LOCUS       Exported                3736 bp ds-DNA     circular SYN 24-SEP-2020
DEFINITION  synthetic linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 24, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..3736
                     /note="""transcription terminator for bacteriophage T7 RNA"
     terminator      73..100
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      192..278
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             403..795
                     /codon_start=1
                     /label=7a
                     /translation="MPSLYKKVGMKIILFLALITLATCELYHYQECVRGTTVLLKEPCS
                     SGTYEGNSPFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRARSVSPKLFIRQEEVQE
                     LYSPIFLIVAAIVFITLCFTLKRKTE"
     protein_bind    complement(797..896)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1679..2212)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(2401..2429)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3086..3674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagttggca tgaagatcat cctgttcctg gccctgatca ccctggctac ctgcgagctg
      481 taccactacc aggaatgcgt gcgcggtacc accgtgctgc tgaaggagcc ttgcagctcc
      541 ggcacctacg aaggaaactc ccccttccac cctctggccg acaacaagtt cgctctgacc
      601 tgcttctcta ctcagttcgc cttcgcttgc cctgatggtg tgaagcacgt gtaccagctg
      661 cgcgccaggt ctgtgagccc aaagctgttc atcagacagg aggaagtgca ggagctgtac
      721 agccccatct tcctgatcgt ggccgctatc gtgttcatca ccctgtgctt caccctgaag
      781 cgtaagaccg aataataccc aactttcttg tacaaagtgg gcattataag aaagcattgc
      841 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
      901 ccagctgcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
      961 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     1021 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     1081 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     1141 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     1201 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     1261 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     1321 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     1381 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     1441 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     1501 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     1561 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     1621 ccttattttt gacgagggga aattaggccg ggaagccgat ctcggcttga acgaattgtt
     1681 aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg cccaactttg
     1741 tatagagagc cactgcggga tcgtcaccgt aatctgcttg cacgtagatc acataagcac
     1801 caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc
     1861 ggtgctcgcc ggagactgcg agatcataga tatagatctc actacgcggc tgctcaaacc
     1921 tgggcagaac gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg
     1981 cgatgaatgt cttactacgg agcaagttcc cgaggtaatc ggagtccggc tgatgttggg
     2041 agtaggtggc tacgtctccg aactcacgac cgaaaagatc aagagcagcc cgcatggatt
     2101 tgacttggtc agggccgagc ctacatgtgc gaatgatgcc catacttgag ccacctaact
     2161 ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg
     2221 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     2281 atagactgta caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     2341 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
     2401 agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg tttccacggt
     2461 gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg
     2521 aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc
     2581 tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag acctcggccg
     2641 tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct cggttttctg
     2701 gaaggcgagc atcgtttgtt cgcccaggac tctagctata gttctagtgg ttggctacta
     2761 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2821 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     2881 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     2941 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     3001 ggcgcaagct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3061 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     3241 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3721 cttttgctca catgtt
//
