LOCUS       Exported                4039 bp ds-DNA     circular SYN 24-SEP-2020
DEFINITION  synthetic linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 24, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..4039
                     /label=T7 terminator
                     /note="""transcription terminator for bacteriophage T7 RNA"
     terminator      73..100
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      192..278
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             403..1098
                     /codon_start=1
                     /label=M
                     /translation="MPSLYKKVGMADSNGTITVEELKKLLEQWNLVIGFLFLTWICLLQ
                     FAYANRNRFLYIIKLIFLWLLWPVTLACFVLAAVYRINWITGGIAIAMACLVGLMWLSY
                     FIASFRLFARTRSMWSFNPETNILLNVPLHGTILTRPLLESELVIGAVILRGHLRIAGH
                     HLGRCDIKDLPKEITVATSRTLSYYKLGASQRVAGDSGFAAYSRYRIGNYKLNTDHSSS
                     SDNIALLVQ"
     protein_bind    complement(1100..1199)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1982..2515)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(2704..2732)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3389..3977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagttggca tggctgactc taacggtacc atcaccgtgg aggaactgaa gaagctgctg
      481 gagcagtgga acctggtcat cggcttcctg ttcctgacct ggatctgcct gctgcagttc
      541 gcctacgcta accgcaacag gttcctgtac atcatcaagc tgatcttcct gtggctgctg
      601 tggcctgtga ccctggcttg cttcgtgctg gctgccgtgt accgcatcaa ctggatcacc
      661 ggcggaatcg ccatcgctat ggcctgcctg gtgggcctga tgtggctgtc ttacttcatc
      721 gctagcttca ggctgttcgc cagaacccgt tccatgtggt ctttcaaccc cgagaccaac
      781 atcctgctga acgtgcctct gcacggaacc atcctgacca gaccactgct ggagagcgaa
      841 ctggtcatcg gcgctgtgat cctgagagga cacctgcgta tcgccggaca ccacctgggt
      901 cgttgcgaca tcaaggacct gcccaaggaa atcaccgtgg ctaccagccg caccctgtcc
      961 tactacaagc tgggagcttc tcagagagtg gctggtgact ctggtttcgc tgcttactct
     1021 cgctacagga tcggtaacta caagctgaac accgaccaca gctcctctag cgacaacatc
     1081 gccctgctgg tgcagtaata cccaactttc ttgtacaaag tgggcattat aagaaagcat
     1141 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     1201 catccagctg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     1261 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     1321 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     1381 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     1441 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     1501 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     1561 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     1621 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     1681 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     1741 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     1801 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     1861 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     1921 taaccttatt tttgacgagg ggaaattagg ccgggaagcc gatctcggct tgaacgaatt
     1981 gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact
     2041 ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag
     2101 caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc
     2161 tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa
     2221 acctgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa
     2281 gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt
     2341 gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg
     2401 atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta
     2461 actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg
     2521 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
     2581 ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     2641 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     2701 tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac
     2761 ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg
     2821 gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc
     2881 ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg
     2941 ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt
     3001 ctggaaggcg agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta
     3061 ctaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     3121 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     3181 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     3241 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     3301 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3361 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3421 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3481 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3541 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3601 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3661 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3721 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3781 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3841 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3901 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3961 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4021 ggccttttgc tcacatgtt
//
