MMSYN1_0238

30S ribosomal protein S4
BBF10K_000101
source
Synthetic bacterium JCVI-Syn3.0

With S5 and S12 plays an important role in translational accuracy.

attr.
Anton Jackson-Smith and Bioe80 class

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-MMSYN1_0238
plasmid size
2650
insert size
627
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
UniProt
Available Elsewhere
FALSE

intellectual property

No Value