LOCUS       BBF10K_003569           6787 bp ds-DNA     circular     18-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     source          1..6787
                     /label="source"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      38..85
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    265..515
                     /label="incC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(518..1273)
                     /label="repE"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     exon            complement(518..1273)
                     /label="Translation 1273-518"
     rep_origin      1364..1583
                     /label="ori2"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    2003..2024
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        2039..2069
                     /label="lac promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    2077..2093
                     /label="lac operator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     2101..2117
                     /label="M13 rev"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2190..3035
                     /label="repL"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     exon            2190..3035
                     /label="Translation 2190-3035"
     misc_feature    3143..3294
                     /label="nnpp promoters"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             3334..4149
                     /label="KanR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     exon            3334..4149
                     /label="Translation 3334-4149"
     terminator      complement(4259..4302)
                     /label="bacterial terminator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     4320..4336
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      4337..4368
                     /label="tonB terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4389..6787
                     /label="EcRNAseH"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc
       61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt
      121 gtcgcgtatg catcgatcac gtgtccacgt tctttaatag tggactcttg ttccaaactg
      181 gaacaacact cggatcgatc cggcgcgcac cgtgggaaaa actccaggta gaggtacaca
      241 cgcggatagc caattcagag taataaactg tgataatcaa ccctcatcaa tgatgacgaa
      301 ctaacccccg atatcaggtc acatgacgaa gggaaagaga aggaaatcaa ctgtgacaaa
      361 ctgccctcaa atttggcttc cttaaaaatt acagttcaaa aagtatgaga aaatccatgc
      421 aggctgaagg aaacagcaaa actgtgacaa attaccctca gtaggtcaga acaaatgtga
      481 cgaaccaccc tcaaatctgt gacagataac cctcagacta tcctgtcgtc atggaagtga
      541 tatcgcggaa ggaaaatacg atatgagtcg tctggcggcc tttctttttc tcaatgtatg
      601 agaggcgcat tggagttctg ctgttgatct cattaacaca gacttgcagg aagcggcggc
      661 ggaagtcagg catacgctgg taactttgag gcagctggta acgctctatg atccagtcga
      721 ttttcagaga cacgatgcct gagccatccg gcttacgata ctgacacagg gattcgtata
      781 aacgcatggc atacggattg gtgatttctt ttgtttcact aagccgaaac tgcgtaaacc
      841 ggttctgtaa cccgataaag aagggaatga gatatgggtt gatatgtaca ctgtaaagcc
      901 ctctggatgg actgtgcgca cgtttgataa accaaggaaa agattcatag cctttttcat
      961 caccggcatc ctcttcaggg cgataaaaaa ccacttcctt ccccgcgaaa ctcttcaatg
     1021 cctgccgtat atccttactg gcttccgcag aggtcaatcc gaatatttca gcatatttag
     1081 caacatggat ctcgcagata ccgtcatgtt cctgtagggt gccatcagat tttctgatct
     1141 ggtcaacgaa cagatacagc atacgttttt gatcccggga gagactatat gccgcctcag
     1201 tgaggtcgtt tgactggacg attcgcgggc tatttttacg tttcttgtga ttgataaccg
     1261 ctgtttccgc catgacagat ccatgtgaag tgtgacaagt ttttagattg tcacactaaa
     1321 taaaaaagag tcaataagca gggataactt tgtgaaaaaa cagcttcttc tgagggcaat
     1381 ttgtcacagg gttaagggca atttgtcaca gacaggactg tcatttgagg gtgatttgtc
     1441 acactgaaag ggcaatttgt cacaacacct tctgtagaac cagcatggat aaaggcctac
     1501 aaggcgctct aaaaaagaag atctaaaaac tataaaaaaa ataattataa aaatatcccc
     1561 gtggataagt ggataacccc aagggaagtt ttttcaggca tcgtgtgtaa gcagaatata
     1621 taagtgctgt tccctggtgc ttcctcgctc actcgaaatt cccggggata gctttatgct
     1681 tgtaaaccgt tttgtgaaaa aatttttaaa ataaaaaagg ggacctctag ggtccccaat
     1741 taattagtaa tataatctat taaaggtcat tcaaaaggtc atccaccgga tcaattcccc
     1801 tgctcgcgca ggctgggtgc caagctctcg ggtaacatca aggcccgatc cttggagccc
     1861 ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca gatccttgac ccgcagttgc
     1921 aaaccctcac tgatccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1981 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     2041 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     2101 caggaaacag ctatgaccat gattacgcca agcgcggtac ctgtcgcggc aacgcgctaa
     2161 cagacgtagt aagaaccacc agcattgtaa tgctggctaa agtcactttc ctgagctgta
     2221 taacgatgag cgattttact ttttctggct atgaattggc ctgctttgta acacactccg
     2281 gtctatcccg tagcgccggg catatcctgt cgcaatgtgc aaatctcgcg gcaacaacca
     2341 gtgaatactt cattcacaag cctcaccgcc tgatcgcggc agaaactggt tatagccaat
     2401 caaccgtcgt tcgtgcattc cgtgaagctg taaacaaagg tatcctgtct gtagagattg
     2461 ttatcggcga tcaccgtgaa cgtcgcgcta acctgtaccg gtttacacca tcctttttgg
     2521 ccttcgcaca acaagccaaa aatgcgctga tagaaagcaa attaaagatc tcttcagcgg
     2581 caaccaaggt taaagctgtt ctcgctaaga cattggcttt atttaatttt ttatccacac
     2641 ccccatgtca aaatgatacc ccctccccct gtcaggatga cgtggcaata aagaataaga
     2701 agtcacaagt taaaaaaaca aaaagatcag tttccggcgg tgccggaaca accagcctca
     2761 aaaaattgac ttcatggatc gctaaggcaa aagcaaaggc tgacaatctg cggttatcca
     2821 aaaaacgcac tcaaaaacat gagttcaagc agaaagtaga ggcggctgcg cggaaatatg
     2881 cttacctgaa gaacaagcgt tcgcctgata ttggcgggat atcaaacttc gataacctac
     2941 cgcattgcat gacggtaaac gaagctctta atgcggtttt agccaaaaat aaagataacg
     3001 aacaatgggg tataccggca ggattcagag ggtaatgaat tgctctaatt ataaccatgc
     3061 atactttcaa cacctctagt ttgccatgag gcaaactcat aggtgtcctg gtaagaggac
     3121 actgttgcca aaactggacg ccccattatt gcaattaata aacaactaac ggacaattct
     3181 acctaacaat aagtggagtt gcggccggcc gcgatccgct agcaaagcca cgttgtgtct
     3241 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     3301 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
     3361 tgctcgcgcc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     3421 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     3481 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     3541 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     3601 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta
     3661 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     3721 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgcctc
     3781 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3841 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactgtt gccattctca
     3901 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3961 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     4021 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     4081 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     4141 tttttctaat cagaattggt taattggttg taacactggc gagctctgcc acctgacgtc
     4201 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     4261 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctgcacctg caccagtcag
     4321 taaaacgacg gccagtagtc aaaagcctcc gaccggaggc ttttgacttg gttcaggtgg
     4381 agtgggagTT AATTAACATT ACTCGCATCC ATTCTCAGGC TGTCTCGTCT CGTCTCACGA
     4441 TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG
     4501 GTCGAGATCC CGGTGCCTAA TGAGTGAGCT AACTTACATT AATTGCGTTG CGCTCACTGC
     4561 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG
     4621 GGAGAGGCGG TTTGCGTATT GGGCGCCAGG GTGGTTTTTC TTTTCACCAG TGACACGGGC
     4681 AACAGCTGAT TGCCCTTCAC CGCCTGGCCC TGAGAGAGTT GCAGCAAGCG GTCCACGCTG
     4741 GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG ATGGTGGTTA ACGGCGGGAT ATAACATGAG
     4801 CTATCTTCGG TATCGTCGTA TCCCACTACC GAGATATCCG CACCAACGCG CAGCCCGGAC
     4861 TCGGTAATGG CGCGCATTGC GCCCAGCGCC ATCTGATCGT TGGCAACCAG CATCGCAGTG
     4921 GGAACGATGC CCTCATTCAG CATTTGCATG GTTTGTTGAA AACCGGACAT GGCACTCCAG
     4981 TCGCCTTCCC GTTCCGCTAT CGGCTGAATT TGATTGCGAG TGAGATATTT ATGCCAGCCA
     5041 GCCAGACGCA GACGCGCCGA GACAGAACTT AATGGGCCCG CTAACAGCGC GATTTGCTGG
     5101 TGACCCAATG CGACCAGATG CTCCACGCCC AGTCGCGTAC CATCTTCATG GGAGAAAATA
     5161 ATACTGTTGA TGGGTGTCTG GTCAGAGACA TCAAGAAATA ACGCCGGAAC ATTAGTGCAG
     5221 GCAGCTTCCA CAGCAATGGC ATCCTGGTCA TCCAGCGGAT AGTTAATGAT CAGCCCACTG
     5281 ACGCGTTGCG CGAGAAGATT GTGCACCGCC GCTTTACAGG CTTCGACGCC GCTTCGTTCT
     5341 ACCATCGACA CCACCACGCT GGCACCCAGT TGATCGGCGC GAGATTTAAT CGCCGCGACA
     5401 ATTTGCGACG GCGCGTGCAG GGCCAGACTG GAGGTGGCAA CGCCAATCAG CAACGACTGT
     5461 TTGCCCGCCA GTTGTTGTGC CACGCGGTTG GGAATGTAAT TCAGCTCCGC CATCGCCGCT
     5521 TCCACTTTTT CCCGCGTTTT CGCAGAAACG TGGCTGGCCT GGTTCACCAC GCGGGAAACG
     5581 GTCTGATAAG AGACACCGGC ATACTCTGCG ACATCGTATA ACGTTACTGG TTTCACATTC
     5641 ACCACCCTGA ATTGACTCTC TTCCGGGCGC TATCATGCCA TACCGCGAAA GGTTTTGCAC
     5701 CATTCGATGG TGTCCGGGAT CTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA
     5761 GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA
     5821 GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC
     5881 GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC
     5941 GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT
     6001 CGAGATCTCG ATCCCGCGAA ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC
     6061 AATTCCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGGCAGCAG
     6121 CCATCATCAT CATCATCACC TTAAACAGGT AGAAATTTTC ACCGATGGTT CGTGTCTGGG
     6181 CAATCCAGGA CCTGGGGGTT ACGGCGCTAT TTTACGCTAT CGCGGACGCG AGAAAACCTT
     6241 TAGCGCTGGC TACACCCGCA CCACCAACAA CCGTATGGAG TTGATGGCCG CTATTGTCGC
     6301 GCTGGAGGCG TTAAAAGAAC ATTGCGAAGT CATTTTGAGT ACCGACAGCC AGTATGTCCG
     6361 CCAAGGTATC ACCCAGTGGA TCCATAACTG GAAAAAACGT GGCTGGAAAA CCGCAGACAA
     6421 AAAACCAGTA AAAAATGTCG ATCTCTGGCA ACGTCTTGAT GCTGCATTGG GGCAGCATCA
     6481 AATCAAATGG GAATGGGTTA AAGGCCATGC CGGACACCCG GAAAACGAAC GCTGTGATGA
     6541 ACTGGCTCGT GCCGCGGCGA TGAATCCCAC ACTGGAAGAT ACAGGCTACC AAGTTGAAGT
     6601 TTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA
     6661 GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA
     6721 AGGAGGAACT ATATCCGGAC CCCAGGATAC ATAGATTACC ACAACTCCGA GCCCTTCCAC
     6781 CACTAGT
//