LOCUS       Exported                3598 bp ds-DNA     circular SYN 24-SEP-2020
DEFINITION  synthetic linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 24, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..3598
                     /label=T7 terminator
                     /note="""transcription terminator for bacteriophage T7 RNA"
     terminator      73..100
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      192..278
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             403..657
                     /codon_start=1
                     /label=E
                     /translation="MPSLYKKVGMYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAILTA
                     LRLCAYCCNIVNVSLVKPSFYVYSRVKNLNSSRVPDLLV"
     protein_bind    complement(659..758)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1541..2074)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(2263..2291)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      2948..3536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagttggca tgtactcttt cgtgagcgag gaaaccggca ccctgatcgt gaactccgtg
      481 ctgctgttcc tggccttcgt ggtgttcctg ctggtgaccc tggctatcct gaccgctctg
      541 agactgtgcg cttactgctg caacatcgtg aacgtgtccc tggtgaagcc ctctttctac
      601 gtgtacagcc gcgtgaagaa cctgaacagc tccagggtgc ctgacctgct ggtgtaatac
      661 ccaactttct tgtacaaagt gggcattata agaaagcatt gcttatcaat ttgttgcaac
      721 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc
      781 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
      841 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
      901 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
      961 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     1021 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     1081 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     1141 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     1201 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     1261 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     1321 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     1381 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     1441 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     1501 gaaattaggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt
     1561 cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg
     1621 gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat
     1681 gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg
     1741 cgagatcata gatatagatc tcactacgcg gctgctcaaa cctgggcaga acgtaagccg
     1801 cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac
     1861 ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc
     1921 cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga
     1981 gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc
     2041 cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc tgctccataa catcaaacat
     2101 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa
     2161 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     2221 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag
     2281 gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     2341 ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg
     2401 gtctccacgc atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc
     2461 acggatctgc cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg
     2521 gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg
     2581 ttcgcccagg actctagcta tagttctagt ggttggctac taataggttg tattgatgtt
     2641 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2701 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     2761 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     2821 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     2881 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2941 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3001 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3061 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3121 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3181 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3241 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3301 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3361 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3421 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3481 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3541 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
