LOCUS       BBF10K_003570           8431 bp ds-DNA     circular     18-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     source          1..8431
                     /label="source"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      38..85
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    265..515
                     /label="incC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(518..1273)
                     /label="repE"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     exon            complement(518..1273)
                     /label="Translation 1273-518"
     rep_origin      1364..1583
                     /label="ori2"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    2003..2024
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        2039..2069
                     /label="lac promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    2077..2093
                     /label="lac operator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     2101..2117
                     /label="M13 rev"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2190..3035
                     /label="repL"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     exon            2190..3035
                     /label="Translation 2190-3035"
     misc_feature    3143..3294
                     /label="nnpp promoters"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             3334..4149
                     /label="KanR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     exon            3334..4149
                     /label="Translation 3334-4149"
     terminator      complement(4259..4302)
                     /label="bacterial terminator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     4320..4336
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      4337..4368
                     /label="tonB terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4389..8431
                     /label="TEV"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc
       61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt
      121 gtcgcgtatg catcgatcac gtgtccacgt tctttaatag tggactcttg ttccaaactg
      181 gaacaacact cggatcgatc cggcgcgcac cgtgggaaaa actccaggta gaggtacaca
      241 cgcggatagc caattcagag taataaactg tgataatcaa ccctcatcaa tgatgacgaa
      301 ctaacccccg atatcaggtc acatgacgaa gggaaagaga aggaaatcaa ctgtgacaaa
      361 ctgccctcaa atttggcttc cttaaaaatt acagttcaaa aagtatgaga aaatccatgc
      421 aggctgaagg aaacagcaaa actgtgacaa attaccctca gtaggtcaga acaaatgtga
      481 cgaaccaccc tcaaatctgt gacagataac cctcagacta tcctgtcgtc atggaagtga
      541 tatcgcggaa ggaaaatacg atatgagtcg tctggcggcc tttctttttc tcaatgtatg
      601 agaggcgcat tggagttctg ctgttgatct cattaacaca gacttgcagg aagcggcggc
      661 ggaagtcagg catacgctgg taactttgag gcagctggta acgctctatg atccagtcga
      721 ttttcagaga cacgatgcct gagccatccg gcttacgata ctgacacagg gattcgtata
      781 aacgcatggc atacggattg gtgatttctt ttgtttcact aagccgaaac tgcgtaaacc
      841 ggttctgtaa cccgataaag aagggaatga gatatgggtt gatatgtaca ctgtaaagcc
      901 ctctggatgg actgtgcgca cgtttgataa accaaggaaa agattcatag cctttttcat
      961 caccggcatc ctcttcaggg cgataaaaaa ccacttcctt ccccgcgaaa ctcttcaatg
     1021 cctgccgtat atccttactg gcttccgcag aggtcaatcc gaatatttca gcatatttag
     1081 caacatggat ctcgcagata ccgtcatgtt cctgtagggt gccatcagat tttctgatct
     1141 ggtcaacgaa cagatacagc atacgttttt gatcccggga gagactatat gccgcctcag
     1201 tgaggtcgtt tgactggacg attcgcgggc tatttttacg tttcttgtga ttgataaccg
     1261 ctgtttccgc catgacagat ccatgtgaag tgtgacaagt ttttagattg tcacactaaa
     1321 taaaaaagag tcaataagca gggataactt tgtgaaaaaa cagcttcttc tgagggcaat
     1381 ttgtcacagg gttaagggca atttgtcaca gacaggactg tcatttgagg gtgatttgtc
     1441 acactgaaag ggcaatttgt cacaacacct tctgtagaac cagcatggat aaaggcctac
     1501 aaggcgctct aaaaaagaag atctaaaaac tataaaaaaa ataattataa aaatatcccc
     1561 gtggataagt ggataacccc aagggaagtt ttttcaggca tcgtgtgtaa gcagaatata
     1621 taagtgctgt tccctggtgc ttcctcgctc actcgaaatt cccggggata gctttatgct
     1681 tgtaaaccgt tttgtgaaaa aatttttaaa ataaaaaagg ggacctctag ggtccccaat
     1741 taattagtaa tataatctat taaaggtcat tcaaaaggtc atccaccgga tcaattcccc
     1801 tgctcgcgca ggctgggtgc caagctctcg ggtaacatca aggcccgatc cttggagccc
     1861 ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca gatccttgac ccgcagttgc
     1921 aaaccctcac tgatccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1981 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     2041 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     2101 caggaaacag ctatgaccat gattacgcca agcgcggtac ctgtcgcggc aacgcgctaa
     2161 cagacgtagt aagaaccacc agcattgtaa tgctggctaa agtcactttc ctgagctgta
     2221 taacgatgag cgattttact ttttctggct atgaattggc ctgctttgta acacactccg
     2281 gtctatcccg tagcgccggg catatcctgt cgcaatgtgc aaatctcgcg gcaacaacca
     2341 gtgaatactt cattcacaag cctcaccgcc tgatcgcggc agaaactggt tatagccaat
     2401 caaccgtcgt tcgtgcattc cgtgaagctg taaacaaagg tatcctgtct gtagagattg
     2461 ttatcggcga tcaccgtgaa cgtcgcgcta acctgtaccg gtttacacca tcctttttgg
     2521 ccttcgcaca acaagccaaa aatgcgctga tagaaagcaa attaaagatc tcttcagcgg
     2581 caaccaaggt taaagctgtt ctcgctaaga cattggcttt atttaatttt ttatccacac
     2641 ccccatgtca aaatgatacc ccctccccct gtcaggatga cgtggcaata aagaataaga
     2701 agtcacaagt taaaaaaaca aaaagatcag tttccggcgg tgccggaaca accagcctca
     2761 aaaaattgac ttcatggatc gctaaggcaa aagcaaaggc tgacaatctg cggttatcca
     2821 aaaaacgcac tcaaaaacat gagttcaagc agaaagtaga ggcggctgcg cggaaatatg
     2881 cttacctgaa gaacaagcgt tcgcctgata ttggcgggat atcaaacttc gataacctac
     2941 cgcattgcat gacggtaaac gaagctctta atgcggtttt agccaaaaat aaagataacg
     3001 aacaatgggg tataccggca ggattcagag ggtaatgaat tgctctaatt ataaccatgc
     3061 atactttcaa cacctctagt ttgccatgag gcaaactcat aggtgtcctg gtaagaggac
     3121 actgttgcca aaactggacg ccccattatt gcaattaata aacaactaac ggacaattct
     3181 acctaacaat aagtggagtt gcggccggcc gcgatccgct agcaaagcca cgttgtgtct
     3241 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     3301 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
     3361 tgctcgcgcc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     3421 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     3481 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     3541 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     3601 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta
     3661 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     3721 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgcctc
     3781 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3841 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactgtt gccattctca
     3901 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3961 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     4021 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     4081 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     4141 tttttctaat cagaattggt taattggttg taacactggc gagctctgcc acctgacgtc
     4201 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     4261 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctgcacctg caccagtcag
     4321 taaaacgacg gccagtagtc aaaagcctcc gaccggaggc ttttgacttg gttcaggtgg
     4381 agtgggagTT AATTAACATT ACTCGCATCC ATTCTCAGGC TGTCTCGTCT CGTCTCGACA
     4441 CCATCGAATG GTGCAAAACC TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT
     4501 TCAGGGTGGT GAATGTGAAA CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT
     4561 CTTATCAGAC CGTTTCCCGC GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG
     4621 AAAAAGTGGA AGCGGCGATG GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC
     4681 TGGCGGGCAA ACAGTCGTTG CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC
     4741 CGTCGCAAAT TGTCGCGGCG ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG
     4801 TGTCGATGGT AGAACGAAGC GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG
     4861 CGCAACGCGT CAGTGGGCTG ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG
     4921 TGGAAGCTGC CTGCACTAAT GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA
     4981 TCAACAGTAT TATTTTCTCC CATGAAGATG GTACGCGACT GGGCGTGGAG CATCTGGTCG
     5041 CATTGGGTCA CCAGCAAATC GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC
     5101 TGCGTCTGGC TGGCTGGCAT AAATATCTCA CTCGCAATCA AATTCAGCCG ATAGCGGAAC
     5161 GGGAAGGCGA CTGGAGTGCC ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG
     5221 GCATCGTTCC CACTGCGATG CTGGTTGCCA ACGATCAGAT GGCGCTGGGC GCAATGCGCG
     5281 CCATTACCGA GTCCGGGCTG CGCGTTGGTG CGGATATCTC GGTAGTGGGA TACGACGATA
     5341 CCGAAGATAG CTCATGTTAT ATCCCGCCGT TAACCACCAT CAAACAGGAT TTTCGCCTGC
     5401 TGGGGCAAAC CAGCGTGGAC CGCTTGCTGC AACTCTCTCA GGGCCAGGCG GTGAAGGGCA
     5461 ATCAGCTGTT GCCCGTGTCA CTGGTGAAAA GAAAAACCAC CCTGGCGCCC AATACGCAAA
     5521 CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC
     5581 TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTAAGTT AGCTCACTCA TTAGGCACAA
     5641 TTCTCATGTT TGACAGCTTA TCATCGACTG CACGGTGCAC CAATGCTTCT GGCGTCAGGC
     5701 AGCCATCGGA AGCTGTGGTA TGGCTGTGCA GGTCGTAAAT CACTGCATAA TTCGTGTCGC
     5761 TCAAGGCGCA CTCCCGTTCT GGATAATGTT TTTTGCGCCG ACATCATAAC GGTTCTGGCA
     5821 AATATTCTGA AATGAGCTGT TGACAATTAA TCATCGGCTC GTATAATGTG TGGAATTGTG
     5881 AGCGGATAAC AATTTCACAC AGGAAACAGC CAGTCCGTTT AGGTGTTTTC ACGAGCACTT
     5941 CACCAACAAG GACCATAGAT TATGAAAACT GAAGAAGGTA AACTGGTAAT CTGGATTAAC
     6001 GGCGATAAAG GCTATAACGG CCTCGCTGAA GTCGGTAAGA AATTCGAGAA AGATACCGGA
     6061 ATTAAAGTCA CCGTTGAGCA TCCGGATAAA CTGGAAGAGA AATTCCCACA GGTTGCGGCA
     6121 ACTGGCGATG GCCCTGACAT TATCTTCTGG GCACACGACC GCTTTGGTGG CTACGCTCAA
     6181 TCTGGCCTGT TGGCTGAAAT CACCCCGGAC AAAGCGTTCC AGGACAAGCT GTATCCGTTT
     6241 ACCTGGGATG CCGTACGTTA CAACGGCAAG CTGATTGCTT ACCCGATCGC TGTTGAAGCG
     6301 TTATCGCTGA TTTATAACAA AGATCTGCTG CCGAACCCGC CAAAAACCTG GGAAGAGATC
     6361 CCGGCGCTGG ATAAAGAACT GAAAGCGAAA GGTAAGAGCG CGCTGATGTT CAACCTGCAA
     6421 GAACCGTACT TCACCTGGCC GCTGATTGCT GCTGACGGGG GTTATGCGTT CAAGTATGAA
     6481 AACGGCAAGT ACGACATTAA AGACGTGGGC GTGGATAACG CTGGCGCGAA AGCGGGTCTG
     6541 ACCTTCCTGG TTGACCTGAT TAAAAACAAA CACATGAATG CAGACACCGA TTACTCCATC
     6601 GCAGAAGCTG CCTTTAATAA AGGCGAAACA GCGATGACCA TCAACGGCCC GTGGGCATGG
     6661 TCCAACATCG ACACCAGCAA AGTGAATTAT GGTGTAACGG TACTGCCGAC CTTCAAGGGT
     6721 CAACCATCCA AACCGTTCGT TGGCGTGCTG AGCGCAGGTA TTAACGCCGC CAGTCCGAAC
     6781 AAAGAGCTGG CAAAAGAGTT CCTCGAAAAC TATCTGCTGA CTGATGAAGG TCTGGAAGCG
     6841 GTTAATAAAG ACAAACCGCT GGGTGCCGTA GCGCTGAAGT CTTACGAGGA AGAGTTGGCG
     6901 AAAGATCCAC GTATTGCCGC CACCATGGAA AACGCCCAGA AAGGTGAAAT CATGCCGAAC
     6961 ATCCCGCAGA TGTCCGCTTT CTGGTATGCC GTGCGTACTG CGGTGATCAA CGCCGCCAGC
     7021 GGTCGTCAGA CTGTCGATGA AGCCCTGAAA GACGCGCAGA CTAATTCGAG CTCGAACAAC
     7081 AACAACAATA ACAATAACAA CAACCTCGGG ATCGAGGGAA GGGGAGAAAA TCTTTATTTT
     7141 CAAGGTCATC ATCATCATCA TCATCATGGA GAAAGCTTGT TTAAGGGGCC GCGTGATTAC
     7201 AACCCGATAT CGAGCACCAT TTGTCATTTG ACGAATGAAT CTGATGGGCA CACAACATCG
     7261 TTGTATGGTA TTGGATTTGG TCCCTTCATC ATTACAAACA AGCACTTGTT TAGAAGAAAT
     7321 AATGGAACAC TGTTGGTCCA ATCACTACAT GGTGTATTCA AGGTCAAGAA CACCACGACT
     7381 TTGCAACAAC ACCTCATTGA TGGGAGGGAC ATGATAATTA TTCGCATGCC TAAGGATTTC
     7441 CCACCATTTC CTCAAAAGCT GAAATTTAGA GAGCCACAAA GGGAGGAACG CATATGTCTT
     7501 GTGACAACCA ACTTCCAAAC TAAGAGCATG TCTAGCATGG TGTCAGACAC CAGTTGCACA
     7561 TTCCCTTCAT CTGATGGCAT ATTCTGGAAG CATTGGATTC AAACCAAGGA TGGGCAGTGT
     7621 GGCAGTCCAT TAGTATCAAC TAGAGATGGG TTCATTGTTG GTATACACTC AGCATCGAAT
     7681 TTCACCAACA CAAACAATTA TTTCACAAGC GTGCCGAAAA ACTTCATGGA ATTGTTGACA
     7741 AATCAGGAGG CGCAGCAGTG GGTTAGTGGT TGGCGATTAA ATGCTGACTC AGTATTGTGG
     7801 GGGGGCCATA AAGTTTTCAT GGTGAAACCT GAGGAACCTT TTCAGCCAGT TAAGGAAGCG
     7861 ACTCAACTCA TGAATCGTCG TCGCCGTCGC TAATAAGGAT CCTCTAGAGT CGACCTGCAG
     7921 GCAAGCTTGG CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC
     7981 CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC
     8041 CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCA GCTTGGCTGT
     8101 TTTGGCGGAT GAGATAAGAT TTTCAGCCTG ATACAGATTA AATCAGAACG CAGAAGCGGT
     8161 CTGATAAAAC AGAATTTGCC TGGCGGCAGT AGCGCGGTGG TCCCACCTGA CCCCATGCCG
     8221 AACTCAGAAG TGAAACGCCG TAGCGCCGAT GGTAGTGTGG GGACTCCCCA TGCGAGAGTA
     8281 GGGAACTGCC AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT
     8341 TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCCCAGG ATACATAGAT
     8401 TACCACAACT CCGAGCCCTT CCACCACTAG T
//