LOCUS       Exported                4198 bp ds-DNA     circular SYN 24-SEP-2020
DEFINITION  synthetic linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 24, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..4198
                     /label=T7 terminator
                     /note="""transcription terminator for bacteriophage T7 RNA"
     terminator      73..100
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      192..278
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             403..1257
                     /codon_start=1
                     /label=3a
                     /translation="MPSLYKKVGMDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIP
                     IQASLPFGWLIVGVALLAVFQSASKIITLKKRWQLALSKGVHFVCNLLLLFVTVYSHLL
                     LVAAGLEAPFLYLYALVYFLQSINFVRIIMRLWLCWKCRSKNPLLYDANYFLCWHTNCY
                     DYCIPYNSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDCVVLHSYFTSDYYQ
                     LYSTQLSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDVSSGVVNPVMEPIYDEPTTTTS
                     VPL"
     protein_bind    complement(1259..1358)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2141..2674)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(2863..2891)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3548..4136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagttggca tggacctgtt catgagaatc ttcaccatcg gcaccgtgac cctgaagcag
      481 ggagagatca aggacgccac cccttctgac ttcgtgcgtg ccaccgctac catcccaatc
      541 caggctagcc tgcccttcgg atggctgatc gtgggtgtgg ccctgctggc cgtgttccag
      601 tccgcctcta agatcatcac cctgaagaag aggtggcagc tggctctgtc caagggtgtg
      661 cacttcgtgt gcaacctgct gctgctgttc gtgaccgtgt actcacacct gctgctggtg
      721 gctgctggtc tggaggctcc attcctgtac ctgtacgctc tggtgtactt cctgcagagc
      781 atcaacttcg tgcgcatcat catgaggctg tggctgtgct ggaagtgcag atccaagaac
      841 cccctgctgt acgacgccaa ctacttcctg tgctggcaca ccaactgcta cgactactgc
      901 atcccctaca acagcgtgac cagctccatc gtgatcacct ccggtgacgg caccaccagc
      961 cctatctccg aacacgacta ccagatcggc ggatacaccg agaagtggga atctggagtg
     1021 aaggactgcg tggtgctgca ctcttacttc accagcgact actaccagct gtactccacc
     1081 cagctgtcta ccgacaccgg tgtggagcac gtgaccttct tcatctacaa caagatcgtg
     1141 gacgaacccg aggaacacgt gcagatccac accatcgacg tgtctagcgg cgtggtgaac
     1201 cccgtgatgg agcctatcta cgacgaacct accaccacca cctccgtgcc actgtaatac
     1261 ccaactttct tgtacaaagt gggcattata agaaagcatt gcttatcaat ttgttgcaac
     1321 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc
     1381 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     1441 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     1501 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     1561 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     1621 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     1681 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     1741 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     1801 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     1861 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     1921 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     1981 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     2041 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2101 gaaattaggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt
     2161 cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg
     2221 gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat
     2281 gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg
     2341 cgagatcata gatatagatc tcactacgcg gctgctcaaa cctgggcaga acgtaagccg
     2401 cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac
     2461 ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc
     2521 cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga
     2581 gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc
     2641 cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc tgctccataa catcaaacat
     2701 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa
     2761 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     2821 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag
     2881 gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     2941 ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg
     3001 gtctccacgc atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc
     3061 acggatctgc cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg
     3121 gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg
     3181 ttcgcccagg actctagcta tagttctagt ggttggctac taataggttg tattgatgtt
     3241 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     3301 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     3361 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     3421 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     3481 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3541 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3601 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3661 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3721 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3781 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3841 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3901 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3961 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4021 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4081 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4141 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
