc
     1921 aaggctctgc gtaaggtgcc aaccgacaac tacatcacca cctaccccgg tcagggcctg
     1981 aacggttaca ccgtggagga agctaagacc gtgctgaaga agtgcaagtc tgccttctac
     2041 atcctgccct ccatcatctc taacgagaag caggaaatcc tgggcaccgt gagctggaac
     2101 ctgcgcgaaa tgctggccca cgctgaggaa accaggaagc tgatgcccgt gtgcgtggag
     2161 accaaggcca tcgtgagcac catccagaga aagtacaagg gcatcaagat ccaggagggt
     2221 gtggtggact acggcgctcg tttctacttc tacacctcca agaccaccgt ggcctctctg
     2281 atcaacaccc tgaacgacct gaacgagacc ctggtgacca tgccactggg atacgtgacc
     2341 cacggtctga acctggagga agccgctaga tacatgcgtt ccctgaaggt gcctgctacc
     2401 gtgagcgtga gcagccctga cgctgtgacc gcctacaacg gatacctgac ctcctctagc
     2461 aagacccctg aggaacactt catcgaaacc atcagcctgg ccggttccta caaggactgg
     2521 tcttacagcg gacagtccac ccagctgggc atcgagttcc tgaagcgcgg agacaagagc
     2581 gtgtactaca cctccaaccc aaccaccttc cacctggacg gtgaagtgat caccttcgac
     2641 aacctgaaga ccctgctgtc tctgcgcgag gtgaggacca tcaaggtgtt caccaccgtg
     2701 gacaacatca acctgcacac ccaggtggtg gacatgagca tgacctacgg acagcagttc
     2761 ggtcctacct acctggacgg agctgacgtg accaagatca agccacacaa ctcccacgag
     2821 ggcaagacct tctacgtgct gcctaacgat gacaccctga gagtggaggc tttcgaatac
     2881 taccacacca ccgacccatc cttcctgggc aggtacatgt ctgccctgaa ccacaccaag
     2941 aagtggaagt acccccaggt gaacggactg accagcatca agtgggccga caacaactgc
     3001 tacctggcca ccgctctgct gaccctgcag cagatcgaac tgaagttcaa cccacccgct
     3061 ctgcaggacg cttactacag agcccgtgct ggcgaggccg ctaacttctg cgctctgatc
     3121 ctggcctact gcaacaagac cgtgggcgaa ctgggagacg tgagagagac catgagctac
     3181 ctgttccagc acgccaacct ggactcctgc aagcgtgtgc tgaacgtggt gtgcaagacc
     3241 tgcggccagc agcagaccac cctgaaggga gtggaagctg tgatgtacat gggtaccctg
     3301 tcctacgagc agttcaagaa gggcgtgcag atcccttgca cctgcggaaa gcaggccacc
     3361 aagtacctgg tgcagcagga aagcccattc gtgatgatgt ccgctcctcc agcccagtac
     3421 gaactgaagc acggcacctt cacctgcgct tccgagtaca ccggcaacta ccagtgcgga
     3481 cactacaagc acatcacctc taaggagacc ctgtactgca tcgacggagc cctgctgacc
     3541 aagtcctctg aatacaaggg tcccatcacc gacgtgttct acaaggagaa cagctacacc
     3601 accaccatca agcccgtgac ctacaagctg gacggcgtgg tgtgcaccga aatcgaccct
     3661 aagctggaca actactacaa gaaggacaac tcttacttca ccgagcagcc tatcgacctg
     3721 gtgccaaacc agccctaccc taacgccagc ttcgacaact tcaagttcgt gtgcgacaac
     3781 atcaagttcg ctgacgacct gaaccagctg accggataca agaagcctgc ctctagagag
     3841 ctgaaggtga ccttcttccc agacctgaac ggcgacgtgg tggctatcga ctacaagcac
     3901 tacaccccaa gcttcaagaa gggagccaag ctgctgcaca agcccatcgt gtggcacgtg
     3961 aacaacgcta ccaacaaggc cacctacaag cctaacacct ggtgcatccg ttgcctgtgg
     4021 tctaccaagc cagtggaaac ctccaactct ttcgacgtgc tgaagagcga ggacgctcag
     4081 ggcatggaca acctggcctg cgaagacctg aagcccgtga gcgaggaagt ggtggagaac
     4141 ccaaccatcc agaaggacgt gctggaatgc aacgtgaaga ccaccgaggt ggtgggagac
     4201 atcatcctga agccagctaa caacagcctg aagatcaccg aggaagtggg tcacaccgac
     4261 ctgatggccg cttacgtgga caacagctcc ctgaccatca agaagcccaa cgagctgagc
     4321 agggtgctgg gtctgaagac cctggctact cacggtctgg ctgctgtgaa ctctgtgcca
     4381 tgggacacca tcgctaacta cgccaagcct ttcctgaaca aggtggtgtc caccaccacc
     4441 aacatcgtga ccagatgtct gaaccgcgtg tgcaccaact acatgcccta cttcttcacc
     4501 ctgctgctgc agctgtgcac cttcaccaga agcaccaact cccgtatcaa ggctagcatg
     4561 cctaccacca tcgccaagaa caccgtgaag agcgtgggaa agttctgcct ggaggcttcc
     4621 ttcaactacc tgaagtctcc aaacttcagc aagctgatca acatcatcat ctggttcctg
     4681 ctgctctccg tgtgcctggg ttccctgatc tactctaccg ccgctctggg agtgctgatg
     4741 tctaacctgg gtatgcctag ctactgcact ggttacagag agggatacct gaacagcacc
     4801 aacgtgacca tcgccaccta ctgcaccggc agcatcccat gctccgtgtg cctgtctgga
     4861 ctggacagcc tggacaccta cccctccctg gaaaccatcc agatcaccat ctctagcttc
     4921 aagtgggacc tgaccgcctt cggtctggtg gctgagtggt tcctggccta catcctgttc
     4981 accaggttct tctacgtgct gggcctggcc gctatcatgc agctgttctt ctcctacttc
     5041 gctgtgcact tcatcagcaa ctcctggctg atgtggctga tcatcaacct ggtgcagatg
     5101 gctcccatct ctgccatggt gcgcatgtac atcttcttcg ccagcttcta ctacgtgtgg
     5161 aagtcctacg tgcacgtggt ggacggttgc aactcctcta cctgcatgat gtgctacaag
     5221 cgcaacaggg ctaccagggt ggagtgcacc accatcgtga acggcgtgcg caggtccttc
     5281 tacgtgtacg ccaacggcgg caagggattc tgcaagctgc acaactggaa ctgcgtgaac
     5341 tgcgacacct tctgcgctgg ctccaccttc atctctgacg aggtggccag agacctgagc
     5401 ctgcagttca agcgtccaat caaccccacc gaccagagct cctacatcgt ggactctgtg
     5461 accgtgaaga acggtagcat ccacctgtac ttcgacaagg ctggccagaa gacctacgag
     5521 cgccactctc tgagccactt cgtgaacctg gacaacctga gggccaacaa caccaagggc
     5581 agcctgccta tcaacgtgat cgtgttcgac ggaaagtcca agtgcgagga atctagcgcc
     5641 aagtccgcca gcgtgtacta cagccagctg atgtgccagc caatcctgct gctggaccag
     5701 gctctggtgt ctgacgtggg agacagcgcc gaagtggccg tgaagatgtt cgacgcctac
     5761 gtgaacacct tctcctctac cttcaacgtg cctatggaga agctgaagac cctggtggct
     5821 actgctgagg ctgaactggc taagaacgtg tccctggaca acgtgctgag caccttcatc
     5881 tccgccgcta ggcagggttt cgtggactct gacgtggaaa ccaaagacgt ggtggagtgc
     5941 ctgaagctgt ctcaccagag cgacatcgaa gtgaccggcg actcctgcaa caactacatg
     6001 ctgacctaca acaaggtgga gaacatgacc ccaagagacc tgggagcttg catcgactgc
     6061 tccgcccgtc acatcaacgc tcaggtggcc aagtctcaca acatcgccct gatctggaac
     6121 gtgaaggact tcatgagcct gtccgagcag ctgcgcaagc agatcaggag cgccgctaag
     6181 aagaacaacc tgcccttcaa gctgacctgc gctaccacca gacaggtggt gaacgtggtg
     6241 accaccaaga tcgccctgaa gggcggataa tacccaactt tcttgtacaa agtgggcatt
     6301 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     6361 atcattattt gccatccagc tgcagctctg gcccgtgtct caaaatctct gatgttacat
     6421 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     6481 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     6541 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     6601 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     6661 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     6721 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     6781 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     6841 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     6901 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     6961 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     7021 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     7081 tttctcactt gataacctta tttttgacga ggggaaatta ggccgggaag ccgatctcgg
     7141 cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc
     7201 gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt
     7261 agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg
     7321 caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac
     7381 gcggctgctc aaacctgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt
     7441 cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt
     7501 ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag
     7561 cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac
     7621 ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc
     7681 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
     7741 ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc
     7801 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     7861 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc
     7921 atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt
     7981 ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg
     8041 gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct tcaggagatc
     8101 ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc
     8161 atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag ctatagttct
     8221 agtggttggc tactaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     8281 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     8341 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     8401 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     8461 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8521 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8581 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8641 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8701 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8761 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8821 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8881 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8941 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9001 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9061 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9121 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9181 tggccttttg ctggcctttt gctcacatgt t
//
